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Diagrammatic scale for assessment of alternaria brown spot severity on tangerine leaves

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Abstract

Alternaria brown spot (ABS) is one of the main diseases of tangerine worldwide. It is necessary to use a practical and reliable method to quantify the disease severity of ABS and thus suggest management strategies. Thus, a diagrammatic scale (DS) constructed with color photographs was developed and validated to quantify the disease severity. Leaves were collected from crops and a nursery with different levels of ABS symptoms to determine the real severity digitally. The scale levels were determined according to the minimum and maximum severity values found and based on the frequency distribution of severity values. The DS validation was carried out by eleven assessors who estimated the severity of 50 leaves with different symptom levels. One assessment was performed without the DS and two using the DS at seven day intervals. The precision, accuracy, repeatability and reproducibility of the estimates were measured. The developed DS has nine score levels: 0 (0%), 1 (0.1–0.49%), 2 (0.5–1.0%), 3 (1.1–2.0%), 4 (2.1–4.0%), 5 (4.1–8%), 6 (8.1–18%), 7 (18.1–30%) and 8 (30.1–50%). The linear regression and Lin’s concordance correlation coefficient (LCCC) demonstrated an improved accuracy, precision repeatability and reproducibility of the estimates using the DS. The scale is appropriate, useful and reliable to visually estimate with the ABS severity in tangerine leaves.

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References

  • Ajiro N, Miyamoto Y, Masunaka A, Tsuge T, Yamamoto M, Ohtani K, Fukumoto T, Gomi K, Peever TL, Izumi Y, Tada Y, Akimitsu K (2010) Role of the host-selective ACT-toxin synthesis gene ACTTS2 encoding an enoyl-reductase in pathogenicity of the tangerine pathotype of Alternaria alternata. Phytopathology 100(2):120–126

    CAS  PubMed  Google Scholar 

  • Akimitsu K, Peever TL, Timmer LW (2003) Molecular, ecological and evolutionary approaches to understanding Alternaria diseases of citrus. Mol Plant Pathol 4:435–446

    CAS  PubMed  Google Scholar 

  • Belan LL, Pozza EA, Freitas MLDO, Souza RM, Jesus Junior WC, Oliveira JM (2014) Diagrammatic scale for assessment of bacterial blight in coffee leaves. J Phytopathol 162:801–810

    Google Scholar 

  • Berger RD (1980) Measuring disease intensity. In: Teng PS, Krupa SV (eds) Crop loss assessment. University of Minnesota, St. Paul, pp 28–31

    Google Scholar 

  • Bock CH, Cook AZ, Parker PE, Gottwald TR (2009) Automated image analysis of the severity of foliar citrus canker symptoms. Plant Dis 93:660–665

    CAS  PubMed  Google Scholar 

  • Bock CH, Poole GH, Parker PE, Gottwald TR (2010) Plant disease severity estimated visually, by digital photography and image analysis, and by hyperspectral imaging. Crit Rev Plant Sci 29:59–107

    Google Scholar 

  • Braido R, Gonçalves-Zuliani AM, Janeiro V, Carvalho SA, Junior JB, Bock CH, Nunes WMC (2014) Development and validation of standard area diagrams as assessment aids for estimating the severity of citrus canker on unripe oranges. Plant Dis 98:1543–1550

    PubMed  Google Scholar 

  • Campbell CL, Madden LV (1990) Introduction to plant disease epidemiology. Wiley, New York

    Google Scholar 

  • Campos VA, Perina FJ, Alves E, Sartorelli J, Moura AM, Oliveira DF (2014) Anadenanthera colubrina (Vell.) Brenan produces steroidal substances that are active against Alternaria alternata (Fr.) Keissler and that may bind to oxysterol-binding proteins. Pest Manag Sci 70:1815–1822

    CAS  PubMed  Google Scholar 

  • Capucho AS, Zambolim L, Duarte HSS, Vaz GRO (2011) Development and validation of a standard area diagram set to estimate severity of leaf rust in Coffea arabica and C. canephora. Plant Pathol 60:1144–1150

    Google Scholar 

  • Carvalho DD, Alves E, Batista TR, Camargos RB, Lopes EA (2008) Comparison of methodologies for conidia production by Alternaria alternata from citrus. Braz J Microbiol 39:792–798

    PubMed  PubMed Central  Google Scholar 

  • Carvalho DDC, Alves E, Camargos RB, Oliveira DF, Scolforo JRS, de Carvalho DA, Batista TRS (2011) Plant extracts to control Alternaria alternata in Murcott tangor fruits. Rev Iberoam Micol 28:173–178

    PubMed  Google Scholar 

  • Correia KC, Queiroz JVJ, Martins RB, Nicoli A, Del Ponte EM, Michereff SJ (2017) Development and evaluation of a standard area diagram set for the severity of phomopsis leaf blight on eggplant. Eur J Plant Pathol 149(2):269–276

    Google Scholar 

  • Del Ponte EM, Pethybridge SJ, Bock CH, Michereff SJ, Machado FJ, Spolti P (2017) Standard area diagrams for aiding severity estimation: Scientometrics, Pathosystems and methodological trends in the last 25 years. Phytopathology 107:10 1161-1174

    Google Scholar 

  • Domiciano GP, Duarte HSS, Moreira EN, Rodrigues FA (2014) Development and validation of a set of standard area diagrams to aid in estimation of spot blotch severity on wheat leaves. Plant Pathol 63:922–928

    Google Scholar 

  • Duarte HSS, Zambolim L, Capucho AS, Júnior AFN, Rosado AWC, Cardoso CR, Paul PA, Mizubuti SG (2013) Development and validation of a set of standard area diagrams to estimate severity of potato early blight. Eur J Plant Pathol 137:249–257

    Google Scholar 

  • Fantin LH, Braga K, Canteri MG, Dias AR, Borges EP (2018) Development and validation of diagrammatic scale to assess target spot severity in cotton. Australas Plant Path https://doi.org/10.1007/s13313-018-0576-6

    CAS  Google Scholar 

  • Gomes AMA, Michereff SF, Mariano RLR (2004) Elaboração e validação de escala diagramática para cercosporiose da alface. Summa Phytopathol 30(1):38–42

  • Horsfall JC, Barratt RW (1945) An improved grading system for measuring plant diseases. Phytopathology 35:655–665

    Google Scholar 

  • Kohmoto K, Itoh Y, Shimomura N, Kondoh Y, Otani H, Kodama M, Nishimura S, Nakatsuka S (1993) Isolation and biological activities of two host-specific toxins from the tangerine pathotype of Alternaria alternata. Phytopathology 83:495–502

    CAS  Google Scholar 

  • Kranz J (1988) Measuring plant disease. In: Kranz J, Rotem J (eds) Experimental techniques in plant disease epidemiology. Springer Verlag, Heidelberg, pp 35–50

    Google Scholar 

  • Lage DAC, Marouelli WA, Duarte HSS, Café-Filho AC (2015) Standard area diagrams for assessment of powdery mildew severity on tomato leaves and leaflets. Crop Prot 67:26–34

    Google Scholar 

  • Lin LI (1989) A concordance correlation coefficient to evaluate reproducibility. Biometrics 45(1):255–268

    CAS  PubMed  Google Scholar 

  • Madden LV, Hughes G, van den Bosch F (2007) The study of plant disease epidemics. American Phytopathology Society Press, St Paul

    Google Scholar 

  • Martelli IB, Pacheco CA, Bastianel M, Schinor EH, Conceição PM, Azevedo FA (2016) Diagrammatic scale for assessing foliar symptoms of alternaria brown spot in citrus. Agron Sci Biotech 2:57–61

    Google Scholar 

  • Martins MC, Guerzoni RA, Camara GDS, Mattiazzi P, Lourenço AS, Amorim L (2004) Diagrammatic scale for assessment of late season leaf diseases in soybean. Fitopatol Bras 29:179–184

    Google Scholar 

  • Menge D, Makobel M, Shomari S, Tiedemann AV (2013) Development and validation of a diagrammatic scale for estimation of cashew blight for epidemiological studies. Int J Adv Res 1:26–38

    Google Scholar 

  • Michereff SJ, Maffia LA, Noronha MA (2000) Escala diagramática para avaliação da severidade da queima das folhas do inhame. Fitopatol Bras 25:612–619

    Google Scholar 

  • Newton AC, Hackett CA (1994) Subjective components of mildew assessment on spring barley. Eur J Plant Pathol 100:395–412

    Google Scholar 

  • Nita M, Ellis MA, Madden LV (2003) Reliability and accuracy of visual estimation of Phomopsis leaf blight of strawberry. Phytopathology 93(8):995–1005

    CAS  PubMed  Google Scholar 

  • Nuñez AMP, Monteiro FP, Pacheco LP, Rodríguez GAA, Nogueira CCA, Pinto FAMF, Medeiros FHV, Souza JT (2017) Development and validation of a diagrammatic scale to assess the severity of black rot of crucifers in kale. J Phytopathol 165(3):195–203

    Google Scholar 

  • Nutter FW Jr, Schultz PM (1995) Improving the accuracy and precision of disease assessments: selection of methods and use of computer-aided training programs. Can J Plant Pathol 17:174–184

    Google Scholar 

  • Nutter FW Jr, Worawitlikit O (1989) Disease. Pro: a computer program for evaluating and improving a person ability to assess disease proportion. Phytopathology 79:11–35

    Google Scholar 

  • Nutter FW Jr, Teng PS, Shokes FM (1991) Disease assessment terms and concepts. Plant Dis 75:1187–1188

    Google Scholar 

  • Nutter FW Jr, Gleason ML, Jenco JH, Christians NC (1993) Assessing the accuracy, intra-rater repeatability, and inter-rater reliability of disease assessment systems. Phytopathology 83:806–812

    Google Scholar 

  • Nutter FW Jr, Esker PD, Netto RAC (2006) Disease assessment concepts and the advancements made in improving the accuracy and precision of plant disease data. In: Savary S, Cooke BM (eds) Plant disease epidemiology: facing challenges of the 21st century. Springer, Netherlands, pp 95–103

    Google Scholar 

  • Pacheco CDA, Martelli IB, Polydoro DA, Schinor EH, Pio RM, Kupper KC, Azevedo FA (2012) Resistance and susceptibility of mandarins and their hybrids to Alternaria alternata. Sci Agric 69:386–392

    Google Scholar 

  • Parker SR, Shaw MW, Royle DJ (1995) The reliability of visual estimates of disease severity on cereal leaves. Plant Pathol 44:856–864

    Google Scholar 

  • Peever TL, Su G, Carpenter-Boggs L, Timmer LW (2004) Molecular systematics of citrus-associated Alternaria species. Mycologia 96:119–134

    CAS  PubMed  Google Scholar 

  • Perina FJ, Amaral DC, Fernandes RS, Labory CR, Teixeira GA, Alves E (2015) Thymus vulgaris essential oil and thymol against Alternaria alternata (Fr.) Keissler: effects on growth, viability, early infection and cellular mode of action. Pest Manag Sci 71:1371–1378

    CAS  PubMed  Google Scholar 

  • Renaud MSA, Amorim L, Lourenço SA, Spósito MB (2008) Diagrammatic scale for assessment of Alternaria Brown spot of citrus. Summa Phytopathol 34:270–271

    Google Scholar 

  • Rios JA, Debona D, Duarte HSS, Rodrigues FA (2013) Development and validation of a standard area diagram set to assess blast severity on wheat leaves. Eur J Plant Pathol 136:603–611

    Google Scholar 

  • Schindelin J, Arganda-Carreras I, Frise E, Kaynig V, Longair M, Pietzsch T, Preibisch S, Rueden C, Saalfeld S, Schmid B, Tinevez JY, White DJ, Hartenstein V, Eliceiri K, Tomancak P, Cardona A (2012) Fiji: an open-source platform for biological-image analysis. Nat Methods 9:676–682

    CAS  PubMed  Google Scholar 

  • Spósito MB, Amorim L, Belasque Junior J, Bassanezi RB, Aquino RD (2004) Elaboration and validation of diagrammatic scale to evaluate black spot severity in citrus fruits. Fitopatol Bras 29:81–85

    Google Scholar 

  • Stevenson M (2012) Epir: An R Package for the analysis of epidemiological data. R package version 0.9–43. http://www.R-project.org/. Accessed 24 April 2019

  • Timmer LW, Peever TL, Solel ZVI, Akimitsu K (2003) Alternaria diseases of citrus-novel pathosystems. Phytopathol Mediterr 42:99–112

    Google Scholar 

  • Tomerlin JR, Howell TA (1988) DISTRAIN: a computer program for training people to estimate disease severity on cereal leaves. Plant Dis 72:455–459

    Google Scholar 

  • Tsai HC, Yang SL, Chung KR (2013) Cyclic AMP-dependent protein kinase A negatively regulates conidia formation by the tangerine pathotype of Alternaria alternata. World J Microbiol Biotechnol 29:289–300

    CAS  PubMed  Google Scholar 

  • Vega B, Dewdney MM (2014) Distribution of QoI resistance in populations of tangerine-infecting Alternaria alternata in Florida. Plant Dis 98:67–76

    CAS  PubMed  Google Scholar 

  • Yadav NV, Vos SM, Bock CH, Wood BW (2013) Development and validation of standard area diagrams to aid assessment of pecan scab symptoms on fruit. Plant Pathol 62:325–335

    Google Scholar 

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Funding

This study was funded by Conselho Nacional de Desenvolvimento Científico e Tecnológico (grant number 475631/2013–7), Fundação de Amparo à Pesquisa do Estado de Minas Gerais (grant number CAG - APQ-02536-13), and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior.

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Authors and Affiliations

  1. Brazilian Agricultural Research Corporation (Embrapa Algodão), Campina Grande, PB, 58428-095, Brazil

    Fabiano José Perina

  2. Plant Pathology Departament, Universidade Federal de Lavras, Lavras, MG, 37200-000, Brazil

    Leônidas Leoni Belan, Silvino Intra Moreira, Eduardo Mateus Nery, Eduardo Alves & Edson Ampélio Pozza

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  1. Fabiano José Perina
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  2. Leônidas Leoni Belan
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  3. Silvino Intra Moreira
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  6. Edson Ampélio Pozza
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Correspondence to Silvino Intra Moreira.

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Perina, F.J., Belan, L.L., Moreira, S.I. et al. Diagrammatic scale for assessment of alternaria brown spot severity on tangerine leaves. J Plant Pathol 101, 981–990 (2019). https://doi.org/10.1007/s42161-019-00306-6

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