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Feature of blast resistant near-isogenic lines using an elite maintainer line II-32B by marker-assisted selection

Journal of Plant Pathology volume 101, pages 491–501 (2019)Cite this article

Abstract

Rice plays a crucial role in food production worldwide as one of the most important cereal crops. Breeding blast disease resistant varieties is a long-term aim for sustainable rice production. II-32B is an elite maintainer line of II-32A which has been widely used as a hybrid rice parent in China. However, it is significantly susceptible to rice blast. In this study, multiple alleles, Pi-2 and Pi-9, and the chromosomal segment of blast resistance genes, Pi-1 and Pi-kh, were introgressed to receptor parents II-32B by marker-assisted backcross breeding. Eleven near-isogenic lines (NILs) with single Pi-gene and 12 pyramid lines containing double or three homozygous resistance genes were obtained. These improved lines were identified by inoculating Magnaporthe oryzae isolates in the field/greenhouses; the result showed that the resistance of pyramid lines containing three resistance genes was stronger than the lines with double or single gene, whereas the lines with double genes were stronger than the one with single-gene. Additionally, pyramiding of different Pi-genes was effective in obtaining the durable blast resistance in rice. This implies that gene pyramiding in rice can increase spectrum and strength of resistance to blast. Furthermore, three-line retention of sixteen improved NILs was kept well and the agronomic traits were consistence with II-32B. We conclude that gene pyramiding is an effective breeding approach for the improvement of rice varieties with durable resistance to blast in the future.

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Acknowledgements

This work was supported by National Natural Science Foundation of China (U13052112) and Natural Science Foundation of Fujian Province (K53120003).

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Authors and Affiliations

  1. Key Laboratory of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education, Fuzhou, 350002, China

    Huazhong Guan, Yunlin Jiang, Damei Mao, Runsen Pan, Yuanchang Zhou & Zhiwei Chen

  2. Fujian Provincial key laboratory of crop breeding by design, Fujian Agricultural & Forestry University, Fuzhou, 350002, China

    Huazhong Guan, Xinpo Hou, Yunlin Jiang, Damei Mao, Runsen Pan, Yuanchang Zhou & Zhiwei Chen

  3. Department of Crop, Soil & Environmental Sciences, and Department of Horticulture, University of Arkansas, Fayetteville, AR, 72701, USA

    Vibha Srivastava

  4. The Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agricultural & Forestry University, Fuzhou, 350002, China

    Meilian Chen & Zonghua Wang

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  1. Huazhong Guan
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Correspondence to Zhiwei Chen.

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Guan, H., Hou, X., Jiang, Y. et al. Feature of blast resistant near-isogenic lines using an elite maintainer line II-32B by marker-assisted selection. J Plant Pathol 101, 491–501 (2019). https://doi.org/10.1007/s42161-018-00222-1

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Keywords

  • Gene pyramiding
  • Marked-assisted selection
  • Oryza sativa L.
  • Resistance to rice blast
  • Near-isogenic lines