Journal of Plant Pathology

, Volume 101, Issue 2, pp 361–366 | Cite as

Isolation and characterization of Xanthomonas euvesicatoria pv. euvesicatoria causing bacterial spot in Physalis pubescens in Northeast China

  • Shuang Song
  • Yao Zhang
  • Huan Liu
  • Chun-Qing Pan
  • Ming-Xiu Yang
  • Jing-Feng Ding
  • Jun-Hua ZhangEmail author
Short Communication


Ground cherry (Physalis pubescens) is widely cultivated in Northeast China as one of its popular fruits. In the summer of 2017, suspected bacterial disease symptoms consisting of water-soaked spots and brown interveinal necrotic lesions with chlorotic margins were observed on ground cherry leaves in a field of Hailun County in Northeast China. From infected leaves, bacteria with pale-yellow, mucoid, domed circular colonies were repeatedly isolated. Based on biochemical tests and genetic characterizations of hrpB and concatenated four housekeeping genes (lepA-gyrB-gapA-gltA), the bacteria isolates obtained in this study were identified as Xanthomonas euvesicatoria pv. euvesicatoria. Pathogenicity of these isolates was confirmed on ground cherry, tomato and pepper seedlings by artificial inoculation. It was further confirmed that none of the related pathogens X. vesicatoria, X. gardneri or X. euvesicatoria pv. perforans were detected by species-specific PCR in field samples. To our knowledge, this is the first report of X. euvesicatoria pv. euvesicatoria causing bacterial spot of ground cherry in China.


Ground cherry Bacteria hrpB Multilocus sequence analysis 



This work was supported by the University Nursing Program for Young Scholars with Creative Talents in Heilongjiang Province (No. uNPYSCT-2018157), the “Young Talents” Project of Northeast Agricultural University (No. 17QC05) and the Natural Science Foundation of Heilongjiang Province, China (No. C2017032).


This study was funded by the University Nursing Program for Young Scholars with Creative Talents in Heilongjiang Province (No. uNPYSCT-2018157), the “Young Talents” Project of Northeast Agricultural University (No. 17QC05) and the Natural Science Foundation of Heilongjiang Province, China (No. C2017032).

Compliance with ethical standards

Conflict of interest

All the authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed consent

Informed consent was obtained from all individual participants included in the study.


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Copyright information

© Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2018

Authors and Affiliations

  1. 1.Department of Plant Protection, College of AgricultureNortheast Agricultural UniversityHarbinChina
  2. 2.State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant ProtectionNorthwest A&F UniversityYanglingChina

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