Isolation and characterization of Xanthomonas euvesicatoria pv. euvesicatoria causing bacterial spot in Physalis pubescens in Northeast China
- 51 Downloads
Ground cherry (Physalis pubescens) is widely cultivated in Northeast China as one of its popular fruits. In the summer of 2017, suspected bacterial disease symptoms consisting of water-soaked spots and brown interveinal necrotic lesions with chlorotic margins were observed on ground cherry leaves in a field of Hailun County in Northeast China. From infected leaves, bacteria with pale-yellow, mucoid, domed circular colonies were repeatedly isolated. Based on biochemical tests and genetic characterizations of hrpB and concatenated four housekeeping genes (lepA-gyrB-gapA-gltA), the bacteria isolates obtained in this study were identified as Xanthomonas euvesicatoria pv. euvesicatoria. Pathogenicity of these isolates was confirmed on ground cherry, tomato and pepper seedlings by artificial inoculation. It was further confirmed that none of the related pathogens X. vesicatoria, X. gardneri or X. euvesicatoria pv. perforans were detected by species-specific PCR in field samples. To our knowledge, this is the first report of X. euvesicatoria pv. euvesicatoria causing bacterial spot of ground cherry in China.
KeywordsGround cherry Bacteria hrpB Multilocus sequence analysis
This work was supported by the University Nursing Program for Young Scholars with Creative Talents in Heilongjiang Province (No. uNPYSCT-2018157), the “Young Talents” Project of Northeast Agricultural University (No. 17QC05) and the Natural Science Foundation of Heilongjiang Province, China (No. C2017032).
This study was funded by the University Nursing Program for Young Scholars with Creative Talents in Heilongjiang Province (No. uNPYSCT-2018157), the “Young Talents” Project of Northeast Agricultural University (No. 17QC05) and the Natural Science Foundation of Heilongjiang Province, China (No. C2017032).
Compliance with ethical standards
Conflict of interest
All the authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
Informed consent was obtained from all individual participants included in the study.
- Almeida NF, Yan S, Cai R, Clarke CR, Morris CE, Schaad NW, Schuenzel EL, Lacy GH, Sun X, Jones JB, Castillo JA, Bull CT, Leman S, Guttman DS, Setubal JC, Vinatzer BA (2010) PAMDB, a multilocus sequence typing and analysis database and website for plant-associated microbes. Phytopathology 100:208–215CrossRefGoogle Scholar
- Koenraadt H, van Betteray B, Germain R, Hiddink G, Jones JB, Oosterhof J, Rijlaarsdam A, Roorda P, Woudt B (2009) Development of specific primers for the molecular detection of bacterial spot of pepper and tomato. Acta Hort 808:99–102Google Scholar
- Schaad NW, Jones JB, Chun W (2001) Laboratory guide for the identification of plant pathogenic bacteria, 3rd edn. American Phytopathological Society, St PaulGoogle Scholar
- Wonni I, Cottyn B, Detemmerman S, Dao S, Ouedraogo L, Sarra S, Tekete C, Poussier S, Corral R, Triplett L, Koita O, Koebnik R, Leach J, Szurek B, Maes M, Verdier V (2014) Analysis of Xanthomonas oryzae pv. oryzicola population in Mali and Burkina Faso reveals a high level of genetic and pathogenic diversity. Phytopathology 104:520–531CrossRefGoogle Scholar