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N-Acety-L-Cysteine-Stabilized Pt Nanozyme for Colorimetric Assay of Heparin

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Abstract

N-Acety-L-cysteine (NAC), enriched with multiple electron-rich groups involving –SH, –C=O, and –COOH, exhibits strong affinities to transition metal ions. Herein, NAC was adopted as the stabilizing agent to synthesize a series of peroxidase-like Pt nanoclusters (NCs) with different physicochemical properties. Both the cluster size and the charge state of NAC–Pt NCs are highly dependent upon the molar ratio of [K2PtCl4]/[NAC], contributing to different peroxidase mimicking activities. The most active Pt nanozyme (~ 1.7 nm), composed of 60% metallic Pt0 and 40% positively charged Pt2+ respectively, exhibits the Km of 0.132 mM for 3,3′,5,5′-tetramethylbenzidine and 35 mM for H2O2. Based on the heparin-activated enzymatic activity of NAC–Pt at pH 6.0, an ultrasensitive test was established for quantitative determination of heparin, giving the linear response of 0.5–20 μg/mL as well as the limit of detection of 2 × 10−3 μg/mL. This proposed method is also applicable in biological fluid for practical applications. Such a colorimetric method possesses several advantages involving high sensitivity, short response, eco-friendly synthesis, low consumption of material and energy, free of expensive instrument, as well as simple operation.

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Acknowledgements

This study was funded by National Natural Science Foundation of China (21878225, 21776215).

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Authors and Affiliations

  1. School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300350, People’s Republic of China

    Xinxue Li, Qingwei Huang, Wei Li, Jinli Zhang & Yan Fu

Authors
  1. Xinxue Li
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  2. Qingwei Huang
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  3. Wei Li
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  4. Jinli Zhang
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  5. Yan Fu
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Corresponding author

Correspondence to Yan Fu.

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Li, X., Huang, Q., Li, W. et al. N-Acety-L-Cysteine-Stabilized Pt Nanozyme for Colorimetric Assay of Heparin. J. Anal. Test. 3, 277–285 (2019). https://doi.org/10.1007/s41664-019-00108-w

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  • DOI: https://doi.org/10.1007/s41664-019-00108-w

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