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Continuous preparation for rifampicin


To reduce the cost and improve the efficiency for rifampicin preparation, a continuous flow synthesis of rifampicin starting from rifamycin S and tert-butylamine was studied in a microreactor. Two reaction steps and one purification step were coupled in a microreactor, and rifampicin was obtained with 67% overall yield. This method used 25% less 1-amino-4-methyl piperazine and got 16% higher overall yield without changing solvent and purification process between steps. This method has a good potential for further industrial application.

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Authors acknowledges the funding from The jiangsu synergetic innovation center for advanced bio-manufacture (XTE1852), The National Key Research and Development Program of China (2016YFB0301501), National Natural Science Foundation of China (21522604), National Natural Science Foundation of China (U1463201), The National Natural Science Foundation of China (21776130), The jiangsu synergetic innovation center for advanced bio-manufacture (XTE1821), The jiangsu synergetic innovation center for advanced bio-manufacture (XTE1802).

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Correspondence to Kai Guo.

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Li, X., Liu, Z., Qi, H. et al. Continuous preparation for rifampicin. J Flow Chem 8, 129–138 (2018).

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  • Continuous flow synthesis
  • Coupling of reaction and separation
  • Rifampicin preparation