To establish and validate a method for cell irradiation in 96-well and 6-well plates using a linear accelerator, three irradiation methods (G0B0F40, G0B1.5F40, and G180B1.5F40) were designed to irradiate cell culture plasticware simulated with RW3 slab phantom and polystyrene. The difference between the actual physical measured dose and the preset dose was compared among the three methods under the preparatory conditions of 2, 4, 6, 8, and 10 Gy. MDA-MB-231 cells were analyzed by using a cell proliferation assay and a clonogenic assay to verify the difference between the three cell irradiation methods on cell radiosensitivity. For each preset dose, the difference between the actual measured dose and the preset dose was the lowest for Method G0B1.5F40, the second lowest for Method G180B1.5F40, and the maximum for Method G0B0F40. The ranges of the differences were − 0.28 to 0.02%, − 2.17 to − 1.80%, and − 4.92 to − 4.55%, and 0.31 to − 0.12%, − 3.42 to − 2.86%, and − 7.31 to − 6.92%, respectively, for 96-well and 6-well plates. The cell culture experiments proved that Method G0B1.5F40 was an accurate, effective, simple, and practical irradiation method. The most accurate and effective cell irradiation method should always be used, as it will reduce dose differences and instability factors and provide improved accuracy and comparability for laboratories researching cellular radiosensitivity.
Linear accelerator Radiotherapy Cell irradiation methods Cell culture plate Dose
This is a preview of subscription content, log in to check access.
L. Van den Bosch, G. Defraene, S. Peeters et al., Is there a different dose-effect relation between the primary tumor and involved lymph nodes in locally advanced non-small-cell lung cancer? A hypothesis-generating study. Acta Oncol. 56, 541–547 (2017). https://doi.org/10.1080/0284186X.2017CrossRefGoogle Scholar
S.G. Mikalsen, N.E. Jeppesen, J.A. Sandvik et al., Separation of two sub-groups with different DNA content after treatment of T-47D breast cancer cells with low dose-rate irradiation and intermittent hypoxia. Acta Radiol. 49, 1–9 (2017). https://doi.org/10.1177/0284185117699999Google Scholar
X.R. Yang. Study of Biological Effects and Proteomics on Breast Cancer Cells MDA-MB-231 Induced by X-ray Irradiation. Ph.D. Thesis, Lanzhou University (2016) (in Chinese)Google Scholar
X.X. Chen. Clinical Analysis of Radiotherapy Effect and In Vitro Radiosensitivity Study for Triple Negative Breast Cancers. Ph.D. thesis, Fudan University Shanghai Cancer Center (2013) (in Chinese)Google Scholar
S. Fani, B. Kamalidehghan, K.M. Lo et al., Anticancer activity of a monobenzyltin complex C1 against MDA-MB-231 cells through induction of Apoptosis and inhibition of breast cancer stem cells. Sci. Rep. 6, 38992 (2016). https://doi.org/10.1038/srep38992CrossRefGoogle Scholar