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Analysis of the complete genome sequence of cucumber mosaic virus from Vinca minor and Wisteria sinensis in Iran

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Abstract

We present here the first complete sequence of cucumber mosaic virus (CMV) from RNA sequencing of a dwarf periwinkle (Vinca minor) and a Wisteria sinensis in Iran by de novo assembly and annotation of contigs. The tripartite genome of CMV-Ir-VM and Ir-WS consists of a 3391-nucleotide (nt) RNA1, a 3038-nt RNA2, and a 2197-nt RNA3 segment. The sequence comparisons and phylogenetic analyses revealed that all three RNA segments of these two isolates belonged to the CMV subgroup II. RNA1, RNA2, and RNA3 of these two isolates, respectively shared 97.61–99.06%, 97.83–98.88%, and 97.19–98.86% nucleotide identities to those of CMV subgroup II isolates. In genetic analysis, the 2b gene revealed more genetic variability (π  = 0.2614) compared with other regions. Our results indicated that purifying selection pressure was the major force acting upon the CMV genome, although some positively selected sites were identified for all encoded proteins.

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Author notes

  1. Sharifeh Dabiri and Zohreh Moradi contributed equally to this work as first authors

Authors and Affiliations

  1. Department of Plant Pathology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, 91775-1163, Iran

    Sharifeh Dabiri, Mohsen Mehrvar & Mohammad Zakiaghl

  2. Department of Plant Pathology, Faculty of Crop Sciences, Sari Agricultural Sciences and Natural Resources University, P. O. BOX: 578, Sari, Iran

    Zohreh Moradi

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  1. Sharifeh Dabiri
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  2. Zohreh Moradi
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Correspondence to Mohsen Mehrvar.

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Dabiri, S., Moradi, Z., Mehrvar, M. et al. Analysis of the complete genome sequence of cucumber mosaic virus from Vinca minor and Wisteria sinensis in Iran. J Plant Pathol 102, 1263–1268 (2020). https://doi.org/10.1007/s42161-020-00650-y

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