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First report of Bacillus velezensis JK-F2 for the biological control of crown gall in kiwifruit

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Abstract

A highly pathogenic bacterium, Agrobacterium Fabacearum, causes crown gall in kiwifruit plants leads to serious damage resulting in huge economic losses. In this study, we aimed to screen antagonistic bacterial biocontrol agents against crown gall disease of kiwifruit plants. A total of 225 bacterial strains were isolated from kiwifruit plant rhizosphere soil. Among these, strain JK-F2 exhibited good biocontrol effects in the form of antagonistic activity against A. Fabacearum in vitro. Based on morphological, physiological, and biochemical features and 16S rRNA and gyrA genes, the strain JK-F2 was identified as Bacillus velezensis. The zone of inhibition of strain JK-F2 was 22.84 ± 0.21 mm when the plate was covered with A. Fabacearum. In pot tests with artificial inoculation of A. Fabacearum to kiwifruit plants, the application of strain JK-F2 ahead of pathogen inoculation significantly reduced the incidence of crown gall by 26.67%, and the control efficiency was 71.96%. In field experiments, the inoculation of strain JK-F2 by root irrigation reduced the average growth rate of galls by 17.95–43.15%. The undiluted strain JK-F2 was more effective than the diluted strain in controlling crown gall by reducing the growth rate of galls, with a biocontrol effect of 68.07%. In this study, strain JK-F2, with good biocontrol application value, was isolated and identified. It is a promising candidate for the biocontrol of crown gall in kiwi plants.

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Data availability

The datasets generated and/or analysed during the study are available from the corresponding author upon reasonable request.

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Funding

This work was supported by the agricultural research projects of the Science and Technology Department of Guizhou Province (No. [2019]2403, [2021]YIBAN237), the China Agriculture Research System of MOF (296-Ministry of Agriculture Project), the Talent Introducing Scientific Research Fund of Guizhou University (NO. X2021029), and the Support Plan Projects of Guiyang City (No. (2017)26–1).

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Authors and Affiliations

  1. College of Agriculture, Research Center for Engineering Technology of Kiwifruit, Institute of Crop Protection, Guizhou University, Guiyang, 550025, China

    Ting Ting Chen, Fei Ran, Jin Qiao Shi, Fei Xu Mo, Xian Hui Yin, Zhi Bo Zhao, Zhu Zhu Zhang, Wen Zhi Li, Li Nan He & You Hua Long

  2. Teaching Experimental Field of Guizhou University, Guiyang, 550025, China

    You Hua Long

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  1. Ting Ting Chen
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  2. Fei Ran
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  3. Jin Qiao Shi
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Chen, T., Ran, F., Shi, J.Q. et al. First report of Bacillus velezensis JK-F2 for the biological control of crown gall in kiwifruit. J Plant Dis Prot 129, 1153–1162 (2022). https://doi.org/10.1007/s41348-022-00634-3

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