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Application of biochar and vermicompost against the rice root-knot nematode (Meloidogyne graminicola): an eco-friendly approach in nematode management

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Abstract

The rice root-knot nematode (Meloidogyne graminicola) is one of the key pests of rice (Oryza sativa L.) in irrigated and upland rice ecosystems inflicting about 20–80% yield loss. While chemical nematicides are widely used to combat this pest, they have a negative impact on the environment as well as on animal and human health. The present study evaluated the efficacy of organic amendments like biochar and vermicompost in managing the rice root-knot nematode (RRKN) at 0.6, 1.2, 2.5, and 5% (w/v) concentrations. Our result showed that biochar and vermicompost exudate neither exhibit any direct killing ability nor altered the infectivity of RRKN second-stage juveniles (J2). Second-stage juvenile hatching was, however, reduced in two higher doses (2.5% and 5%) of vermicompost exudates. When final nematode population was normalized with root weights at 21 days’ post inoculation, a reduction in population build-up was observed with increased doses of vermicompost. A reverse trend was observed for biochar treatments. Overall, our study showed that application of 1.2% biochar and 5% vermicompost could be helpful in mitigating the RRKN stress in rice. Hence, application of biochar and vermicompost could be an effective alternative to toxic chemical nematicides and recommended as eco-friendly management options against M. graminicola in rice.

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References

  • Agyarko K, Asante JS (2005) Nematode dynamics in soil amended with neem leaves and poultry manure. Asi J Plant Sci 4:426–428

    Article  Google Scholar 

  • Aira M, Monroy F, Domínguez J, Mato S (2002) How earthworm density affects microbial biomas and activity in pig manure. Eur J Soil Bio 38:7–10

    Article  Google Scholar 

  • Akhtar M, Alam MM (1993) Utilization of waste materials in nematode control: a review. Bioresour Technol 45:1–7

    Article  CAS  Google Scholar 

  • Bandumula N (2018) Rice production in Asia: Key to global food security. In: Proceedings of the National Academy of Sciences, India Section B: Biol. Sci. 88: 1323–1328

  • Barbosa KAG, Garcia RA, Santos LC, Teixeira RA, Araújo FG, Rocha MR, Lima FSO (2010) Avaliação da adubação potássica sobre populações de Heterodera glycines em cultivares de soja resistente esuscetível. Nematol Bras 34:150–157

    Google Scholar 

  • Bybd D Jr, Kirkpatrick T, Barker K (1983) An improved technique for clearing and staining plant tissues for detection of nematodes. J Nematol 15:142–143

    CAS  PubMed  PubMed Central  Google Scholar 

  • Cabasan MTN, Kumar A, De Waele D (2012) Comparison of migration, penetration, development and reproduction of Meloidogyne graminicola on susceptible and resistant rice genotypes. Nematol 14:405–415

    Article  Google Scholar 

  • Chen S, Dickson D (2000) A technique for determining live second stage juveniles of Heterodera glycines. J Nematol 32:117

    CAS  PubMed  PubMed Central  Google Scholar 

  • De Waele D, Elsen A (2007) Challenges in tropical plant nematology. Annu Rev Phytopathol 45:457–485

    Article  PubMed  Google Scholar 

  • Ding Y, Liu Y, Liu S, Li Z, Tan X, Huang X, Zeng G, Zhou L, Zheng B (2016) Biochar to improve soil fertility. A review. Agron Sustain Dev 36(2):36

    Article  Google Scholar 

  • Edwards CA, Burrows I (1988) Potential of earthworm composts as plant growth media. In: Earthworms in waste and environmental management. Clive A. Edwards and Edward F. Neuhauser (eds.). SPB Acad Pub 211–219.

  • Edwards CA, Arancon NQ, Sherman RL (2010) Vermiculture Technology: Earthworms. Organic Waste and Environmental Management. CRC Press, Taylor and Francis Group, LLC, USA

    Book  Google Scholar 

  • FAO (2017) The future of food and agriculture: trends and challenges. Food and Agriculture Organization of the United Nations, Rome, p 180

    Google Scholar 

  • Gajalakshmi S, Abbasi S (2004) Neem leaves as a source of fertilizer-cum-pesticide vermicompost. Bioresour Technol 92:291–296

    Article  CAS  PubMed  Google Scholar 

  • George C, Kohler J, Rillig MC (2016) Biochars reduce infection rates of the root-lesion nematode Pratylenchus penetrans and associated biomass loss in carrot. Soil Biol Biochem 95:11–18

    Article  CAS  Google Scholar 

  • Graber E, Frenkel O, Jaiswal A, Elad Y (2014) How may biochar influence severity of diseases caused by soilborne pathogens? Carbon Manag 5:169–183

    Article  CAS  Google Scholar 

  • Huang WK, Ji HL, Gheysen G, Debode J, Kyndt T (2015) Biochar-amended potting medium reduces the susceptibility of rice to root-knot nematode infections. BMC Plant Biol 15:267

    Article  PubMed  PubMed Central  Google Scholar 

  • Hussey RS, Barker KR (1973) A comparison of methods of collecting inocula of Meloidogyne spp., including a new technique. Plant Dis Rep 57:1025–1028

    Google Scholar 

  • Husson O (2013) Redox potential (Eh) and pH as drivers of soil/plant/microorganism systems: a transdisciplinary overview pointing to integrative opportunities for agronomy. Plant Soil 362:389–417

    Article  CAS  Google Scholar 

  • Jain RK, Khan MR, Kumar V (2012) Rice root-knot nematode (Meloidogyne graminicola) infestation in rice. Archiv Phytopathol Plant Protec 45:635–645

    Article  Google Scholar 

  • Jaiswal P, Singh K (2010) A technique for studying the life cycle of Meloidogyne graminicola in rice roots. Pest Sci. and Manag. Int Rice Res Notes 35:1–3

    Google Scholar 

  • Jaiswal KA, Elad Y, Graber RE, Frenkel O (2014) Rhizoctonia solani supression and plant growth promotion in cucumber as affected by biochar pyrolysis temperature, feedstock and concentration. Soil Biol Biochem 69:110–118

    Article  CAS  Google Scholar 

  • Joseph S, Camps-Arbestain M, Lin Y, Munroe P, Chia C, Hook J, Van Zwieten L, Kimber S, Cowie A, Singh B (2010) An investigation into the reactions of biochar in soil. Soil Res 48:501–515

    Article  CAS  Google Scholar 

  • Joshi R, Singh J, Vig AP (2015) Vermicompost as an effective organic fertilizer and biocontrol agent: effect on growth, yield and quality of plants. Rev Environ Sci Biotech 14:137–159

    Article  CAS  Google Scholar 

  • Kimpinski J, Gallant C, Henry R, Macleod J, Sanderson J, Sturz A (2003) Effect of compost and manure soil amendments on nematodes and on yields of potato and barley: A 7-year study. J Nematol 35:289–293

    CAS  PubMed  PubMed Central  Google Scholar 

  • Kumar V, Khan MR, Walia R (2020) Crop Loss Estimations due to Plant-Parasitic Nematodes in Major Crops in India. Natl Acad Sci Lett 1–4.

  • Kumari C, Dutta TK, Banakar P, Rao U (2016) Comparing the defence-related gene expression changes upon root-knot nematode attack in susceptible versus resistant cultivars of rice. Sci Rep 6:22846

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Laird DA, Fleming P, Davis DD, Horton R, Wang B, Karlen DL (2010) Impact of biochar amendments on the quality of a typical Midwestern agricultural soil. Geoderma 158:443–449

    Article  CAS  Google Scholar 

  • Lehmann J, Joseph S (2009) Biochar for Environmental Management: Science and Technology. Earthscane, London

    Google Scholar 

  • Lehmann J, Rillig MC, Thies J, Masiello C, Hockaday W, Crowley D (2011) Biochar effects on soil biota – A review. Soil Biol Biochem 43:1812–1836

    Article  CAS  Google Scholar 

  • Linford MB, Yap F, Oliveria JM (1938) Reduction of soil population of root knot nematode during decomposition of organic matter. Soil Sci 45:127–142

    Article  Google Scholar 

  • Mantelin S, Bellafiore S, Kyndt T (2017) Meloidogyne graminicola: a major threat to rice agriculture. Mol Plant Pathol 18:3–15

    Article  PubMed  Google Scholar 

  • Matlack GR (2001) Factors determining the distribution of soil nematodes in a commercial forest landscape. Forest Ecol Manag 146:129–143

    Article  Google Scholar 

  • Mcsorley R (2011) Overview of organic amendments for management of plant-parasitic nematodes, with case studies from Florida. J Nematol 43:69–81

    PubMed  PubMed Central  Google Scholar 

  • Merny G, Luc M (1969) Les techniques d’échantillonnage des peuplements de nématodes dans le sol. Problèmes d’Qco1ogie:l’échantillonnage des peuplementf animaux des milieux terrestres. Masson & Cie, Paris, France, pp 237–272

    Google Scholar 

  • Mishra S, Wang KH, Sipes BS, Tian M (2017) Suppression of root-knot nematode by vermicompost tea prepared from different curing ages of vermicompost. Plant Dis 101:734–737

    Article  CAS  PubMed  Google Scholar 

  • Mishra S, Wang KH, Sipes B, Tian M (2019) Induction of host-plant resistance in cucumber by vermicompost tea against root-knot nematode. Nematropica 48:164–171

    Google Scholar 

  • Murugan S, Gu S (2015) Research and development activities in pyrolysis–Contributions from Indian scientific community–A review. Renew Sust Energ Rev 46:282–295

    Article  Google Scholar 

  • Nielson R (1965) Presence of plant growth substances in earthworms demonstrated by paper chromatography and the Went pea test. Nature 208:1113–1114

    Article  Google Scholar 

  • Nitao JK, Meyer SL, Chitwood DJ (1999) In-vitro assays of Meloidogyne incognita and Heterodera glycines for detection of nematode-antagonistic fungal compounds. J Nematol 31:172

    CAS  PubMed  PubMed Central  Google Scholar 

  • Oka Y (2010) Mechanisms of nematode suppression by organic soil amendments—a review. Appl Soil Ecol 44:101–115

    Article  Google Scholar 

  • Oyebanji O, Nweke O, Odebunmi O, Galadima N, Idris M, Nnodi U, Afolabi A, Ogbadu G (2009) Simple, effective and economical explant-surface sterilization protocol for cowpea, rice and sorghum seeds. Afr J Biotechnol 8:5395–5399

    CAS  Google Scholar 

  • Padgham JL (2004) Impact of the rice root-knot nematode (Meloidogyne graminicola) on lowland rainfed rice in northwestern Bangladesh. J Nematol 36(1):42–48

    CAS  PubMed  PubMed Central  Google Scholar 

  • Page AL, Miller RH, Keeney DR (eds) (1982) Methods of soil analysis; 2 Chemical and microbiological properties, Agronomy Monograph 92. Madison, WI, USA, ASA

    Google Scholar 

  • Renčo M (2013) Organic amendments of soil as useful tools of plant parasitic nematodes control. Helminthologia 50:3–14

    Article  Google Scholar 

  • Renčo M, Kováčik P (2012) Response of plant parasitic and free living soil nematodes to composted animal manure soil amendments. J Nematol 44:329–336

    PubMed  PubMed Central  Google Scholar 

  • Rostami M, Olia M, Arabi M (2014) Evaluation of the effects of earthworm Eisenia fetida-based products on the pathogenicity of root-knot nematode (Meloidogyne javanica) infecting cucumber. Int J Recycl Org Waste Agricult 3:58

    Article  Google Scholar 

  • Salalia R, Walia RK, Somvanshi VS, Kumar P, Kumar A (2017) Morphological, morphometric, and molecular characterization of intraspecific variations within Indian populations of Meloidogyne graminicola. J Nematol 49:254–267

    Article  PubMed  PubMed Central  Google Scholar 

  • Schindler A (1961) A simple substitute for a Baermann funnel. Plant Dis Report 45:747

    Google Scholar 

  • Sharf R, Hisamuddin A, Akhtar A (2014) Management of root-knot disease in Phaseolus vulgaris using potassium fertilizer and biocontrol agents. J Plant Pathol Microbiol 5:2

    Google Scholar 

  • Singh B, Hatton B, Singh B, Cowie A, Kathuria A (2010) Influence of biochars on nitrous oxide emission and nitrogen leaching from two contrasting soils. J Environ Qual 39:1224–1235

    Article  CAS  PubMed  Google Scholar 

  • Soriano I, Reversat G (2003) Management of Meloidogyne graminicola and yield of upland rice in South-Luzon, Philippines. Nematol 5:879–884

    Article  Google Scholar 

  • Sun Y, Yin J, Cao H, Li C, Kang L, Ge F (2011) Elevated CO2 influences nematode-induced defense responses of tomato genotypes differing in the JA pathway. PLoS ONE 6:e19751

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Sundquist B (2007) The Earth’s carrying capacity—Chapter 2: Parameters useful for perspective and calculation.

  • Tandingan I, Prot JC, Davide R (1996) Influence of water management on tolerance of rice cultivars for Meloidogyne graminicola. Fund Appl Nematol 19:189–192

    Google Scholar 

  • Thies JE, Rillig MC (2009) Characteristics of biochar: biological properties. Biochar Environ Manage: Sci Technology 1:85–105

    Google Scholar 

  • Tomati U, Grappelli A, Galli E (1987) The presence of growth regulators in earthworm-worked wastes. On Earthworms. In: Proceedings of International Symposium on Earthworms, Unione Zoologica Italiana, 2: 423–435.

  • Van Bezooijen J (2006) Methods and techniques for nematology. Wageningen University, Wageningen

    Google Scholar 

  • Wang C, Bruening G, Williamson VM (2009) Determination of preferred pH for root-knot nematode aggregation using pluronic F-127 gel. J Chem Ecol 35:1242–1251

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Waqas M, Khan AL, Kang SM, Kim YH, Lee IJ (2014) Phytohormone-producing fungal endophytes and hardwood-derived biochar interact to ameliorate heavy metal stress in soybeans. Biol Fert Soils 50:1155–1167

    Article  CAS  Google Scholar 

  • Waqas M, Kim YH, Khan AL, Shahzad R, Asaf S, Hamayun M, Kang SM, Khan MA, Lee IJ (2017) Additive effects due to biochar and endophyte application enable soybean to enhance nutrient uptake and modulate nutritional parameters. J Zhejiang Univ Sci B 18:109–124

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Waqas M, Shahzad R, Hamayun M, Asaf S, Khan AL, Kang SM, Yun S, Kim KM, Lee IJ (2018) Biochar amendment changes jasmonic acid levels in two rice varieties and alters their resistance to herbivory. PLoS ONE 13:1–18

    Article  Google Scholar 

  • Win PP, Kyi PP, Maung ZTZ, Myint YY, Cabasan MTN, De Waele D (2016) Host status of rotation crops in Asian rice-based cropping systems to the rice root-knot nematode Meloidogyne graminicola. Trop Plant Pathol 41:312–319

    Article  Google Scholar 

  • Xiao Z, Liu M, Jiang L, Chen X, Griffiths BS, Li H, Hu F (2016) Vermicompost increases defense against root-knot nematode (Meloidogyne incognita) in tomato plants. Appl Soil Ecol 105:177–186

    Article  Google Scholar 

  • Yasir M, Aslam Z, Kim SW, Lee SW, Jeon CO, Chung YR (2009) Bacterial community composition and chitinase gene diversity of vermicompost with antifungal activity. Bioresour Technol 100:4396–4403

    Article  CAS  PubMed  Google Scholar 

  • You X, Tojo M, Ching S, Wang KH (2018) Effects of vermicompost water extract prepared from bamboo and kudzu against Meloidogyne incognita and Rotylenchulus reniformis. J Nematol 50:1–10

    Article  Google Scholar 

  • Yuan JH, Xu RK (2012) Effects of biochars generated from crop residues on chemical properties of acid soils from tropical and subtropical China. Soil Res 50:570–578

    Article  CAS  Google Scholar 

  • Zhang XK, Qi L, Liang WJ, Zhang M, Xue-Lian B, Zu-Bin X (2013) Soil nematode response to biochar addition in a Chinese wheat field. Pedosphere 23:98–103

    Article  CAS  Google Scholar 

  • Zwart DC, Kim SH (2012) Biochar amendment increases resistance to stem lesions caused by Phytophthora spp. in tree seedlings. Hort Sci 47:1736–1740

    Google Scholar 

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Acknowledgements

This research was funded by the Indian Statistical Institute, Kolkata, India. We appreciate the help of Mr. Hari Shankar Saw, Mr. Suman Kisku and Mr. Dewson Basuki for their technical assistance.

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  1. Indian Statistical Institute, Giridih, Jharkhand, 815301, India

    Sandip Mondal, Saibal Ghosh & Abhishek Mukherjee

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  1. Sandip Mondal
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  2. Saibal Ghosh
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  3. Abhishek Mukherjee
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Conception, experimental design, data collection and analysis were performed by Sandip Mondal and Abhishek Mukherjee. Characterization of biochar and vermicompost was done by Saibal Ghosh. The manuscript was prepared by both Sandip Mondal and Abhishek Mukherjee. All authors read and approved the final manuscript.

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Correspondence to Abhishek Mukherjee.

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Mondal, S., Ghosh, S. & Mukherjee, A. Application of biochar and vermicompost against the rice root-knot nematode (Meloidogyne graminicola): an eco-friendly approach in nematode management. J Plant Dis Prot 128, 819–829 (2021). https://doi.org/10.1007/s41348-021-00433-2

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