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Analysis of Bacillus mojavensis ZA1 volatile anti-pathogen substances against Colletotrichum coccodes

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Abstract

Volatile organic compounds (VOCs) produced by endophytic strains play important roles in enhancing plant resistance to pathogens; however, the VOCs produced Bacillus mojavensis ZA1 on the inhibiting ability of Colletotrichum coccodes remain unknown. Hence, the effects of VOCs in controlling potato anthracnose (C. coccodes) were investigated. Alcohols, ketones, phenols, and esters were antifungal volatile compounds in the volatile organic compounds (VOCs) produced by B. mojavensis ZA1 after fermentation. The number of VOCs was 29 and 49 at extraction temperatures of 28 °C and 40 °C, respectively. The antifungal activity of 13 of these compounds was tested, and the results showed that 2-ethyl-1-hexanol completely inhibited mycelial growth, sporulation, and conidial germination of C. coccodes. The antifungal activity of VOCs; 2-methyl-1-butanol, 4,4′-(1-methylethylidene) bis-phenol, 2-nitro-ethanol, 2,3-butanediol and 3-methyl-1-butanol was also impressive; not only did they inhibit mycelial growth and spore germination by more than 43%, but they also reduced the pathogen’s conidia production by 43-fold. After studying the structure of organic volatiles with high fungistatic activity, it was predicted that the hydroxyl group could be an effective functional group for B. mojavensis ZA1 to achieve fungal suppression. The antifungal activity of metabolites of alcohols and ketones with 4–8 carbon atoms was superior against pathogenic fungus. As a result, carbon-chain length has been proposed as one of the parameters regulating the fungistatic efficacy of secondary metabolites. By utilizing metabolites produced by B. mojavensis ZA1, this research lays the groundwork for using B. mojavensis ZA1 in biological control.

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Acknowledgements

This study was supported by the project of the National Natural Science Foundation of China (No: 31660148) and by the Science and Technology Innovation Fund of Gansu Agricultural University (No. GAU-XKJS-2018-148) from Gansu Agricultural University to Prof. Chengde Yang.

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Authors and Affiliations

  1. College of Plant Protection, Gansu Agricultural University, Lanzhou, 730070, China

    Lijuan Wei, Lijuan Fan, Chengde Yang, Lingxiao Cui, Mengjun Jin & Richard Osei

  2. Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, Lanzhou, 730070, China

    Lijuan Wei, Lijuan Fan, Chengde Yang, Lingxiao Cui, Mengjun Jin & Richard Osei

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  1. Lijuan Wei
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Correspondence to Chengde Yang.

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Wei, L., Fan, L., Yang, C. et al. Analysis of Bacillus mojavensis ZA1 volatile anti-pathogen substances against Colletotrichum coccodes. J Plant Dis Prot 130, 633–642 (2023). https://doi.org/10.1007/s41348-023-00717-9

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