Skip to main content
Log in

Diaporthe species complex occurring on Asparagus kiusianus in Japan

  • Short Communication
  • Published:
Journal of Plant Pathology Aims and scope Submit manuscript

Abstract

In 2016, a new disease caused by the Diaporthe species complex was discovered in “hamatamabouki”, the Japanese wild asparagus (Asparagus kiusianus Makino), in the coastal area of Northern Kyushu. Two distinct fungal species were recorded as causing the brown and oval-shaped lesions shown by the aerial parts of the plants. Diaporthe asparagi (Phomopsis asparagi) was identified based on molecular analysis of the nuclear ribosomal internal transcribed spacers (ITS), while D. unshiuensis, a first report on the genus Asparagus, was identified using the multi-locus sequence analysis of the ITS region, the translation elongation factor 1-alpha (TEF1-alpha) and the beta-tubulin (TUB) genes. The morphological characteristics of these species were observed, most of which being in accordance with previous descriptions. When A. kiusianus plants were exposed to infection by D. asparagi and D. unshiuensis to fulfill Koch’s postulates, both fungal species proved to be pathogenic.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3

References

  • Abdelrahman M., Suzumura N., Mitoma M., Matsuo S., Ikeuchi T., Mori M., Murakami K., Ozaki Y., Matsumoto M., Uragami A., Kanno A., 2017. Comparative de novo transcriptome profiles in Asparagus officinalis and A. kiusianus during the early stage of Phomopsis asparagi infection. Sci Rep 7:2608

  • Bubak F., 1906. Zweiter Beitrag zur Pilzflora von Montenegro. Bulletin de l’Herbier Boissier 6:393–408

  • Carbone I, Kohn L (1999) A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia 91:553–556

    Article  CAS  Google Scholar 

  • Davis RD (2001) Asparagus stem blight recorded in Australia. Australas Plant Pathol 30:181–182

    Article  Google Scholar 

  • Elena K (2006) First report of Phomopsis asparagi causing stem blight of asparagus in Greece. Plant Pathol 55:300

    Article  Google Scholar 

  • Glass NL, Donaldson GC (1995) Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl Environ Microbiol 61:1323–1330

    CAS  PubMed  PubMed Central  Google Scholar 

  • Huang F, Udayanga D, Wang X, Hou X, Mei X, Fu Y, Hyde KD, Li H (2015) Endophytic Diaporthe associated with Citrus: a phylogenetic reassessment with seven new species from China. Fugal Biology 119:331–347

    Google Scholar 

  • Huelsenbeck JP, Ronquist F (2001) MrBayes: Bayesian inference of phylogenetic trees. Bioinformatics 17:754–755

    Article  CAS  Google Scholar 

  • Ito T, Konno I, Kubota S, Ochiai T, Sonoda T, Hayashi Y, Fukuda T, Yokoyama J, Nakayama H, Kameya T, Kanno A (2011) Production and characterization of interspecific hybrids between Asparagus kiusianus Makino and A. officinalis L. Euphytica 182:285–294

    Article  Google Scholar 

  • Iwato M, Kosaza M, Takeuchi Y, Matsumoto M, Inada M, Ozaki Y, Okubo H (2014) Stem blight resistance of Asparagus kiusianus and its hybrid with A. officinalis. Adv Hortic Sci 28:202–207

    Google Scholar 

  • Kubota S, Konno I, Kanno A (2012) Molecular phylogeny of the genus Asparagus (Asparagaceae) explains interspecific crossability between garden asparagus (A. officinalis) and other Asparagus species. Theor Appl Genet 124:345–354

    Article  PubMed  Google Scholar 

  • Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874

    Article  CAS  PubMed  Google Scholar 

  • Narayanasamy P (1997) Plant pathogen detection and disease diagnosis. Marcel Dekker, New York, NY, USA

    Google Scholar 

  • Nylander JAA (2004) MrModeltest v2. Program distributed by the author. Uppsala University, Uppsala, Sweden, Evolutionary Biology Centre

    Google Scholar 

  • Punithalingam E (1990) CMI descriptions of fungi and bacteria. Mycopathologia 112:39–40

    Article  Google Scholar 

  • Saccardo PA (1878) Fungi Veneti novi vel critici vel Mycologiae Venetae addendi. Michelia 1:239–275

    Google Scholar 

  • Santos JM, Phillips AJL (2009) Resolving the complex of Diaporthe (Phomopsis) species occurring on Foeniculum vulgare in Portugal. Fungal Divers 34:111–125

    Google Scholar 

  • Udayanga D, Castlebury LA, Rossman AY, Hyde KD (2014) Species limits in Diaporthe: molecular re-assessment of D. citri, D. cytosporella, D. foeniculina and D. rudis. Persoonia 32:83–101

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Uecker FA, Johnson DA (1991) Morphology and taxonomy of species of Phomopsis on Asparagus. Mycologia 83:192–199

    Article  Google Scholar 

  • White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic Press, San Diego, CA, USA, pp 315–322

    Google Scholar 

  • Yang YQ, Li XM, Meng F, Lan B, Zhang J (2012) Identification of the pathogen of asparagus stem blight and analysis of its regional difference. Journal of Plant Protection 39:315–320

    CAS  Google Scholar 

  • Zaw M, Naing TAA, Matsumoto M (2017) First report of stem blight of asparagus caused by Phomopsis asparagi in Myanmar. New Disease Reports 35:17

    Article  Google Scholar 

Download references

Acknowledgements

This research was supported by a grant from the Agriculture, Forestry and Fisheries Research Council of Japan (27002B).

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Thao Le Dinh.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Le Dinh, T., Zaw, M. & Matsumoto, M. Diaporthe species complex occurring on Asparagus kiusianus in Japan. J Plant Pathol 101, 161–167 (2019). https://doi.org/10.1007/s42161-018-0140-9

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s42161-018-0140-9

Keywords

Navigation