Abstract
As an endemic fruit tree in China, the Chinese dwarf cherry (Cerasus humilis (Bge.) Sok.) has substantial ecological and economic value. In 2018, characteristic symptoms of the crown gall of the Chinese dwarf cherry were observed in a nursery base located in Ningxia Hui Autonomous Region, China. The occurrence of the disease among seedlings was observed to be 9.48%. Diseased plants were collected and brought to the laboratory for the isolation of the pathogens. In this study, the isolates were identified by a combination of morphological, biochemical, pathogenicity-related, as well as molecular methods. The isolated strains were white, circular, domed, and glistening. Using 16S rRNA gene sequence analysis and biochemical tests, five strains were identified as Agrobacterium tumefaciens/ biovar 1. Polymerase chain reaction (PCR) was used to detect pathogenicity-associated genes in isolates through the amplification of the partial nucleotide sequences of tms2, virD2 and ipt genes, which proved that the isolates contained the Ti plasmid. In the pathogenicity assay, the crown gall symptom developed on the young stem of tomato and sunflower after inoculation. Based on its morphological, biochemical, pathogenicity and genetic characteristics, the pathogen of the Chinese dwarf cherry crown gall was identified to be A. tumefaciens/ biovar 1.
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Acknowledgements
Education science and technology innovation project of Gansu Province (grant No. GSSYLXM-02) and Key research and development projects in Ningxia Autonomous Region (grant No.2020BBF02027).
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Author Jie Bai declares that she has no conflict of interest. Author Tao Yao declares that he has no conflict of interest. Author Xiaojun Lan declares that he has no conflict of interest. Author Yanshan Yang declares that she has no conflict of interest. Author Zhanjun Wang declares that he has no conflict of interest. Author Xinyou Wang declares that he has no conflict of interest.
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Bai, J., Yao, T., Lan, X. et al. Isolation of Agrobacterium tumefaciens/ biovar 1 from the crown gall of Cerasus humilis in China. J Plant Pathol 104, 141–148 (2022). https://doi.org/10.1007/s42161-021-00947-6
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DOI: https://doi.org/10.1007/s42161-021-00947-6