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Incidence and genetic diversity of apple chlorotic leaf spot virus in Iran

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Abstract

Apple chlorotic leaf spot virus (ACLSV) is one of the most common viruses infecting apple and pear trees, and Iran is among the top ten apple-producing countries in the world. In the present study, incidence and genetic diversity of ACLSV were investigated in the main apple-growing regions of Iran. To achieve this purpose, a total of 1053 leaf samples were collected from orchards located in nine Iranian provinces. ACLSV infection was detected by DAS-ELISA in 48 samples (4.55%) from seven provinces and was confirmed by RT-PCR. Based on the geographical origin, 19 representative isolates were selected for phylogenetic analysis. Nineteen amplicons, with a size of 677 base pairs (bp), containing the 3′ end of the movement protein (MP), the coat protein (CP) gene, and 3′-UTR sequence, were sequenced. Sequence analysis, using data of 45 isolates from 12 different countries, including the 19 Iranian isolates, showed that CP gene among the Iranian isolates were 80–99% identical at both nucleotide and amino acid levels, and these isolates were placed in the B6 (AB326224) and P-205 (D14996) ACLSV groups. This is the first genetic analysis of ACLSV in Iran.

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Acknowledgements

We would like to thank the Iranian Ministry of Science, Research and Technology, for supporting F. Abtahi’s internship in Italy.

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Authors and Affiliations

  1. Plant Pathology Department, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran

    Faezehossadat Abtahi, Masoud Shams-Bakhsh, Naser Safaie & Abdolbaset Azizi

  2. Department of Agricultural Sciences, Plant Pathology and Plant Protection Section, University of Bologna, Viale Fanin, 40, Bologna, Italy

    Concepcion Rubies Autonell & Claudio Ratti

Authors
  1. Faezehossadat Abtahi
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  2. Masoud Shams-Bakhsh
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  3. Naser Safaie
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  4. Abdolbaset Azizi
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  5. Concepcion Rubies Autonell
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  6. Claudio Ratti
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Corresponding author

Correspondence to Masoud Shams-Bakhsh.

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Abtahi, F., Shams-Bakhsh, M., Safaie, N. et al. Incidence and genetic diversity of apple chlorotic leaf spot virus in Iran. J Plant Pathol 101, 513–519 (2019). https://doi.org/10.1007/s42161-018-00224-z

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