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Evaluation of the entomopathogenic fungi Beauveria bassiana culture filtrate for Meloidogyne incognita on tomato

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Abstract

In the present study, the effects of different concentrations of culture filtrate (1×, 5×, 10×, 20× and 50×) of two isolates of Beauveria bassiana (BIM-001 and BY2) on Meloidogyne incognita were investigated under controlled conditions on tomato. The control effect (%) of culture filtrate concentrations compared to that of the positive controls, Nostalgist (0.25 ml/l) (B. bassiana strain Bb-1) and Velum (0.16 ml/l) (Fluopyram). It was determined that the control effect of all concentrations of both isolates was significantly higher than the negative control (sterile distilled water). At undiluted concentration of the filtrate (1×), the control effect of BIM-001 and BY2 isolates was found to be over 70% on galls, egg masses, and soil J2 density. In addition, the most dilute concentration (50×) of BIM-001 decreased root galls, egg masses, and soil J2 density of M. incognita by approximately 35%, while BY2 suppressed about 14.5%. In the study, the chemical nematicide Velum provided the highest control (98%) on the number of galls and egg masses formed by M. incognita on tomato roots and soil J2 density. At the same time, 1× and 5× concentrations of BIM-001 ranked second after Velum. BIM-001 was found to have higher nematicidal effect than BY2. The nematicidal effect of Nostalgist was lower than that of the 1× and 5× concentrations of BIM-001 and BY2 isolates. These results indicate that the entomopathogenic fungus B. bassiana is a good alternative for the biocontrol of M. incognita.

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References

  • Alkan N, Söğüt MA, Göze Özdemir FG, Eken C (2016) Beauveria bassiana ve Lecanicillium muscarium kültür filtratlarının Meloidogyne incognita'ya karşı etkinlikleri, Türkiye VI. Bitki Koruma Kongresi, Konya, s.95 (in Turkish)

  • Amobonye A, Bhagwat P, Pandey A, Singh S, Pillai S (2020) Biotechnological potential of Beauveria bassiana as a source of novel biocatalysts and metabolites. Crit Rev Biotechnol 40(7):1019–1034

    Article  CAS  PubMed  Google Scholar 

  • Arya R (2011) Effect of culture filtrates of Trichoderma viridae and Trichothecium roseum grown on different carbon and nitrogen sources on hatching and mortality of root-knot nematode (Meloidogyne incognita). Indian J Nematol 41(1):62–70

    CAS  Google Scholar 

  • Aslan A, Elekcioğlu IH (2022) Biochemical and molecular identification of root-knot nematodes in greenhouse vegetable areas of Eastern Mediterranean Region (Turkey). Turk J Entomol 46(1):115–127

    Article  Google Scholar 

  • Aydınlı G, Mennan S (2019) Reproduction of root-knot nematode isolates from the middle Black Sea Region of Turkey on tomato with Mi-1.2 resistance gene. Turk J Entomol 43(4):417–427

    Article  Google Scholar 

  • Bamisile BS, Dash CK, Akutse KS, Keppanan R, Wang L (2018) Fungal endophytes: beyond herbivore management. Front Microbiol 9:544

    Article  PubMed  PubMed Central  Google Scholar 

  • Binod P, Sukumaran RK, Shirke SV, Rajput JC, Pandey A (2007) Evaluation of fungal culture filtrate containing chitinase as a biocontrol agent against Helicoverpa armigera. J Appl Microbiol 103:1845–1852

    Article  CAS  PubMed  Google Scholar 

  • Cetintas R, Abed Salih B (2016) Effect of Beauveria bassiana and Isaria tenuipes on the root-knot nematode, Meloidogyne incognita infesting tomato and eggplant. Egypt J Biol Pest Control 26(4):707–712

    Google Scholar 

  • da Silva JCP, Campos VP, Barros AF, Pedroso LA, de Freitas SM, de Souza JT, de Medeiros FHV (2019) Performance of volatiles emitted from different plant species against juveniles and eggs of Meloidogyne incognita. Crop Prot 116:196–203

    Article  Google Scholar 

  • Devi G, Bora LC (2018) Effect of some biocontrol agents against root-knot nematode (Meloidogyne incognita race2). Int J Environ Agric Biotechnol 3(5):265–260

    Google Scholar 

  • Devran Z, Söğüt MA (2010) Occurrence of virulent root-knot nematode populations on tomatoes bearing the Mi gene in protected vegetable-growing areas of Turkey. Phytoparasitica 38(3):245–251

    Article  Google Scholar 

  • Evlice E, Bayram Ş (2016) Identification of root-knot nematode species (Meloidogyne spp.) (Nemata: Meloidogynidae) in the potato fields of Central Anatolia (Turkey) using molecular and morphological methods. Turk Bull Entomol 6:339–347

    Google Scholar 

  • Evlice E, Toktay H, Yatkın G, Erdoğuş FD, İmren M (2022) Population fluctuations of root-knot nematodes Meloidogyne chitwoodi and M. hapla under field conditions. Phytoparasitica 50(1):233–242

    Article  CAS  Google Scholar 

  • Goettel S, Eilenberg J, Glare T (2005) Entomopathogenic fungi and their role in regulation of insect populations. In: Gilbert LB, Latrou K (eds) Comprehensive molecular insect science. Elsevier, Oxford, pp 361–406

    Chapter  Google Scholar 

  • Goverse A, Smant G (2014) The activation and suppression of plant innate immunity by parasitic nematodes. Annu Rev Phytopathol 52:243–265

    Article  CAS  PubMed  Google Scholar 

  • Göze Özdemir FG, Arıcı ŞE (2021) Effect of culture filtrate concentration of Rhizoctonia solani Kühn against Meloidogyne incognita and Meloidogyne hapla in vitro. Int J Agric for Life Sci 5(1):74–79

    Google Scholar 

  • Göze Özdemir FG, Uzun Yiğit A, Demirözer O (2022a) Determınatıon of Nematısıdal effect of Beauveria bassiana isolates on Meloidogyne incognita in vıtro. V. In: International health sciences and life congress 10–2 March 2022a Burdur, Turkey, pp 412–421

  • Göze Özdemir FG, Uzun Yiğit A, Demirözer O (2022b) Assessment of Nematicidal activity of Beauveria bassiana (Bals.-Criv.) Vuill on Pratylenchus thornei (Sher et Allen)(Tylenchida: Pratylenchidae). Int J Agric Environ Food Sci 6(1):65–71

    Article  Google Scholar 

  • Gürkan B, Çetintaş R, Gürkan T (2019) Determination of Root-Nematode species (Meloidogyne spp.) and some nematode population races in vegetable areas of Gaziantep and Osmaniye. KSU J Agric Nat 22(Suppl 1):113–124 (in Turkish with abstract in English)

    Google Scholar 

  • Hajihassani A, Marquez J, Woldemeskel M, Hamidi N (2022) Identification of four populations of Meloidogyne incognita in Georgia, United States, capable of parasitizing tomato-bearing Mi-1.2 gene. Plant Dis 106(1):137–143

    Article  CAS  PubMed  Google Scholar 

  • Haydock PPJ, Woods SR, Grove IG, Hare MC (2013) Chemical control of nematodes. In: Perry RN, Moens M (eds) Plant nematology, 2nd edn. CABI, Wallingford, pp 459–477

    Chapter  Google Scholar 

  • Hooper DJ, Hallmann J, Subbotin SA (2005) Methods for extraction and processing. In: Luc M, Sikora RA, Bridge J (eds) Plant parasitic nematodes in subtropical and tropical agriculture, 2nd edn. CAB International, Wallingford, pp 53–84

    Chapter  Google Scholar 

  • Hummadi EH, Dearden A, Generalovic T, Clunie B, Harrott A, Cetin Y, Butt T (2021) Volatile organic compounds of Metarhizium brunneum influence the efficacy of entomopathogenic nematodes in insect control. Biol Control 155:104527

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Hussey RS, Barker KR (1973) A comparison of methods of collecting inocula of Meloidogyne spp. including a new technique. Plant Dis Rep 57:1025–1028

    Google Scholar 

  • Ji X, Li J, Dong B, Zhang H, Zhang S, Qiao K (2019) Evaluation of fluopyram for southern root-knot nematode management in tomato production in China. Crop Prot 122:84–89

    Article  CAS  Google Scholar 

  • Karabörklü S, Aydınlı V, Dura O (2022) The potential of Beauveria bassiana and Metarhizium anisopliae in controlling the root-knot nematode Meloidogyne incognita in tomato and cucumber. J Asia-Pac Entomol 25(1):101846

    Article  Google Scholar 

  • Kaşkavalcı G (2007) Effects of soil solarization and organic amendment treatments for controlling Meloidogyne incognita in tomato cultivars in Western Anatolia. Turk J Agric for 31(3):159–167

    Google Scholar 

  • Kepenekci I, Saglam HD, Oksal E, Yanar D, Yanar Y (2017) Nematicidal activity of Beauveria bassiana (Bals.-Criv.) Vuill. against root-knot nematodes on tomato grown under natural conditions. Eagypt J Bıol Pest Control 27(1):117–120

    Google Scholar 

  • Khoja S, Eltayef KM, Baxter I, Myrta A, Bull JC, Butt T (2021) Volatiles of the entomopathogenic fungus, Metarhizium brunneum, attract and kill plant parasitic nematodes. Biol Control 152:104472

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kim JS, Roh JY, Choi JY, Wang Y, Shim HJ, Je YH (2010) Correlation of the aphicidal activity of Beauveria bassiana SFB-205 supernatant with enzymes. Fungal Biol 114:120–128

    Article  CAS  PubMed  Google Scholar 

  • Kim JJ, Jeong G, Han JH, Lee S (2013) Biological control of aphid using fungal culture and culture filtrates of Beauveria bassiana. Mycobiology 41(4):221–224

    Article  PubMed  PubMed Central  Google Scholar 

  • Li ZZ, Huang B, Chen MJ, Wang B, Fan MZ (2011) Studies on the genus Beauveria in molecular era. Mycosystema 30:823–835

    Google Scholar 

  • Li J, Zou C, Xu J, Ji X, Niu X, Yang J, Zhang KQ (2015) Molecular mechanisms of nematode–nematophagous microbe interactions: basis for biological control of plant-parasitic nematodes. Annu Rev Phytopathol 53:67–95

    Article  CAS  PubMed  Google Scholar 

  • Liu T, Wang L, Duan YX, Wang X (2008) Nematicidal activity of culture filtrate of Beauveria bassiana against Meloidogyne hapla. World J Microbiol Biotechnol 24(1):113–118

    Article  CAS  Google Scholar 

  • Lopez-Llorca LV, Maciá-Vicente JG, Jansson H-B (2008) Mode of action and interactions of nematophagous fungi. In: Ciancio A, Mukerji KG (eds) Integrated management and biocontrol of vegetable and grain crops nematodes. Springer, Berlin, pp 49–74

    Google Scholar 

  • Lu W, Zhang L, Guan M, Dong J (2016) Bioactivities of fermentation filtrate of 10 Beauveria bassiana strains on plant nematode and entomopathogenic nematode. J Henan Agric Sci 45(2):82–86

    Google Scholar 

  • Luo Z, Zhang T, Liu P, Bai Y, Chen Q, Zhang Y (2018) The Beauveria bassiana gas 3 β-glucanosyltransferase contributes to fungal adaptation to extreme alkaline conditions. Appl Environ Microbiol 84:e0108618

    Article  Google Scholar 

  • McKinnon AC, Glare TR, Ridgway HJ, Mendoza-Mendoza A, Holyoake A, Godsoe WK (2018) Detection of the entomopathogenic fungus Beauveria bassiana in the rhizosphere of wound-stressed Zea mays plants. Front Microbiol 9:1161

    Article  PubMed  PubMed Central  Google Scholar 

  • Misiha PK, Aly AZ, Mahrous ME, Tohamy MRA (2013) Effect of culture fılterates of three Trichoderma specıes, Fusarium solani and Rhizoctonia solani on egg hatchıng and juvenıle mortalıty of Meloidogyne incognita ın vıtro. Zagazig J Agric Res 40(3):1–9

    Google Scholar 

  • Mohanty SS, Prakash S (2009) Effects of culture media on larvicidal property of secondary metabolites of mosquito pathogenic fungus Chrysosporium lobatum (Moniliales: Moniliaceae). Acta Trop 109:50–54

    Article  CAS  PubMed  Google Scholar 

  • Mohanty SS, Raghavendra K, Mittal PK, Dash AP (2008) Efficacy of culture filtrates of Metarhizium anisopliae against larvae of Anopheles stephensi and Culex quinquefasciatus. J Ind Microbiol Biotechnol 35:1199–1202

    Article  CAS  PubMed  Google Scholar 

  • Moosavi MR, Zare R (2020) Fungi as biological control agents of plant-parasitic nematodes. In: Mérillon J-M, Ramawat KG (eds) Plant defence: biological control. Springer, Cham, pp 333–384

    Chapter  Google Scholar 

  • Naz I, Khan RA, Masood T, Baig A, Siddique I, Haq S (2021) Biological control of root knot nematode, Meloidogyne incognita, in vitro, greenhouse and field in cucumber. Biol Control 152:104429

    Article  CAS  Google Scholar 

  • Nishi O, Sushida H, Higashi Y, Iida Y (2020) Epiphytic and endophytic colonisation of tomato plants by the entomopathogenic fungus Beauveria bassiana strain GHA. Mycology 12(1):39–47

    Article  PubMed  PubMed Central  Google Scholar 

  • Ortiz-Urquiza A, Riveiro-Miranda L, Santiago-Álvarez C, Quesada-Moraga E (2010) Insect-toxic secreted proteins and virulence of the entomopathogenic fungus Beauveria bassiana. J Invertebr Pathol 105(3):270–278

    Article  CAS  PubMed  Google Scholar 

  • Ownley BH, Gwinn KD, Vega FE (2010) Endophytic fungal entomopathogens with activity against plant pathogens: ecology and evolution. Biocontrol 55:113–128

    Article  Google Scholar 

  • Pedrini N (2022) The entomopathogenic fungus Beauveria bassiana shows its toxic side within insects: expression of genes encoding secondary metabolites during pathogenesis. J Fungi 8(5):488

    Article  CAS  Google Scholar 

  • Quesada-Moraga E, Alain V (2004) Bassiacridin, a protein toxic for locusts secreted by the entomopathogenic fungus Beauveria bassiana. Mycol Res 108(4):441–452

    Article  CAS  PubMed  Google Scholar 

  • Rahoo AM, Mukhtar T, Abro SI, Bughio BA, Rahoo RK (2018) Comparing the productivity of five entomopathogenic nematodes in Galleria mellonella. Pak J Zool 50(2):679–684

    Article  Google Scholar 

  • Rehner SA, Minnis AM, Sung GH, Luangsa-ard JJ, Devotto L, Humber RA (2011) Phylogeny and systematics of the anamorphic, entomopathogenic genus Beauveria. Mycologia 103:1055–1073

    Article  PubMed  Google Scholar 

  • Seid A, Fininsa C, Mekete T, Decraemer W, Wesemael WM (2015) Tomato (Solanum lycopersicum) and root knot nematode (Meloidogyne spp.) a century old battle. Nematology 17:195–1000

    Article  Google Scholar 

  • Shan LT, Feng MG (2010) Evaluation of the biocontrol potential of various Metarhizium isolates against green peach aphid Myzus persicae (Homoptera: Aphididae). Pest Manag Sci 66:669–675

    Article  CAS  PubMed  Google Scholar 

  • Sikora RA, Fernandez E (2005) Nematode parasites of vegetables. In: Luc MR, Sikora A, Bridge J (eds) Plant parasitic nematodes in subtropical and tropical agriculture. CABI publishing, Egham, pp 319–392

    Chapter  Google Scholar 

  • Tapia-Vázquez I, Montoya-Martínez AC, los Santos-Villalobos D, Ek-Ramos MJ, Montesinos-Matías R, Martínez-Anaya C (2022) Root-knot nematodes (Meloidogyne spp.) a threat to agriculture in Mexico: biology, current control strategies, and perspectives. World J Microbiol Biotechnol 38(2):1–18

    Article  Google Scholar 

  • Uysal G, Söğüt MA, Elekçioğlu IH (2017) Identification and distribution of root-knot nematode species (Meloidogyne spp.) in vegetable growing areas of Lakes Region in Turkey. Turk J Entomol 41(1):105–122

    Google Scholar 

  • Wang C, Skrobek A, Butt TM (2004) Investigations on the destruxin production of the entomopathogenic fungus Metarhizium anisopliae. J Invertebr Pathol 85:168–174

    Article  CAS  PubMed  Google Scholar 

  • Wang Y, Fan Q, Wang D, Zou WQ, Tang DX, Hongthong P, Yu H (2022) Species diversity and virulence potential of the Beauveria bassiana complex and Beauveria scarabaeidicola complex. Front Microbiol 13:841604

    Article  PubMed  PubMed Central  Google Scholar 

  • Wenya L, Zhang L, Mejluan G, Dong JZ (2016) Bioactivities of fermentation filtrate of 10 Beauveria bassiana strains on plant nematode and entomopathogenic nematode. J Henan Agric Sci 45(2):82–86

    Google Scholar 

  • Xiang C, Liu Y, Liu SM, Huang YF, Kong LA, Peng H, Huang WK (2020) αβ-Dehydrocurvularin isolated from the fungus Aspergillus welwitschiae effectively inhibited the behaviour and development of the root-knot nematode Meloidogyne graminicola in rice roots. BMC Microbiol 20(1):1–10

    Article  Google Scholar 

  • Yağcı M (2022) Determination of the efficacy of two local Beauveria bassiana (Bals.-Criv.) Vuill, 1912 (Hypocreales: Cordycipitaceae) isolates (Bb-1 and Bv-1) against root-knot nematodes. J Glob Innov Agric Sci 10(1):37–41

    Article  Google Scholar 

  • Youssef M, El-Nagdi W, Lotfy DE (2020) Evaluation of the fungal activity of Beauveria bassiana, Metarhizium anisopliae and Paecilomyces lilacinus as biocontrol agents against root-knot nematode, Meloidogyne incognita on cowpea. Bull Natl Res Centre 44(1):1–11

    Article  Google Scholar 

  • Zhai Y, Shao Z, Cai M, Zheng L, Li G, Huang D, Zhang J (2018) Multiple modes of nematode control by volatiles of Pseudomonas putida 1A00316 from Antarctic soil against Meloidogyne incognita. Front Microbiol 9:253

    Article  PubMed  PubMed Central  Google Scholar 

  • Zhao D, Liu B, Wang Y, Zhu X, Duan Y, Chen L (2013) Screening for nematicidal activities of Beauveria bassiana and associated fungus using culture filtrate. Afr J Microbiol Res 7(11):974–978

    CAS  Google Scholar 

  • Zimmermann G (2007) Review on safety of the entomopathogenic fungi Beauveria bassiana and Beauveria brongniartii. Biocontrol Scie Technol 17(6):553–596

    Article  Google Scholar 

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Acknowledgements

Olympos Seedling Production Facility and Agricultural Engineer Tuğçe Okumuş Erol are thanked for providing tomato seedlings.

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  1. Department of Plant Protection, Faculty of Agriculture, Isparta University of Applied Sciences, 32200, Isparta, Turkey

    Fatma Gül Göze Özdemir, Asiye Uzun Yiğit & Ozan Demirözer

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  1. Fatma Gül Göze Özdemir
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  2. Asiye Uzun Yiğit
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FGGO and AUY participated in the nematicidal effect bioassays and collected of data. OD performed statistically analysis of these data. FGGO, AUY and OD wrote the manuscript, read and approved the final manuscript.

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Correspondence to Fatma Gül Göze Özdemir.

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Göze Özdemir, F.G., Uzun Yiğit, A. & Demirözer, O. Evaluation of the entomopathogenic fungi Beauveria bassiana culture filtrate for Meloidogyne incognita on tomato. J Plant Dis Prot 130, 271–278 (2023). https://doi.org/10.1007/s41348-022-00698-1

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