Etidronic acid and its calcium-comprising complexes in the treatment of experimental osteoporosis in estrogen-deficient rats
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The aim of this study was to investigate the composition, stability, and biological activity of the complexes formed in solution upon interaction of calcium (Ca) ions with etidronic acid (EA), and to compare their effects on bone mineral density (BMD) in estrogen-deficient rats with osteoporosis.
Materials and methods
The study was conducted on 40 female Wistar rats. Osteoporosis was modeled by bilateral oophorectomy and therapy was started 4 months after surgery. Four groups were under study: the first group did not receive any osteoporotic treatment, only supplementation of Ca/vitamin D (D) in their diet. The second group received a single parenteral dose of EA with Ca/D supplementation. The third group received a Ca complex of EA (Ca-EA) without additional use of Ca/D. The fourth group received Ca-EA with Ca/D. Bone parameters (BMD and bone mineral content [BMC] in spine and total body) were measured by X‑ray densitometry using Prodigy (GE Medical systems, Model 8743, 2005; USA; program “Experimental animals”) thrice (before treatment, after 1 and 3 months of follow-up).
Therapy with EA and Ca/D showed significant differences in total body BMD (tbBMD) only after 3 months. We established reliable changes in spine BMD (sBMD) and tbBMD after 1 and 3 months of treatment in group 3 rats: administration of Ca-EA with Ca/D had positive effects on sBMD at 1 and 3 months, and on tbBMD after 3 months. We did not establish any significant changes between sBMD and tbBMD after 1 and 3 months of therapy dependent on additional Ca/D supplementation (groups 3 and 4).
We showed a positive effect of the studied Ca-EA complexes on the BMD and BMC indices, with or without additional supplementation of Ca/D. More explicit dynamics of indices were observed in the group of animals treated with Ca-EA without additional Ca/D after 1 and 3 months of follow-up.
KeywordsOophorectomy Rats, Wistar Calcium-comprising complexes of etidronic acid Bone mineral density
We are grateful for the collaboration of the group of scientists of D. F. Chebotarev Institute of Gerontology of NAMS Ukraine (Kyiv, Ukraine) who performed bilateral oophorectomy and DXA measurement.
Compliance with ethical guidelines
Conflict of interest
V.V. Povoroznyuk, N.V. Grygorieva, V.I. Pekhnyo, H. Resch, O.M. Kozachkova, and N.V. Tsaryk declare that they have no competing interests.
All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.
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