Biological effects of human lung cells MRC-5 in CBCT positioning for image-guided radiotherapy
Image-guided radiotherapy (IGRT) provides precise positioning for the tumor target, but it may bring extra irradiation dose in the target positioning with a cone beam CT (CBCT) which has been increasingly used in IGRT. In this work, we focused on biological effects of the low-dose irradiation in IGRT, which have not been considered so far. Primary human fibroblasts cells from the lung and MRC-5 were irradiated by a CBCT. DNA double-strand breaks (γ-H2AX foci) and micronucleus frequency of the irradiated samples were analyzed. Compared to the control, the γ-H2AX foci yields of the samples irradiated to 16 mGy increased significantly, and the micronuclei rate of the samples irradiated for 3 days increased notably. The dose by imaging guidance device can be genotoxic to normal tissue cells, suggesting a potential risk of a secondary cancer. The effects, if confirmed by clinical studies, should be considered prudentially in designing IGRT treatment plans for the radiosensitive population, especially for children.
KeywordsImage-guided radiotherapy Cone beam CT Imaging irradiation Biological effects Secondary cancer
The members of FDS Team have offered a large amount of help to this work. We thank Professor Lijun WU of Hefei Institutes of Physical Science, Chinese Academy of Sciences, for providing the MRC-5 cell line.
- 14.M. Fenech, A.A. Morley, Measurement of micronuclei in lymphocytes. Mutat. Res./Environ. Mutagen. Relat. Subj. 147(1–2), 29–36 (1985)Google Scholar