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Pressurized Liquid Extraction of Pigments from Chlamydomonas sp. and Chemical Characterization by HPLC–MS/MS

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Abstract

Pressurized liquid extraction using green solvents (ethanol and water) was applied to extract photosynthetic pigments from the microalgae Chlamydomonas sp. An experimental design is employed to determine the effect of the extraction conditions on the extraction yield and the antioxidant capacity of the different extracts determined using 1,1-diphenyl-2-picryl-hydrazyl and TEAC tests. All the extracts show significant antioxidant activity in all assays. Besides, in this work, the carotenoid and chlorophyll composition of the Chlamydomonas extracts was analyzed by HPLC–DAD–APCI–MS/MS for the first time, being the main compounds lutein and pheophythin a´.

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Acknowledgements

This work was supported by EU MIRACLES project (Seventh Framework Programme KBBE- Grant Agreement No. 613588, Multi-product Integrated bioRefinery of Algae: from Carbon dioxide and Light Energy to high-value Specialties).

Author information

Correspondence to Jose A. Mendiola.

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Cite this article

Montero, L., Sedghi, M., García, Y. et al. Pressurized Liquid Extraction of Pigments from Chlamydomonas sp. and Chemical Characterization by HPLC–MS/MS. J. Anal. Test. 2, 149–157 (2018). https://doi.org/10.1007/s41664-018-0062-8

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Keywords

  • Antioxidant capacity
  • Chlamydomonas sp.
  • HPLC–DAD–APCI–MS/MS
  • Pigments
  • Pressurized liquid extraction
  • Carotenoids