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Antibacterial activity and mechanism of ThDP analogs against rice brown stripe pathogen Acidovorax avenae subsp. avenae RS-1

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Abstract

Thiamin diphosphate (ThDP) analogs have been designed and synthesized based on the ThDP binding site of the pyruvate dehydrogenase multienzyme complex E1 of Escherichia coli. This study investigated the effect of 66 novel ThDP analogs on rice bacterial brown stripe pathogen Acidovorax avenae subsp. avenae strain RS-1. Results indicated that three of the 66 ThDP analogs (designated as 20, 21 and 53) significantly inhibited the in vitro growth of strain RS-1. However, no obvious cell lysis and destruction was found for this rice pathogenic bacterium, which were supported by morphological evidence of transmission electron microscope. In contrast, the three ThDP analogs significantly reduced biofilm formation and the activity of the pyruvate dehydrogenase of strain RS-1. Furthermore, the differential expression of ThDP target gene and 20 secretion system related genes were revealed by using quantitative real-time PCR. Among these, the expressions of ThDP target gene and VgrG-5 under the treatment of ThDP 20 were strongly induced compared to the control, which indicated that the antibacterial mechanism of ThDP analogs may be mainly due to the changed expression of ThDP target gene and secretion system related genes rather than causing damage to cell membrane. Taken together, the application of synthesized ThDP analogs might be a tractable strategy to overcome the pathogen of rice bacterial brown stripe.

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Acknowledgments

This work was supported by National Natural Science Foundation of China (31571971, 31371904, 21472062), Zhejiang Provincial Project (2017C02002), National Key Research and Development Program of China (2017YFD0201104), Shanghai Agricultural Basic Research Project (2014:7-3-1), the Fundamental Research Funds for the Central Universities, the Agricultural Ministry of China (nyhyzx 201303015), National Basic Research Program of China (No. 2010CB126100), Dabeinong Funds for Discipline Development and Talent Training in Zhejiang University, Key Subject Construction Program of Zhejiang for Modern Agricultural Biotechnology and Crop Disease Control (2010DS700124- KF1710), China Postdoctoral Science Foundation (Grant No. 2017 M612003).

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Author notes

  1. Xiao Xuan Wang and Hang Ying Qi contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory of Rice Biology, Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou, 310058, China

    Xiao Xuan Wang, Jie Chen, Ying Zi Yang, Wen Qiu & Bin Li

  2. Zhuji Extension and Service Center of Agriculture Technical, Zhuji, 310020, China

    Hang Ying Qi

  3. Key Laboratory of Pesticide & Chemical Biology (CCNU), Ministry of Education; College of Chemistry, Central China Normal University, Wuhan, 430079, China

    Wei Wang, Peng Zou & Hong Wu He

  4. State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China

    Yan Li Wang & Guo Chang Sun

Authors
  1. Xiao Xuan Wang
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  2. Hang Ying Qi
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  3. Jie Chen
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  4. Ying Zi Yang
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  5. Wen Qiu
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  6. Wei Wang
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  8. Bin Li
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  9. Yan Li Wang
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  10. Hong Wu He
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  11. Guo Chang Sun
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Correspondence to Wen Qiu or Guo Chang Sun.

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Wang, X.X., Qi, H.Y., Chen, J. et al. Antibacterial activity and mechanism of ThDP analogs against rice brown stripe pathogen Acidovorax avenae subsp. avenae RS-1. J Plant Pathol 101, 59–69 (2019). https://doi.org/10.1007/s42161-018-0137-4

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  • DOI: https://doi.org/10.1007/s42161-018-0137-4

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