Abstract
In fall 2021, reddish and wilting of leaves and crown rot were observed on cauliflower plants with an incidence of up to 60% in a field in Eboli, South Italy. The causal agent was identified as Globisporangium perplexum based on morphological features of the colonies and by microscopy analyses. The identification was further confirmed by sequencing the internal transcribed spacer (ITS) region of rDNA. The pathogenicity tests reproduced symptoms under laboratory conditions that matched those observed in the field. To our knowledge, this is the first report of Globisporangium perplexum causing crown rot and wilting on cauliflower in Italy and worldwide.
References
Abdelzaher HMA (2010) Biological control of root rot of cauliflower (caused by Pythium ultimum var. ultimum) using selected antagonistic rhizospheric strains of Bacillus subtilis. N Z J Crop Hortic Sci 31:209–220. https://doi.org/10.1080/01140671.2003.9514255
Aegerter BJ, Davis RM (1998) First report of Pythium polymastum on broccoli and cauliflower in California. Plant Dis 82:1282–1282. https://doi.org/10.1094/PDIS.1998.82.11.1282A
Bella P, Moretti C, Licciardello G, Strano CP, Pulvirenti A, Alaimo S, Zaccardelli M, Branca F, Buonaurio R, Vicente JG, Catara V (2019) Multilocus sequence typing analysis of Italian Xanthomonas campestris pv. campestris strains suggests the evolution of local endemic populations of the pathogen and does not correlate with race distribution. Plant Pathol 68:278–287. https://doi.org/10.1111/ppa.12946
Candido V, Galgano F, Castronuovo D, Miccolis V, Favati F (2013) Quality traits of some cauliflower cultivars grown in the “valle dell’ Ofanto" area (Italy) as affected by post-harvest storage. Acta Hortic 1005:405–410. https://doi.org/10.17660/ACTAHORTIC.2013.1005.48
Capella-Gutiérrez S, Silla-Martínez JM, Gabaldón T (2009) trimAl: a tool for automated alignment trimming in large-scale phylogenetic analyses. Bioinformatics 25(15):1972–1973. https://doi.org/10.1093/bioinformatics/btp348
Chen JJ, Zong SB, Huang XX, Yu J, Yang HJ, Ye WW (2022) Molecular and morphological characterization of two new species of Globisporangium from Southern China, G. pengfuense and G. tenuihyphum. Diversity. https://doi.org/10.3390/D14070528
Dixon GR (2006) Vegetable brassicas and related crucifers. Veg Brassicas Relat Crucif. https://doi.org/10.1079/9780851993959.0000
El-Mohamedy RSR, El-Mougy N (2009) Occurrence of pythium rot of chinese cabbage in Egypt and its biocontrol measures. J Plant Prot Res 49:309–318. https://doi.org/10.2478/V10045-009-0050-0
Galgano F, Favati F, Caruso M, Pietrafesa A, Natella S (2007) The influence of processing and preservation on the retention of health-promoting compounds in broccoli. J Food Sci. https://doi.org/10.1111/J.1750-3841.2006.00258.X
Galland D, Paul B (2001) Pythium perplexum isolated from soil in France: morphology, molecular characterisation and biological control. Microbiol Res 156:185–189. https://doi.org/10.1078/0944-5013-00101
Gull C, Labuschagne N, Botha WJ (2014) Pythium species associated with wilt and root rot of hydroponically grown crops in South Africa. Afr Plant Prot 10:2
Hu J (2022) First report of Globisporangium heterothallicum causing seedling disease on guayule in Arizona. Plant Dis. https://doi.org/10.1094/PDIS-12-21-2644-PDN
ISTAT, 2021. Coltivazioni : Ortive [WWW Document]. Coltiv. ortive. URL http://dati.istat.it/index.aspx?queryid=33703 (Accessed 10 June 2022)
Kumar S, Stecher G, Li M, Knyaz C, Tamura K (2018) MEGA X: Molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 35(6):1547–1549. https://doi.org/10.1093/molbev/msy096
Kouyeas & Theohari, 1977. Pythium perplexum. Annls Inst Phytopath 11: 282
Larsen ML, Schlatter DD, Nimpoeno MJ, Hines-Snider MC, Samac DD (2023) Rhizosphere and root community analysis of oomycetes associated with poor alfalfa (Medicago sativa) seedling establishment. Phytobiomes J. https://doi.org/10.1094/PBIOMES-02-23-0005-R
Lazreg F, Belabid L, Sanchez J, Gallego E, Garrido-Cardenas JA, Elhaitoum A (2013) First report of Globisporangium ultimum causing pythium damping-off on aleppo pine in Algeria, Africa, and the Mediterranean Region. Plant Dis 97:1111. https://doi.org/10.1094/PDIS-07-12-0625-PDN
Le DP, Smith MK, Aitken EAB (2016) An assessment of Pythium spp. associated with soft rot disease of ginger (Zingiber officinale) in Queensland, Australia. Australas Plant Pathol 45:377–387. https://doi.org/10.1007/S13313-016-0424-5/FIGURES/4
Lévesque CA, De Cock AWAM (2004) Molecular phylogeny and taxonomy of the genus Pythium. Mycol Res 108:1363–1383. https://doi.org/10.1017/S0953756204001431
Li YP, You MP, Barbetti MJ (2014) Species of Pythium associated with seedling root and hypocotyl disease on common bean (Phaseolus vulgaris) in Western Australia. Plant Dis. https://doi.org/10.1094/PDIS-12-13-1231-RE
Mahmud KA (1950) Damping-off of cabbage, cauliflower and knolkohl caused by Pythium aphanidermatum. Fitz Curr Sci 19
Manganiello G, Pane C (2021) First report of Globisporangium ultimum causing damping-off of wild rocket seedlings. J Plant Pathol 103:721. https://doi.org/10.1007/S42161-021-00798-1/METRICS
McLeod A, Botha WJ, Meitz JC, Spies CFJ, Tewoldemedhin YT, Mostert L (2009) Morphological and phylogenetic analyses of Pythium species in South Africa. Mycol Res 113:933–951. https://doi.org/10.1016/J.MYCRES.2009.04.009
Moralejo E, Clemente A, Descals E, Belbahri L, Calmin G, Lefort F, Spies CFJ, McLeod A (2008) Pythium recalcitrans sp. nov. revealed by multigene phylogenetic analysis. Mycologia 100:310–319. https://doi.org/10.3852/MYCOLOGIA.100.2.310
Ramírez M, Pérez LM, Montealegre JR (2018) Susceptibility of different grapevine (Vitis vinifera L.) cultivars to Diplodia seriata and Diplodia mutila. Cienc e Investig Agrar 45:93–98. https://doi.org/10.7764/RCIA.V45I1.1818
Riit T, Tedersoo L, Drenkhan R, Runno-Paurson E, Kokko H, Anslan S (2016) Oomycete-specific ITS primers for identification and metabarcoding. MycoKeys 14:17–30. https://doi.org/10.3897/MYCOKEYS.14.9244
Schmidt CS, Leclerque A, Pfeiffer T, Goessling JW, Orlik M, Jamshidi B, Saar K, Sellmann J, Siepe I, Koch E (2020) Pathogenicity of Pythium species to maize. Eur J Plant Pathol 158:335–347. https://doi.org/10.1007/S10658-020-02076-9/TABLES/4
Singh SL, Pavgi MS (1977) Attraction of Pythium butleri zoospores to cauliflower roots. Phytopathol Mediterr 16:126–128
Singh SL, Pavgi MS (1978) Pythium root rot of crucifers. Mycopathologia 63:155–159. https://doi.org/10.1007/BF00490930/METRICS
Song WW, Guo M, Zhou YN, Liang C (2019) First report of cauliflower root rot caused by Pythium coloratum in China. Plant Dis 103:2697–2697. https://doi.org/10.1094/PDIS-02-19-0243-PDN
Stevens (1974) Mycology guidebook
Tompkins CM, Tucker CM, Gardner MW, (1936) Phytophthora root rot of Cauliflower. J Agric Res 53
Uzuhashi S, Tojo M, Kakishima M (2010a) Phylogeny of the genus Pythium and description of new genera. Mycoscience 51:337–365. https://doi.org/10.1007/S10267-010-0046-7
Uzuhashi S, Tojo M, Kakishima M (2010b) First report of Globisporangium ultimum causing constriction on hypocotyl of Beta vulgaris in North Dakota, USA. Austin J Plant Biol 51:337–365. https://doi.org/10.1007/S10267-010-0046-7
van der Plaats-Niterink AJ (1981) Monograph of the genus. Pythium 21:242
Volden J, Bengtsson GB, Wicklund T (2009) Glucosinolates, l-ascorbic acid, total phenols, anthocyanins, antioxidant capacities and colour in cauliflower (Brassica oleracea L. ssp. botrytis); effects of long-term freezer storage. Food Chem 112:967–976. https://doi.org/10.1016/J.FOODCHEM.2008.07.018
Zitnick-Anderson KK, Norland JE, del Río Mendoza LE, Fortuna AM, Nelson BD (2017) Probability models based on soil properties for predicting presence-absence of Pythium in soybean roots. Microb Ecol 74:550–560. https://doi.org/10.1007/S00248-017-0958-2
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The authors thank Mr. Antonio Altieri for providing plant samples and for field reporting.
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Dell’Olmo, E., Battaglia, V. & Sigillo, L. First report of Globisporangium perplexum (syn: Pythium perplexum) causing wilting and crown rot on cauliflower (Brassica oleracea var. botrytis). J Plant Dis Prot 131, 243–250 (2024). https://doi.org/10.1007/s41348-023-00811-y
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DOI: https://doi.org/10.1007/s41348-023-00811-y