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Trichoderma elicitors create a potential chemical barrier through induced systemic resistance against Colletotrichum falcatum and minimise sucrose losses in sugarcane

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Abstract

Red rot disease caused by Colletotrichum falcatum leads to acute sucrose losses in sugarcane. Minimising sucrose losses through Trichoderma elicitors will fetch an eco-friendly technology to sugarcane growers. An experiment was conducted for evaluating the effects of elicitors from Trichoderma strains (STr 83, STr 108 and STr 116) against Colletotrichum falcatum for minimising sucrose losses. Following Trichoderma elicitor perception, a chemical barrier against C. falcatum was activated in sugarcane through enhanced accumulation of phenols, enhanced polyphenol oxidase, peroxidase, superoxidase dismutase, phenylalanine ammonia lyase, tyrosine ammonia lyase, chitinase and β-1,3 glucanase activities arrayed in setts at 45 days after pathogen inoculation (dpi). Phenol fluxes and enhanced activities of polyphenol oxidase, peroxidase, superoxidase dismutase, phenylalanine ammonia lyase, tyrosine ammonia lyase, chitinase and β-1,3 glucanase in foliar tissues were carried forward till 330 dpi (harvest stage). STr 83 elicitors exhibited maximum increase in phenols (106%), chitinase (540%), 1, 3 β-glucanases (160%) and activities of polyphenol oxidase (125%), peroxidase (346%), superoxide dismutase (54%), phenylalanine ammonia lyase (162%) and tyrosine ammonia lyase (206%) at 210 dpi. This chemical barrier orchestrated the diverse biochemical pathways for induced systemic resistance and minimised sucrose losses by 0.19–0.26 t ha−1 at cane harvest stage.

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Acknowledgements

We thank Director, ICAR-IISR, Lucknow, for his constant support and encouragement. Financial support under UGC Junior Research Fellowship (JRF) scheme, University Grants Commission (UGC), New Delhi, India, is gratefully acknowledged. We extend our sincere thanks to Mr. Abhilash Kumar Shukla for providing the weather data. The help provided by Mr. Chatrpal, Mr. Santosh, Mr. Surender and Mr. Raj Kumar during the field experiment and laboratory studies is also duly acknowledged.

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Authors and Affiliations

  1. ICAR- Indian Institute of Sugarcane Research, Lucknow, Uttar Pradesh, 226002, India

    Pushpa Singh, Nidhi Tripathi, Deeksha Joshi & Ashwini Dutt Pathak

  2. Department of Chemistry, University of Lucknow, Lucknow, Uttar Pradesh, 226007, India

    Arun Sethi

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  1. Pushpa Singh
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  2. Nidhi Tripathi
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  3. Deeksha Joshi
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  4. Ashwini Dutt Pathak
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  5. Arun Sethi
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Correspondence to Pushpa Singh.

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Singh, P., Tripathi, N., Joshi, D. et al. Trichoderma elicitors create a potential chemical barrier through induced systemic resistance against Colletotrichum falcatum and minimise sucrose losses in sugarcane. J Plant Pathol 100, 151–162 (2018). https://doi.org/10.1007/s42161-018-0034-x

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  • DOI: https://doi.org/10.1007/s42161-018-0034-x

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