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First report of natural occurrence of groundnut bud necrosis virus in Solanum torvum Sw. in India

  • Shweta Kumari
  • Krishnan Nagendran
  • Vikas Dubey
  • Chinnappa Manimurugan
  • Achuit Kumar Singh
  • Awadhesh Bahadur Rai
  • Bijendra Singh
Disease Note

Solanum torvum Sw., commonly known as turkey berry, belongs to the family Solanaceae. It is extensively used for medicinal purposes (Ndebia et al. 2007). Foliar necrotic and chlorotic ring spots were observed on 10–15% of S. torvum plants at the IIVR research farm, Varanasi (Uttar Pradesh, India) in December 2015. Enveloped spherical shaped virions 80–120 nm in size were observed by transmission electron microscopy in tissue from symptomatic plants. Five symptomatic plants tested were positive for groundnut bud necrosis virus (GBNV) from the genus Orthotospovirus, family Tospoviridae in Direct Antigen Coating-Enzyme Linked Immunosorbent Assay (DAC-ELISA) with the antibodies obtained from the International Crops Research Institute for the Semi-Arid Tropics, Hyderabad, India. To confirm the presence of GBNV, total RNA was extracted from five symptomatic plants along with asymptomatic samples using TRIzol reagent (Ambion™) and subjected to reverse transcription polymerase chain reaction (RT-PCR) using a degenerate primer pair to amplify a portion of the L RNA segment of orthotospoviruses (Chu et al. 2001). Amplicons of 800 bp (GenBank accession No. KY940037) from symptomatic plants were cloned and sequenced. The nucleotide sequence had 98% identity with the L RNA of GBNV from Indonesia (FJ177300). Additionally, a 830 bp fragment was amplified by RT-PCR with specific primers to the coat protein (CP) gene of GBNV (Nagendran et al. 2018). The CP gene nucleotide sequence (MG913145) had 98% identity with the CP gene of GBNV from eggplant in India (GQ844886). This is the first report of GBNV in S. torvum in India.

References

  1. Chu F-H, Chao C-H, Chung M-H, Chen C-C, Yeh S-D (2001) Completion of the genome sequence of Watermelon silver mottlevirus and utilization of degenerate primers for detecting tospoviruses in five serogroups. Phytopathology 91:361–368CrossRefPubMedGoogle Scholar
  2. Nagendran K, Kumari S, Rai AB, Manimurugan C, Singh B, Karthikeyan G, Naidu RA (2018) First report of peanut bud necrosis virus infecting bitter gourd (Momordica charantia L.) in India. Plant Dis 102(3):690CrossRefGoogle Scholar
  3. Ndebia EJ, Kamgang R, Nkeh-ChungagAnye BN (2007) Analgesic and anti-inflammatory properties of aqueous extract from leaves of Solanum Torvum (Solanaceae). Afr J Tradit Complement Altern Med 4:240–244Google Scholar

Copyright information

© Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2018

Authors and Affiliations

  1. 1.Division of Crop ProtectionICAR-Indian Institute of Vegetable ResearchVaranasiIndia
  2. 2.Division of Crop ImprovementICAR-Indian Institute of Vegetable ResearchVaranasiIndia

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