First report of cucumber mosaic virus infecting Arachis hypogaea in Jiangxi Province in China

  • Feng Zhu
  • Yan Ping Che
  • Yang Kai Zhou
  • Shi Chang Cheng
  • Zheng Liu
  • Zhao Lin Ji
Disease Note
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Foliar virus-like symptoms, including mosaic and yellowing mosaic were observed on peanut, Arachis hypogaea, in Pengze county, Jiangxi Province, in eastern China in July 2017. Leaf samples were collected from five symptomatic peanut plants. All samples were assayed by western blotting using antisera to cucumber mosaic virus (CMV) (Zhu et al. 2013). Results revealed that three of the five samples were positive for CMV. To confirm the presence of CMV, RT-PCR was performed using primers CMV-CP-F (5′-TGTAGGGAGTGAACGCTGGAA-3′) and CMV-CP-R (5′-GATGGCGGCAACGGATAA-3′), which were designed to amplify a partial fragment of the coat protein gene. The expected 244 bp amplicon was amplified from the three symptomatic leaf samples that were positive for CMV in western blotting assay. DNA products were purified, cloned into the pMD19-T vector (TaKaRa) and sequenced (Zhu et al. 2017). Results showed that the three CMV sequences were identical. Therefore, only one of these sequences was submitted to GenBank (accession No. MG564343). Nucleotide BLAST analysis revealed that the sequence showed 99% nucleotide identity with CMV isolate PR36 (M98500). The virus was mechanically transmitted by inoculating sap from symptomatic peanut leaves to Nicotiana tabacum, which showed mosaic symptoms on uninoculated leaves that tested positive for CMV by RT-PCR with the aforementioned primers. To our knowledge, this is the first report of CMV in peanut in Jiangxi Province in China.

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (31500209), Natural Science Foundation of the Higher Education Institutions of Jiangsu Province of China (15KJB210007) and earmarked fund for Modern Agro-industry Technology Research System (CARS-31-2-02).

References

  1. Zhu F, Zhang P, Meng YF, Xu F, Zhang DW, Cheng J, Lin HH, Xi DH (2013) Alpha-momorcharin, a RIP produced by bitter melon, enhances defense response in tobacco plants against diverse plant viruses and shows antifungal activity in vitro. Planta 237:77–88CrossRefPubMedGoogle Scholar
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Copyright information

© Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2018

Authors and Affiliations

  • Feng Zhu
    • 1
  • Yan Ping Che
    • 1
  • Yang Kai Zhou
    • 1
  • Shi Chang Cheng
    • 1
  • Zheng Liu
    • 1
  • Zhao Lin Ji
    • 1
  1. 1.College of Horticulture and Plant ProtectionYangzhou UniversityYangzhouChina

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