Biological effects of human lung cells MRC-5 in CBCT positioning for image-guided radiotherapy

  • Chu-Feng Jin
  • Hui Liu
  • Wen-Yi Li
  • Rui-Fen Cao


Image-guided radiotherapy (IGRT) provides precise positioning for the tumor target, but it may bring extra irradiation dose in the target positioning with a cone beam CT (CBCT) which has been increasingly used in IGRT. In this work, we focused on biological effects of the low-dose irradiation in IGRT, which have not been considered so far. Primary human fibroblasts cells from the lung and MRC-5 were irradiated by a CBCT. DNA double-strand breaks (γ-H2AX foci) and micronucleus frequency of the irradiated samples were analyzed. Compared to the control, the γ-H2AX foci yields of the samples irradiated to 16 mGy increased significantly, and the micronuclei rate of the samples irradiated for 3 days increased notably. The dose by imaging guidance device can be genotoxic to normal tissue cells, suggesting a potential risk of a secondary cancer. The effects, if confirmed by clinical studies, should be considered prudentially in designing IGRT treatment plans for the radiosensitive population, especially for children.


Image-guided radiotherapy Cone beam CT Imaging irradiation Biological effects Secondary cancer 



The members of FDS Team have offered a large amount of help to this work. We thank Professor Lijun WU of Hefei Institutes of Physical Science, Chinese Academy of Sciences, for providing the MRC-5 cell line.


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Copyright information

© Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Chinese Nuclear Society, Science Press China and Springer Science+Business Media Singapore 2017

Authors and Affiliations

  • Chu-Feng Jin
    • 1
    • 2
  • Hui Liu
    • 2
  • Wen-Yi Li
    • 2
  • Rui-Fen Cao
    • 2
  1. 1.School of Nuclear Science and TechnologyUniversity of Science and Technology of ChinaHefeiChina
  2. 2.Key Laboratory of Neutronics and Radiation Safety, Institute of Nuclear Energy Safety TechnologyChinese Academy of SciencesHefeiChina

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