Tributyltin Affects Rat Vascular Contractility Through L-Type Calcium Channels
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The humans being exposed to tributyltin (TBT), through the ingestion of contaminated diet, particularly seafood, and through the ingestion of indoor dust. TBT is one of the most studied organotins and some reports demonstrated that this compound interferes with the physiology of the cardiovascular system; however, the exact mechanisms involved are still under discussion. Hence, this study aims to evaluate the effects of TBT on the vascular function. The evaluation of TBT effects on the contractility of rat aorta was performed using the organ bath technique using two different contractile agents: noradrenaline (NA) and potassium chloride (KCl). The whole-cell configuration of the patch clamp technique was performed to evaluate the TBT effects on the calcium currents of A7r5 cell line. The results demonstrate that TBT interferes with the vascular system as it elicits relaxation of the rat aorta contracted by NA or by KCl and inhibits L-type calcium currents in smooth muscle cells.
KeywordsTBT Cardiotoxicity Rat aorta A7r5 Patch clamp Organ bath
This investigation was supported by FEDER funds through the POCI—COMPETE 2020—Operational Programme Competitiveness and Internationalization in Axis I—Strengthening research, technological development and innovation (Project POCI-01-0145-FEDER-007491) and National Funds by FCT—Foundation for Science and Technology (Project UID/Multi/00709/2013). This work is also supported by the grant: SFRH/BD/131665/2017.
Compliance with Ethical Standards
Conflict of interest
The authors declare that they have no conflicts of interest.
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