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Morpho-molecular variability and host reactivity of Albugo candida isolates infecting Brassica juncea genotypes in India

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Abstract

To generate information on variability in the pathogen causing white rust disease in Brassica species and their ability to infect Brassica juncea genotypes, thirteen isolates of Albugo candida infecting B. juncea and one isolate of Wilsonia bliti infecting Amaranthus blitum were collected from different rapeseed-mustard growing regions of India. These isolates were characterized based on different morphological data (sporangial shape and size; shape, size, and color of the pustule) and molecular data (cytochrome c oxidase subunit II (COX2) of mtDNA and internal transcribed spacer (ITS) region of rDNA). All the sporangia were globose or spherical to ellipsoidal with a size ranging from 199.01 μm2 to 357.23 μm2. White to creamy colored pustules were observed with a diameter ranging from 0.5–3.0 mm. Sequence analysis revealed that the Indian isolates of A. candida collected from B. juncea showed close identity with the Korean isolates infecting B. juncea. W. blilti isolate collected from A. blitum form a distinct clade with A. amaranthi infecting Amaranthus spinosus from Korea. Further, the pathogenic reaction of ten A. candida isolates was recorded on thirty genotypes of B. juncea that exhibited differential responses to the tested isolates. The three genotypes, PDZ-3, Pusa Karishma, and Donskaja were found to be resistant against all the ten isolates, which can further be used as a resistance source. PDZ-2 genotype was found to be susceptible only against the Ac-Hr isolate while the genotype BioYSR shows a susceptible reaction against Ac-Skn and Ac-Wltn isolates only. This set of thirty genotypes was able to differentiate the ten isolates of A. candida into four physiological races (AcRBj1 to AcRBj4) based on the disease reaction. The information generated through this study will be helpful for further study on identification and molecular mapping of resistance gene(s), their deployment over time and space, and, thus, better management of white rust disease in B. juncea.

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Acknowledgments

The authors are thankful to the Director, ICAR-IARI, Pusa, New Delhi-12, India, for providing financial assistance to carry out this research work.

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Authors and Affiliations

  1. Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India

    Oinam Washington Singh, Deeba Kamil, Vaibhav Kumar Singh, Thokala Prameela Devi & Lakshman Prasad

  2. Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India

    Naveen Singh

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  1. Oinam Washington Singh
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  2. Naveen Singh
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  3. Deeba Kamil
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  4. Vaibhav Kumar Singh
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Supplementary Information

Supplementary Fig. 1

Different pustule development patterns of various A. candida isolates on cotyledonary lobes of B. juncea host (PNG 1522 kb)

High resolution image (TIF 496 kb)

Supplementary Fig. 2

Identity matrix of COX2 sequences from 13 Indian A. candida isolates and 25 NCBI sequences used for comparison (JPG 558 kb)

Supplementary Fig. 3

Identity matrix of ITS sequences from 10 Indian A. candida isolates and 23 NCBI sequences used for comparison (JPG 712 kb)

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Singh, O.W., Singh, N., Kamil, D. et al. Morpho-molecular variability and host reactivity of Albugo candida isolates infecting Brassica juncea genotypes in India. J Plant Pathol 103, 139–153 (2021). https://doi.org/10.1007/s42161-020-00690-4

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