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First identification and characterization of cucumber mosaic virus from Corchorus olitorius in Japan

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Abstract

CMV infected in Corchorus olitorius plants cultivated on a farm in Miyagi Prefecture, Japan, was analyzed via western blotting using antibody against the coat protein of cucumber mosaic virus (CMV). The virus was purified from Nicotiana benthamiana leaves rub-inoculated with a homogenate of infected C. olitorius containing CMV genomic RNA. The CMV isolate was named CMV-Co-46. Nucleotide sequence analysis of RNA1, 2, and 3 cDNA grouped CMV-Co-46 in subgroup IA. Inoculation of C. olitorius cotyledons with CMV-Co-46 resulted in slight yellowing on primary and secondary leaves seven days after inoculation and severely suppressing plant growth. However, no further systemic spread was observed and the infected plants were able to recover from the stunting symptoms. These findings suggest that restricted infection followed by recovery from systemic stunting may be a unique feature of the C. olitorius response to CMV. Management of CMV infection at an early growth stage is therefore important in obtaining healthy nursery plants of C. olitorius.

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References

  • Biswas C, Dey P, Mitra S, Bera A, Satpathy S, Karmakar PG (2014) First report of potato leaf roll virus (PLRV) naturally occurring on jute (Corchorus olitorius) in India. Plant Dis 98:1592

    Article  PubMed  Google Scholar 

  • Cillo F, Roberts IM, Palukaitis P (2002) In situ localization and tissue distribution of the replication-associated proteins of cucumber mosaic virus in tobacco and cucumber. J Virol 76:10654–10664

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Ding B, Li Q, Nguyen L, Palukaitis P, Lucas WJ (1995) Cucumber mosaic virus 3a protein potentiates cell-to-cell trafficking of CMV RNA in tobacco plants. Virology 207:345–353

    Article  CAS  PubMed  Google Scholar 

  • Edwards MC, Gonsalves D (1983) Grouping of seven biological defined isolates of cucumber mosaic virus by peptide mapping. Phytopathology 73:1117–1120

    Article  CAS  Google Scholar 

  • Garcia-Arenal F, Palukaitis P (2008) Cucumber mosaic virus. In: Mahy BWJ, van Regenmortel MHV (eds) Encyclopedia of Virology, 3rd edn. Elsevier- Academic Press, San Diego, pp 614–619

    Chapter  Google Scholar 

  • Ghosh SK, Som D (1998) Diseases of jute and their control. In: Khurana SMP (ed) Pathological problems of economic crop plants and their management. Scientific Publishers (India), Jodhpur, pp 329–344

    Google Scholar 

  • Ghosh RS, Paul AS, Das AP, Palit AS, Acharyya AA, Das AJI, Mir A, Ghosh SK, Roy A (2008) Molecular evidence for existence of a new world begomovirus associated with yellow mosaic disease of Corchorus capsularis in India. Aust Plant Dis Notes 3:59–62

    Article  CAS  Google Scholar 

  • Ghosh RS, Palit AP, Paul S, Ghosh SK, Roy A (2012) Detection of Corchorus golden mosaic virus associated with yellow mosaic disease of jute (Corchorus capsularis). Indian J Virol 23:70–74

    Article  PubMed  PubMed Central  Google Scholar 

  • Guo HS, Ding SW (2002) A viral protein inhibits the long range signaling activity of the gene silencing signal. EMBO J 21:398–407

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Ha C, Coombs S, Revill P, Harding R, Vu M, Dale J (2006) Corchorus yellow vein virus, a new world geminivirus from the old world. J Gen Virol 87:997–1003

    Article  CAS  PubMed  Google Scholar 

  • Hasan MM, Meah MB, Ali MA, Okazaki K, Sano Y (2015) Characterization and confirmation of corchorus golden mosaic virus associated with jute in Bangladesh. J Plant Pathol Microbiol 6:256

    Google Scholar 

  • Hayes RJ, Buck KW (1990) Complete replication of a eukaryotic virus RNA in vitro by a purified RNA-dependent RNA polymerase. Cell 63:363–368

    Article  CAS  PubMed  Google Scholar 

  • Owen J, Palukaitis P (1988) Characterization of cucumber mosaic virus. I. Molecular heterogeneity mapping of RNA 3 in eight CMV strains. Virology 69:496–502

    Google Scholar 

  • Palukaitis P, García-Arenal F (2003) Cucumoviruses. Adv Virus Res 62:241–323

    Article  CAS  PubMed  Google Scholar 

  • Palukaitis P, Roossinck MJ, Dietzgen RG, Francki RIB (1992) Cucumber mosaic virus. Adv Virus Res 41:281–348

    Article  CAS  PubMed  Google Scholar 

  • Roossinck MJ, Zhang L, Hellwald KH (1999) Rearrangements in the 59 nontranslated region and phylogenetic analyses of cucumber mosaic virus RNA3 indicate radial evolution of three subgroups. J Virol 73:6752–6758

    CAS  PubMed  PubMed Central  Google Scholar 

  • Sambrook J, Russell DW (2001) Molecular Cloning: A Laboratory Manual, 3rd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor

    Google Scholar 

  • Schwinghamer MW, Symons RH (1975) Fractionation of cucumber mosaic virus RNA and its translation in a wheat embryo cell-free system. Virology 63:252–262

    Article  CAS  PubMed  Google Scholar 

  • Suzuki M, Kuwata S, Kataoka J, Masuta C, Nitta N, Takanami Y (1991) Functional analysis of deletion mutation of cucumber mosaic virus RNA3 using as in vitro transcriptions system. Virology 183:108–113

    Article  Google Scholar 

  • Takahashi H, Ehara Y (1993) Severe chlorotic spot symptoms in cucumber mosaic virus strain Y-infected tobaccos are induced by a combination of the virus coat protein gene and two host recessive genes. Mol Plant-Microbe Interact 6:182–189

    Article  CAS  PubMed  Google Scholar 

  • Takahashi H, Goto N, Ehara Y (1994) Hypersensitive response in cucumber mosaic virus-inoculated Arabidopsis thaliana. Plant J 6:369–377

    Article  Google Scholar 

  • Takahashi H, Suzuki M, Natsuaki K, Shigyo T, Hino K, Teraoka T, Hosokawa D, Ehara Y (2001) Mapping the virus and host genes involved in the resistance response in cucumber mosaic virus-infected Arabidopsis thaliana. Plant Cell Physiol 42:340–347

    Article  CAS  PubMed  Google Scholar 

  • Tulio AZ Jr, Ose K, Chachin K, Ueda Y (2002) Effects of storage temperatures on the postharvest quality of jute leaves (Corchorus olitorius L.). Postharvest Biol Technol 26:329–338

    Article  CAS  Google Scholar 

  • Wahyuni WS, Dietzgen RG, Hanada K, Francki RIB (1992) Serological and biological variation between and within subgroup I and II strains of cucumber mosaic virus. J Plant Pathol 41:282–297

    Article  CAS  Google Scholar 

  • Yokoyama S, Hiramoto K, Fujikawa T, Kondo H, Konishi N, Sudo S, Iwashima M, Ooi K (2014) Topical application of Corchorus olitorius leaf extract ameliorates atopic dermatitis in NC/Nga mice. Dermatology Aspects. Journal 2:3. https://doi.org/10.7243/2053-5309-2-3.

    Article  Google Scholar 

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Acknowledgements

This study was financially supported by grants for “Scientific Research on Innovative Areas” from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan (Grant numbers: 16H06429, 16 K21723, and 16H06435), and by the Japan Society for the Promotion of Science (JSPS) through the JSPS Core-to-Core Program (Advanced Research Networks) entitled “Establishment of international agricultural immunology research-core for a quantum improvement in food safety”. This work was also supported by the Egyptian Government.

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Authors and Affiliations

  1. Graduate School of Agricultural Science, Tohoku University, 468-1 Aramaki-Aza-Aoba, Sendai, 980-0845, Japan

    Sara E. Hanbal, Shuhei Miyashita, Sugihiro Ando & Hideki Takahashi

  2. Department of Virus & Phytoplasma, Agriculture Research Center, Plant Pathology Research Institute, Giza, 12619, Egypt

    Sara E. Hanbal

  3. Department of Agricultural Botany, Faculty of Agriculture, Kafrelsheikh University, P.O. Box 33516, Kafr El Sheikh, Egypt

    Samir A. Sidaros

Authors
  1. Sara E. Hanbal
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  2. Shuhei Miyashita
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  3. Sugihiro Ando
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  4. Samir A. Sidaros
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  5. Hideki Takahashi
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Corresponding author

Correspondence to Hideki Takahashi.

Electronic supplementary material

Tables S1

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Table S2

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Table S3

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Fig. S1

Survey of CMV-infected Corchorus olitorius plants cultivated on a farm in Ohsato City, Miyagi Prefecture, Japan. Infection of 47 independent plants with CMV was screened by immunological detection of the coat protein of CMV by western blot. The band pattern of Plant no (#) 1 to 12, no (#) 13 to 23, no (#) 24 to 35, and no (#) 36 to 47, were shown in A, B, C and D, respectively. Molecular marker (Marker) and a sample mock-inoculated healthy C. olitorius plants as negative control (Mock), were loaded on left lanes of each gel for western blot. (JPG 68 kb)

Fig. S2

Symptoms in non-inoculated upper leaves of CMV-infected Corchorus olitorius 14 days after inoculation and immunological detection of the coat protein (CP) in all leaves. Primary, second, third, fourth, and fifth leaves 14 days after CMV-Co-46 or CMV-Y inoculation and mock-inoculation (upper panel). Immunoblotting analysis of CP in each leaf 14 days after CMV-Co-46-, CMV-Y-, and mock-inoculation (lower panel). Cotyledons had dropped by this time. (JPG 87 kb)

Fig. S3

Immunological detection of the coat protein (CP) in CMV-Co-46-, CMV-Y- and mock-inoculated fourth leaves by western blotting. As an internal protein control, the RuBiSCO band was visualized by staining with Coomassie Brilliant Blue R-250. (JPG 32 kb)

Fig. S4

Systemic symptoms in Corchorus olitorius plants 14 days after inoculation of cotyledons with CMV-Co-47 (upper panel) and CMV-Y (lower panel). As a control, mock-inoculated cotyledons are also shown. (JPG 76 kb)

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Hanbal, S.E., Miyashita, S., Ando, S. et al. First identification and characterization of cucumber mosaic virus from Corchorus olitorius in Japan. J Plant Pathol 100, 561–565 (2018). https://doi.org/10.1007/s42161-018-0099-6

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