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Effects of different Er,Cr:YSGG laser parameters on dentin shear bond strength and interface morphology: an in vitro study

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The aim of this in vitro study is to evaluate the effect of Er,Cr:YSGG laser parameters, specifically power (Watt) and frequency (Hertz), throughout both, shear bond strength (SBS) and dentin-composite interface morphology, when using laser etch as an alternative to acid etching with a “two-step” total etch adhesive.


Thirty-six human sound molars were mounted on acrylic blocks, randomly divided into six groups, six teeth into each group, where five teeth were selected for the SBS test, and one sectioned for the scanning electron microscope (SEM) analysis. Teeth were sliced occlusally to expose a flattened dentin surface, then grouped according to various treatments: control group, only treated with phosphoric acid etchant; and groups 1–5, only treated with Er,Cr,:YSGG laser irradiating at 3 W/25 Hz, 3 W/50 Hz, 4 W/25 Hz, 4 W/50 Hz, and 5 W/75 Hz respectively. The adhesive was applied followed by composite build up. SBS test was carried out using a universal testing machine. The resin-dentin interface was analyzed utilizing a SEM.


The control group showed the highest SBS values (11.38 ± 2.03 Mpa). Group 5 (5 W/75 Hz) was the second highest (8.46 ± 1.82 Mpa), yet the highest among the entire laser-irradiated groups, showing a marginal/borderline significance with the control group (P = 0.044). Group 3 (4 W/25 Hz) came second through the laser samples (7.41 ± 0.97 Mpa). SEM analysis manifested premier retentive composite dentin interface among the control group and groups 5 and 3.


When applying Er,Cr,:YSGG laser solely for dentin etching, adjusting the settings to a power of 5 W and frequency at 75 Hz will lead to optimum results regarding both SBS and interface morphology.

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The author would like to thank the following:

  1. (1)

    Professor Dr. med. dent. Norbert Gutknecht. Clinic for Conservative Dentistry, Periodontology and Preventive Dentistry, University Hospital Aachen, RWTH University. Scientific Director of Lasers in Dentistry Programs.

  2. (2)

    Mohamad Medhat Abdelsalam and Soha Negm El-din (financial and morale support).

  3. (3)

    Professor Dr. Rami Maher Ghali/Faculty of Dentistry-Ain Shams University’s Dental Laser Center.

  4. (4)

    Faculty of Dentistry-Misr International University’s Dental Laser Center.

  5. (5)

    Associate professor Dr. Emad abo El-azm. Operative Dentistry Department. Faculty of Dentistry-Suez Canal University (specimens’ preparations and measurements).

  6. (6)

    Dr. Khaled Keraa (statistical analysis).

  7. (7)

    Dr. Khaled El-Hadad, lecturer at Oral Biology Department. Faculty of Dentistry. Ain Shams University (SEM micrographs interpretation).

  8. (8)

    Mai Hussein (dental photography).

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Correspondence to Sherif Abdelsalam Mohamad.

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The author declares that there is no conflict of interest.

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This study does not include any human participants or animals. It was conducted on anonymous extracted teeth.

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Abdelsalam Mohamad, S. Effects of different Er,Cr:YSGG laser parameters on dentin shear bond strength and interface morphology: an in vitro study. Laser Dent Sci 2, 147–156 (2018).

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