Skip to main content
SpringerLink
Log in

First report of Flavescence dorée phytoplasma identification and characterization in three species of leafhoppers

  • Short Communication
  • Published:
Journal of Plant Pathology Aims and scope Submit manuscript

Abstract

Phytoplasma strains were sporadically found in specimens of Hishimonus hamatus, Japananus hyalinus and Graphocephala fennahi caught during an entomological survey conducted in Southern Switzerland on the edges and woods surrounding cultivated vineyards in 2017. The subsequent molecular characterization of the pathogens allowed to identify them as strains strictly associated to Flavescence dorée, a serious European grapevine disease, transmitted from vine to vine by the leafhopper Scaphoideus titanus. In particular, M12 and M50 genotypes were found in H. hamatus and J. hyalinus, respectively, while G. fennahi harboured mixed phytoplasma genotypes with at least two different Flavescence dorée isolates. The potential importance of these findings in the frame of the Flavescence dorée epidemics in Europe is discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Availability of data and material

The sequences obtained in this study were deposited into the NCBI under the accession numbers MW367151-MW367152.

References

  • Angelini E, Clair D, Borgo M, Bertaccini A, Boudon-Padieu E (2001) Flavescence dorée in France and Italy - Occurrence of closely related phytoplasma isolates and their near relationships to Palatinate grapevine yellows and an alder phytoplasma. Vitis 40:79–86

    CAS  Google Scholar 

  • Angelini E, Bianchi GL, Filippin L, Morassutti C, Borgo M (2007) A new TaqMan method for the identification of phytoplasmas associated with grapevine yellows by real-time PCR assay. J Microbiol Methods 68:613–622

    Article  CAS  Google Scholar 

  • Angelini E, Constable F, Duduk B, Fiore N, Quaglino F, Bertaccini A (2018) Grapevine phytoplasmas. In: Rao GP, Bertaccini A, Fiore N, Liefting LW (eds) Phytoplasmas: Plant pathogenic bacteria – I: Characterisation and Epidemiology of Phytoplasma Associated Diseases. Springer, Singapore, pp 123–151

    Chapter  Google Scholar 

  • Arnaud G, Malembic-Maher S, Salar P, Bonnet P, Maixner M, Marcone C, Boudons-Padieu E, Fossaic X (2007) Multilocus sequence typing confirms the close genetic interrelatedness of three distinct flavescence doree phytoplasma strain clusters and group 16SrV phytoplasmas infecting grapevine and alder in Europe. Appl Environ Microbiol 73:4001–4010

    Article  CAS  Google Scholar 

  • Bonfield JK, Smith KF, Staden R (1995) A new DNA sequence assembly program. Nucleic Acids Res 23:4992–4999

    Article  CAS  Google Scholar 

  • Casati P, Jermini M, Quaglino F, Corbani G, Schaerer S, Passera A, Bianco PA, Rigamonti IE (2017) New insights on Flavescence dorée phytoplasma ecology in the vineyard agro-ecosystem in southern Switzerland. Ann Appl Biol 171:37–51

    Article  CAS  Google Scholar 

  • Chuche J, Sauvion N, Thiéry D (2017) Mixed xylem and phloem sap ingestion in sheath-feeders as normal dietary behavior: Evidence from the leafhopper Scaphoideus titanus. J Insect Physiol 102:62–72

    Article  CAS  Google Scholar 

  • Endrestøl A (2017) Graphocephala fennahi Young, 1977 (Hemiptera, Cicadellidae) and Seifertia azaleae (Peck) Partr. Morgan-Jones (Ascomycota, Dothideomycetes) in Norway. Norw J Entomol 64:112–129

    Google Scholar 

  • Filippin L, Jović J, Cvrković T, Forte V, Clair D, Toševski I, Boudon-Padieu E, Borgo M, Angelini E (2009) Molecular characteristics of phytoplasmas associated with ‘Flavescence dorée’ in clematis and grapevine and preliminary results on the role of Dictyophara europaea as a vector. Plant Pathol 58:826–837

    Article  CAS  Google Scholar 

  • Galetto L, Pegoraro M, Marzachì C, Rossi E, Lucchi A, Bosco D (2019) Potential role of the alien planthopper Ricania speculum as vector of Flavescence dorée phytoplasma. Eur J Plant Pathol 154:1103–1110

    Article  Google Scholar 

  • Gatineau F, Larrue J, Clair D, Lorton F, Richard-Molard M, Boudon-Padieu E (2001) A new natural planthopper vector of stolbur phytoplasma in the genus Pentastiridius (Hemiptera: Cixiidae). Eur J Plant Pathol 107:63–271

    Article  Google Scholar 

  • Hamilton KGA (1985) The Graphocephala coccinea complex in North America (Homoptera, Auchenorrhyncha, Cicadellidae). Entomol Abh Staatliches Museum Tierkunde Dresden 49(6):105–111

    Google Scholar 

  • Jakovljević M, Jović J, Krstić O, Mitrović M, Marinković S, Toševski I, Cvrković T (2020) Diversity of phytoplasmas identified in the polyphagous leafhopper Euscelis incisus (Cicadellidae, Deltocephalinae) in Serbia: Pathogen inventory, epidemiological significance and vectoring potential. Eur J Plant Pathol 156:201–221

    Article  Google Scholar 

  • Jermini M, Schaerer S, Casati P, Corbani G, Quaglino F, Rigamonti I, Bianco PA (2017) Orientus ishidae, un nouveau vecteur de la flavescence dorée au Tessin. Rev Suisse Vitic Arboric Hortic 49:280–288

    Google Scholar 

  • Jermini M, Conedera M, Rizzoli A, Belgeri E, Filippin L, Angelini E (2019) Potential role of Orientus ishidae in the “flavescence dorée” epidemics in Ticino, Switzerland. Phytopathogenic Mollicutes 9:67–68

    Article  Google Scholar 

  • Krstić O, Cvrković T, Mitrović M, Radonjić S, Hrnčić S, Toševski I, Jović J (2018) Wolbachia infection in natural populations of Dictyophara europaea, an alternative vector of grapevine Flavescence doree phytoplasma: effects and interactions. Ann Appl Biol 172:47–64

    Article  Google Scholar 

  • Lessio F, Picciau L, Gonella E, Mandrioli M, Tota F, Alma A (2016) The mosaic leafhopper Orientus ishidae: host plants, spatial distribution, infectivity and transmission of 16SrV phytoplasmas to vines. Bull Insectol 69:277–289

    Google Scholar 

  • Malembic-Maher S, Desque D, Khalil D, Salar P, Bergey B, Danet JL, Duret S, Dubrana-Ourabah MP, Beven L, Ember I, Acs Z, Della Bartola M, Materazzi A, Filippin L, Krnjajic S, Krstić O, Toševski I, Lang F, Jaraush B, Kölber M, Jović J, Angelini E, Arricau-Bouvery N, Maixner M, Foissac X (2020) When a Palearctic bacterium meets a Nearctic insect vector: Genetic and ecological insights into the emergence of the grapevine Flavescence dorée epidemics in Europe. PLoS Pathog 16(3):e1007967

    Article  CAS  Google Scholar 

  • Nickel H (2003) The Leafhoppers and Planthoppers of Germany (Hemiptera: Auchenorrhyncha): Patterns and strategies in a highly diverse group of phytophagous insects. Ser Faun 28:460

    Google Scholar 

  • Plavec J, Budinšćak Ž, Križanac I, Škorić D, Foissac X, Šeruga Musić M (2019) Multilocus sequence typing reveals the presence of three distinct flavescence dorée phytoplasma genetic clusters in Croatian vineyards. Plant Pathol 68:18–30

    Article  CAS  Google Scholar 

  • Reisenzein H, Strauss G (2019) Sporadic outbreaks of “flavescence dorée” in Austrian vineyards and the role of Phlogottetix cyclops as a potential vector. Phytopathogenic Mollicutes 9:61–62

    Article  Google Scholar 

  • Rizza S, Rossi M, Spallino RE, Tedeschi R, Marzachì C, Catara V, Tessitori M (2021) Ecology-based analysis of a recent association between Spartium junceum and 16SrV phytoplasma. Plant Pathol 70:305–317

    Article  CAS  Google Scholar 

  • Rossi M, Pegoraro M, Ripamonti M, Abbà S, Beal D, Giraudo A, Veratti F, Malembic-Maher S, Salar P, Bosco D, Marzachì C (2019) Genetic diversity of Flavescence dorée phytoplasmas at the vineyard scale. Appl Environ Microbiol 85:e03123-e3218

    Article  CAS  Google Scholar 

  • Schvester D, Carle P, Moutous G (1961) Sur la transmission de la Flavescence doree de la vigne par une Cicadelle. Compte Rendu Acad Agricol Fr 67:1021–1024

    Google Scholar 

  • Seljak G (2013) Hishimonus hamatus Kuoh (Hemiptera: Cicadellidae): A new alien leafhopper in Europe. Acta Entomol Slov 21:123–130

    Google Scholar 

  • Špryňar P (2005) First records of the rhododendron leafhopper (Graphocephala fennahi) (Hemiptera: Auchenorrhyncha: Cicadellidae) from the Czech Republic. Plant Prot Sci 41:38–41

    Article  Google Scholar 

  • Strauss G, Reisenzein H (2018) First detection of Flavescence dorée phytoplasma in Phlogotettix cyclops (Hemiptera, Cicadellidae) and considerations on its possible role as vector in Austrian vineyards. IOBC-WPRS Bull 139:12–21

    Google Scholar 

  • Trivellone V, Knop E, Turrini T, Andrey A, Humbert JY, Kunz G (2015) New and remarkable leafhoppers and planthoppers (Hemiptera: Auchenorrhyncha) from Switzerland. Mitt Schweiz Entomol Ges 88:273–284

    Google Scholar 

  • Trivellone V, Filippin L, Narduzzi-Wicht B, Angelini E (2016) A regional-scale survey to define the known and potential vectors of grapevine yellow phytoplasmas in vineyards South of Swiss Alps. Eur J Plant Pathol 145:915–927

    Article  CAS  Google Scholar 

  • Weintraub PG, Beanland L (2006) Insect vectors of phytoplasmas. Annu Rev Entomol 51:91–111

    Article  CAS  Google Scholar 

  • Xing JC, Dai RH, Li ZZ (2010) Two new species of the genus Japananus Ball, 1931 (Hemiptera: Cicadellidae: Deltocephalinae). Zootaxa 2415:63–68

    Article  Google Scholar 

  • Young DA (1977) Taxonomic study on the Cicadellinae (Homoptera: Cicadellidae), part 2. New World Cicadellini and the genus Cicadella. Tech Bull NC Agric Exp Stn 239:1135

    Google Scholar 

Download references

Funding

This work was performed in the frame of the Flavid initiative of the Euphresco network and was partially funded by the Swiss Federal Office for Agriculture FOAG (grant 627001075).

Author information

Authors and Affiliations

  1. Council for Agriculture Research and Economics, Research Centre for Viticulture and Enology, CREA, Conegliano, TV, Italy

    Elena Belgeri, Elisa Angelini & Luisa Filippin

  2. Agroscope – Campus di ricerca, Cadenazzo, Switzerland

    Elena Belgeri, Attilio Rizzoli & Mauro Jermini

  3. Snow and Landscape Research WSL – Campus di ricerca, Swiss Federal Institute for Forest, Cadenazzo, Switzerland

    Attilio Rizzoli

  4. Department of Food, Environmental and Nutritional Sciences, Università Degli Studi Di Milano, Milano, Italy

    Ivo Ercole Rigamonti

Authors
  1. Elena Belgeri
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Attilio Rizzoli
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mauro Jermini
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Elisa Angelini
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Luisa Filippin
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Ivo Ercole Rigamonti
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Luisa Filippin.

Ethics declarations

Conflicts of interest

The authors declare that they have no conflict of interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Belgeri, E., Rizzoli, A., Jermini, M. et al. First report of Flavescence dorée phytoplasma identification and characterization in three species of leafhoppers. J Plant Pathol 104, 375–379 (2022). https://doi.org/10.1007/s42161-021-01012-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s42161-021-01012-y

Keywords

Search

Navigation