Abstract
Bacillus species are often regarded as ideal biocontrol agents against plant pathogens, which can produce various antimicrobial peptides (AMPs). In this study, strain BU108 with good antimicrobial activity against Streptomyces scabiei was isolated from the rhizosphere soil of a potato field, which was then identified as Bacillus subtilis through 16S rDNA sequence analysis. An antimicrobial protein was purified by ammonium sulfate precipitation, ion exchange chromatography and ultrafiltration. The active sample showed a single band on SDS-PAGE gel, which was identified by mass spectrometry as partial fragments of gamma-glutamyl transpeptidase (GGT). To verify this new finding, three potential AMPs (KT20, IF20 and AE26) were predicted based on the sequence and structure of GGT, and the antimicrobial activity test confirmed two AMPs (KT20 and IF20). As GGT was involved in the metabolism of glutathione in former references, in this study, we reported the antimicrobial activity of its degradation fragments for the first time, which not only enriched the antimicrobial substance of Bacillus subtilis, but also provided a new choice for potato common scab biocontrol.
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The authors thank the reviewers, whose comments and suggestions helped us to improve this manuscript.
Funding
This study was financially supported by Heilongjiang Bayi Agricultural University Support Program for San Heng San Zong (ZRCQC201904) and Heilongjiang Bayi Agricultural University Experimental Demonstration Base Project in Jiu San Farm.
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Liu, Q., Shen, YR. & Yin, KD. The degradation fragments of gamma-glutamyl transpeptidase from Bacillus subtilis BU108 have antimicrobial activity against Streptomyces scabiei. J Plant Dis Prot 128, 279–285 (2021). https://doi.org/10.1007/s41348-020-00371-5
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DOI: https://doi.org/10.1007/s41348-020-00371-5