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Efficacy of ultrasonic and Er:YAG laser activated EDTA irrigation in removing bacteria from ex vivo root canal system

Lasers in Dental Science volume 2, pages 265–269 (2018)Cite this article

Abstract

Purpose

Due to the limited effectiveness of irrigation to reduce the bacterial load inside root canals, the efficacy of activated irrigation techniques was evaluated.

Methods

Sixty endodontically prepared single-rooted human teeth were sterilized, infected with Enterococcus faecalis, and divided into six groups: (A) endodontic treatment positive control, (B) irrigation with EDTA 17%, (C) Er:YAG laser activated irrigation with tip 1 mm from working length, (D) Er:YAG laser to the coronal third, (E) ultrasonic irrigation applied 1 mm from working length, (F) US irrigation to the coronal third.

Results

The positive control group had significantly more bacteria than all other groups. EDTA irrigation had low efficiency. US irrigation 1 mm from the working length was significantly more effective than controls. Laser treatment to the coronal third was the most efficient.

Conclusions

Laser activated irrigation caused the greatest bacterial reductions.

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Authors and Affiliations

  1. Department of Endodontics, Faculty of Dental Medicine, School of Dental Medicine, The Hebrew University–Hadassah, P.O.B. 12272, 91120, Jerusalem, Israel

    Sharonit Sahar-Helft, Idan Redenski & Adam Stabholtz

  2. Biophotonics Laboratory, Institute of Biomedical Engineering Çengelköy, Boğaziçi University, Kandilli Kampüs, Istanbul, Turkey

    Ayşe Sena Kabaş Sarp & Murat Gulsoy

  3. Biofilm Research Laboratory, Institute of Dental Sciences, Faculty of Dental Medicine, School of Dental Medicine, The Hebrew University–Hadassah, Jerusalem, Israel

    Idan Redenski & Doron Steinberg

  4. Department of Endodontics, Sheba Medical Center, Israel Defense Force Medical Corps, Ramat Gan, Israel

    David Keinan

Authors
  1. Sharonit Sahar-Helft
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  2. Ayşe Sena Kabaş Sarp
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  3. Murat Gulsoy
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  4. Idan Redenski
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  5. Adam Stabholtz
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  6. David Keinan
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  7. Doron Steinberg
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Corresponding author

Correspondence to Sharonit Sahar-Helft.

Ethics declarations

The study was approved by the Hadassah Hospital Ethics Committee, approval no. 0118-14-HMO.

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

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Informed consent was obtained from all individual participants included in the study. [Optional].

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Cite this article

Sahar-Helft, S., Sarp, A.S.K., Gulsoy, M. et al. Efficacy of ultrasonic and Er:YAG laser activated EDTA irrigation in removing bacteria from ex vivo root canal system. Laser Dent Sci 2, 265–269 (2018). https://doi.org/10.1007/s41547-018-0046-z

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  • DOI: https://doi.org/10.1007/s41547-018-0046-z

Keywords

  • Biofilm
  • Ultrasonic
  • Laser
  • Root canal
  • Irrigation