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Biological and molecular properties of Prune dwarf virus cherry isolates from Bulgaria

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Abstract

Biological and molecular properties of five Prune dwarf virus cherry isolates were studied. Double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) proved PDV infection in the respective source trees and absence of six other viruses infecting the cherry. Two of infected source trees were symptomless, and the other three trees showed deformed and elongated leaves with chlorotic lines and spots. The reaction of greenhouse-grown seedlings of 22 herbaceous test plant species was investigated. The woody indicators Prunus tomentosa and Prunus cerasifera were chip-budded. Cucumis sativus cv. Amelia, C. sativus cv. Levina, C. sativus cv. Tessa and Prunus tomentosa appeared as the most suitable test plants for bioassay of studied PDV isolates. The nucleotide sequences corresponding to the full-length coat protein (CP) of 657 bp were obtained. The identity among studied isolates in CP was 95.5–100% and 97.4–100% at nucleotide and amino acid levels, respectively. The movement protein (MP) sequences were 753 nucleotides long, translating 251 amino acids and shared 94.1–98.4% nucleotide and 96.8–99.2% amino acid identity. Constructed trees with the nucleotide sequences of both MP and CP genes of studied isolates and referent PDV cherry variants showed the formation of two phylogroups in the two genomic regions. All studied isolates separated into two clusters in group I. No correlation between the phylogenetic grouping and the biological properties, as well the geographical origin, was observed.

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All data that support the findings reported in this study are available from the corresponding author upon reasonable request.

References

  • Bachman EJ, Scott SW, Xin G et al (1994) The complete nucleotide sequences of Prune dwarf ilarvirus RNA3: implications for coat protein activation of genome replication in ilarviruses. Virology 201:127–131

    Article  CAS  Google Scholar 

  • Brunt AA, Crabtree K, Dallwitz MJ, Gibbs AJ, Watson L (1996) Viruses of Plants. CAB International, Wallingford

    Google Scholar 

  • Bujarski JJ, Gallitelli D, Garcia-Arenal F, Pallas V, Palukaitis P, Reddy MK, Wand A (2019) ICTV virus taxonomy profile: Bromoviridae. J Gen Vitol 100:2006–1207. https://doi.org/10.1099/jgv.0.001282

    Article  CAS  Google Scholar 

  • Clark MF, Adams AN (1977) Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. J Gen Virol 34:475–483

    Article  CAS  Google Scholar 

  • Cui H, Hong N, Wang G, Wang A (2013) Genomic segments RNA1 and RNA2 of Prunus necrotic ringspot virus codetermine viral pathogenicity to adapt to alternating natural Prunus hosts. MPMI 26(5):515–527. https://doi.org/10.1094/MPMI-12-12-0282-R

    Article  CAS  PubMed  Google Scholar 

  • Damsteegt VD, Al S, Mink GI et al (1998) The versatility of Prunus tomentosa as a bioindicator of viruses. Acta Hortic 472:143–146

    Article  Google Scholar 

  • Flegg KL, Clark MF (1979) The detection of Apple chlorotic leaf spot virus by a modified procedure of enzyme-linked immunosorbent assay. Ann Appl Biol 91(1):61–65

    Article  Google Scholar 

  • Fonseca F, Neto JD, Martins V et al (2005) Genomic variability of Prune dwarf virus as affected by agricultural practice. Arch Virol 150:1607–1619

    Article  CAS  Google Scholar 

  • Fulton RW (1970) Prune dwarf virus. C.M.I./A.A.B. Description of Plant Viruses:19 (Unavailable online)

  • Garcia JA, Pallas V (2015) Viral factors involved in plant pathogenesis. Curr Opin Virol 11:21–30

    Article  CAS  Google Scholar 

  • Halk EI, Fulton RW (1978) Stabilization and particle morphology of Prune dwarf virus. Virology 91:434–443

    Article  CAS  Google Scholar 

  • Higgins D, Thompson J, Gibson T et al (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680

    Article  Google Scholar 

  • Kalinowska E, Mroczkowska K, Paduch-Cichal E, Chrorska M (2014) Genetic variability among coat protein of Prune dwarf virus variants from different countries and different Prunus species. Eur J Plant Pathol 140:863–868

    Article  CAS  Google Scholar 

  • Kamenova I, Borisova A, Popov A (2019) Incidence and genetic diversity of Prune dwarf virus in sweet and sour cherry in Bulgaria. Biotech Biotech Eq 33(1):980–987. https://doi.org/10.1080/13102818.2019.1637278

    Article  CAS  Google Scholar 

  • Kamenova I, Borisova A, Popov A (2020) Occurrence of Ilarviruses in sweet and sour cherry in Bulgaria. Bulg J Agric Sci 26(3):590–597

    Google Scholar 

  • Koziel E, Otulak K, Garbaczewska G (2015) Phylogenetic analysis of PDV movement protein compared to Bromoviridae members as justification of possible intracellular movement. Acta Biol Crac Series Bot 57(2):106–114

    CAS  Google Scholar 

  • Koziel E, Bujarski JJ, Otulak K (2017) Molecular biology of Prune dwarf virus - A lesser known member of the Bromoviridae but a vital component in the dynamic virus-host cell interaction network. Int J Mol Sci 18:2733

    Article  Google Scholar 

  • Kunze L, Clark MF, Flegg CL (1984) Comparison of methods for characterizing and distinguishing isolates of Prune dwarf virus. Phytopath Z 110:251–260

    Article  Google Scholar 

  • Massart S, Brostaux Y, Barbarossa L et al (2008) Inter-laboratory evaluation of a duplex RT-PCR method using crude extracts for the simultaneous detection of Prune dwarf virus and Prunus necrotic ringspot virus. Eur J Plant Pathol 122:539–547

    Article  CAS  Google Scholar 

  • Mink GI (1993) Pollen and seed-transmitted viruses and viroids. Annu Rev Phytopathol 31:375–402

    Article  CAS  Google Scholar 

  • Nemeth M (1986) Virus, mycoplasma and rikettsia diseases of fruit trees. Academia Kiado, London

    Google Scholar 

  • Ȫztürk Y, Cevik B (2015) Genetic diversity in the coat protein genes of Prune dwarf virus isolates from sweet cherry growing in Turkey. Plant Pathol J 31(1):41–49

    Article  Google Scholar 

  • Paduch-Cichal E (2000) Characterization of PNRSV and PDV. Associate Professor Thesis, Warsaw University of Live Sciences, Warsaw, Poland

  • Paduch-Cichal E, Sala-Rejczak K, Mroczkiowska K et al (2011) Serological characterization of Prune dwarf virus isolates. J Pant Protect Res 51(4):389–392

    Google Scholar 

  • Pallas V, Aparicio F, Herranz MC et al (2013) Molecular biology of Ilarviruses. Adv Virus Res 87:139–181

    Article  CAS  Google Scholar 

  • Predajňa L, Sihelská N, Benediková D et al (2017) Molecular characterization of Prune dwarf virus cherry isolates from Slovakia events shows their substantial variability and reveals recombination. Eur J Plant Pathol 147:877–885

    Article  Google Scholar 

  • Šutic DD, Ford RE, Tosic MT (1999) Virus diseases of fruit trees, In: Handbook of plant virus diseases, CRC Press, Boca Raton, FL, USA

  • Tamura K, Stecher G, Peterson D et al (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729

    Article  CAS  Google Scholar 

  • Ulubaş-Serçe Ç, Ertunç F, Oeztuerk A (2009) Identification and genomic variability of Prune dwarf virus variants infecting stone fruit trees in turkey. J Phytopathol 157:298–305

    Article  Google Scholar 

  • Vaskova D, Pertzik K, Spak J (2000) Molecular variability of the capsid protein of the Prune dwarf virus. Eur J Plant Pathol 106:573–580

    Article  CAS  Google Scholar 

  • Waterworth HE, Fulton RW (1964) Variation among isolates of necrotic ringspot and prune dwarf viruses isolated from sour cherry. Phytopathology 54:1155–1160

    Google Scholar 

Download references

Funding

This research was supported by National Science Fund, Ministry of Education and Science, Bulgaria, grant number DH16/7 dated 11.12.2017.

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Authors and Affiliations

  1. Biotic Stress Group, Agrobioinstitute, Agricultural Academy, Sofia, Bulgaria

    Ivanka L. Kamenova

  2. Department of Agrotechnology, Plant Protection and Economics On Crops, Institute of Agriculture, Agricultural Academy, Kyustendil, Bulgaria

    Aneliya Z. Borisova

Authors
  1. Ivanka L. Kamenova
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  2. Aneliya Z. Borisova
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Contributions

IK performed molecular analysis and drafted the manuscript. AB performed biological assays.

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Correspondence to Ivanka L. Kamenova.

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Kamenova, I.L., Borisova, A.Z. Biological and molecular properties of Prune dwarf virus cherry isolates from Bulgaria. J Plant Dis Prot 129, 301–311 (2022). https://doi.org/10.1007/s41348-021-00551-x

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