The Genetic Variability (RAPD) and Genotoxicity In Vivo (Alkaline and Fpg-Modified Comet Assay) in Chub (Squalius cephalus): The Sava River Case Study

Abstract

The major objective of this study was to investigate the impact of wastewaters on the level of genotoxicity and the possible reflection of genotoxicity on the genetic variability in chub (Squalius cephalus) collected from the sites under different pollution pressures along the Sava River. To emphasize the impact of wastewaters, this study was carried out in a season with low water discharge. The level of genotoxic potential at the sites was studied by alkaline and Fpg-modified comet assay in fish blood, while RAPD technique was employed on the fish muscle tissue. The variations in the genotoxic potential were observed along the Sava River based on the level of DNA damage in blood of the specimens collected at the selected sites. Clustering of the impacted sites was also observed based on RAPD fingerprints. Comparison of the data obtained in RAPD and the data from the comet assay indicated the possible reflection of genotoxicity on the genetic variability in chub collected from the sites with different pollution pressure. This is a preliminary study which reports the differentiation of the population of chub along the Sava River based on RAPD analysis.

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Acknowledgements

This research received financial support of the European Community’s Seventh Framework Programme, Grant agreement 603629-ENV-2013-6.2.1. (Globaqua). This study represents a part of activities within the Project no. 170345, funded by the Ministry of Education, Science and Technological Development of the Republic of Serbia. We would like to express our appreciation to Ivan Nikolić for his suggestions and input regarding the RAPD method. The authors are especially grateful to Luka Gačić who provided English language improvement.

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Correspondence to Karolina Sunjog.

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Kolarević, S., Sunjog, K., Kračun-Kolarević, M. et al. The Genetic Variability (RAPD) and Genotoxicity In Vivo (Alkaline and Fpg-Modified Comet Assay) in Chub (Squalius cephalus): The Sava River Case Study. Int J Environ Res 12, 703–712 (2018). https://doi.org/10.1007/s41742-018-0127-6

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Keywords

  • DNA damage
  • RAPD fingerprints
  • In situ
  • Fish
  • River pollution