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The role of silicon fertilization in the synthesis of phenolic compounds on chestnut plants infected with P. cinnamomi and C. parasitica

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Abstract

Silicon is considered a beneficial nutrient for agricultural crops by conferred protection against diseases. The antifungal effect of the phenolic extracts from chestnut plants treated with 0, 5, 7.5 and 10 mM SiK® was tested in vitro. The results showed that the phenolic extracts from 10 mM SiK®-treated plants presented strongest inhibition zone against P. cinnamomi (10.56 cm2) and C. parasitica (11.2 cm2) in vitro assays at 8 days after incubation. The highest concentration of Si induced the increase in phenylalanine ammonia-lyase and polyphenol oxidase activity in chestnut plants infected with P. cinnamomi and C. parasitica. In addition, the phenolic extracts from Si-treated leaves were analyzed by high-performance liquid chromatography (HPLC–DAD) and the compounds identified were gallic acid, gallic acid derivate, vescalagin, castalagin, 1-O-Galloyl castalagin, chlorogenic acid, coumaric acid, syringic acid, vanillic acid, ellagic acid, quercitin-3-O-rutinoside and apigenin. The main phenolic compounds found in Si-treated plants (10 mM SiK®) were castalagin, gallic acid and its derivatives with an increase of 463%, 244% and 274%, respectively, when compared with untreated plants which can be correlated with the induction of resistance in chestnut plants against phytopathogenic agents studied. The present study provides the evident antifungal activity of the phenolic extracts from plants treated with concentrations of 7.5 and 10 mM SiK® in vitro assays suggesting that Si application can be useful in the future to control the ink disease and chestnut blight in this crop.

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References

  • Abe LT, Lajolo FM, Genovese MI (2010) Comparison of phenol content and antioxidant capacity of nuts. Ciênc Tecnol Aliment 30:254–259. https://doi.org/10.1590/S0101-20612010000500038

    Article  Google Scholar 

  • Abreu CG, Martins LM, Cardoso A, Borges O, Carvalho L, Gouveia EM (1999) Chestnut ink disease. An integrated approach to its control. In: Abreu CG (ed) NATO/science for stability programme. UTAD, Vila Rea, pp 43–53

    Google Scholar 

  • Ahmed T (2016) Comparative analysis of antifungal activity of total phenolics from different date palm cultivars against five phyto pathogenic fungi. In: Qatar foundation annual research conference proceedings

  • Aires A, Carvalho R, Rosa EAS, Saavedra MJ (2013) Phytochemical characterization and antioxidant properties of organic baby-leaf watercress produced under organic production system. CyTA J Food 11:343–351

    CAS  Google Scholar 

  • Alvarez J, Datnoff LE (2001) The economics of silicon for integrated management and sustainable production of rice and sugarcane. In: Datnoff LE, Snyder GH, Korndorfer GH (eds) Studies in plant science. Elsevier, Amsterdam, pp 221–239

    Google Scholar 

  • Ansari MA, Amurag A, Fatima Z, Hameed S (2013) Natural phenolic compounds: a potential antifungal agent. Microbiology 4(2):1189–1195

    Google Scholar 

  • Asanzi NM, Taylor NJ, Vahrmeijer JT (2014) Can silicon be used to prevent Alternaria alternata in citrus trees? SA Fruit J 13(7):48–51

    Google Scholar 

  • Bekker T, Aveling T, Kaiser C, Labuschagne N, Regnier T (2007) Accumulation of total phenolics due to silicon application in roots of avocado trees infected with Phytophthora cinnamomi. In: Proceedings VI world avocado congress

  • Bekker TF, Kaiser C, Labuschgne N (2009) The antifungal activity of potassium silicate and the role of Ph against selected plant pathogenic fungi in vitro. S Afr Plant Soil 26(1):55–57

    Google Scholar 

  • Bhatta D, Sharma G (2018) Role of silicon in counteracting abiotic and biotic plant stresses. Int J Chem Stud 6(2):1434–1442

    Google Scholar 

  • Bhattacharya A, Sood P, Citovsky V (2010) The roles of plant phenolics in defence and communication during Agrobacterium and Rhizobium infection. Mol Plant Pathol 11(5):705–719

    CAS  PubMed  PubMed Central  Google Scholar 

  • Brecht MO, Datnoff LE, Kucharek TA, Nagata RT (2004) Effect of silicon and chlorothalonil on suppression of gray leaf spot and increase plant growth in St. Augustinegrass. Plant Dis 88:338–344

    CAS  PubMed  Google Scholar 

  • Carneiro-Carvalho A, Pereira C, Marques T, Martins L, Anjos R, Pinto T, Lousada J, Gomes-Laranjo J (2017) Potential of silicon fertilization in the resistance of chestnut plants to ink disease (Phytophthora cinnamomi). Int J Environ Agric Biotechnol 2:2740–2753

    Google Scholar 

  • Chiarini A, Micucci M, Budriesi R, Ioan P, Leni M, Fimognari C, Toschi T, Comandini P, Hrelia S (2013) Sweet chestnut (Castanea sativa Mill.) bark extract: cardiovascular activity and myocyte protection against oxidative damage. Oxid Med Cell Longev. https://doi.org/10.1155/2013/471790

    Article  PubMed  PubMed Central  Google Scholar 

  • Chérif M, Asselin A, Bélanger RR (1994) Defense responses induced by soluble silicon in cucumber roots infected by Pythium spp. Phytopathology 84:236–242

    Google Scholar 

  • Chong KP, Atong M, Rossall S (2012) The role of syringic acid in the interaction between oil palm and Ganoderma boninense, the causal agent of basal stem rot. Plant Pathol 61:953–963

    CAS  Google Scholar 

  • Dallagnol LJ, Rodrigues FA, Pascholati SF, Fortunato AA, Camargo LEA (2015) Comparison of root and foliar applications of potassium silicate in potentiating post infection defences of melon against powdery mildew. Plant Pathol 64(5):1085–1093

    CAS  Google Scholar 

  • Dinis LT, Peixoto F, Zhang C, Martins L, Costa R, Gomes-Laranjo J (2011) Physiological and biochemical changes in resistant and sensitive chestnut (Castanea) plantlets after inoculation with Phytophthora cinnamomi. Physiol Mol Plant Pathol 75:146–156

    CAS  Google Scholar 

  • Dinis LT, Oliveira MM, Almeida J, Costa R, Gomes-Laranjo J, Peixoto F (2012) Antioxidant activities of chestnut nut of Castanea sativa Mill. (cultivar “Judia”) as function of origin ecosystem. Food Chem 132:1–8

    CAS  PubMed  Google Scholar 

  • Duan C, Yu J, Bai J, Zhu Z, Wang X (2014) Induced defense responses in rice plants against small brown planthopper infestation. Crop J 2:55–62

    Google Scholar 

  • Farahani L, Etebarian HR, Sahebani N, Aminian H (2012a) Biocontrol of blue mold of apple by Candida membranifaciens in combination with silicon. Arch Phytopathol Plant Protect 45(3):310–317

    Google Scholar 

  • Farahani L, Etevarian HR, Sahebani N, Aminian H (2012b) Effect of two strains of antagonistic yeasts in combination with silicon against two isolates of Penicillium expansum on apple fruit. Int J Appl Basic Sci 3(1):18–23

    CAS  Google Scholar 

  • Farooq MA, Ali S, Hameed A, Ishaque W, Mahmood K, Igbai Z (2013) Alleviation of cadmium toxicity by silicon is related to elevated photosynthesis, antioxidant enzymes, suppressed cadmium uptake and oxidative stress in cotton. Ecotoxicol Environ Saf 96:242–249. https://doi.org/10.1016/j.ecoenv.2013.07.006

    Article  CAS  PubMed  Google Scholar 

  • Fauteaux F, Rémus-Borel W, Menzies JG, Bélanger RR (2005) Silicon and plant disease resistance against pathogenic fungi. FEMS Microbiol Lett 249:1–6

    Google Scholar 

  • Fortunato AA, Rodrigues FA, Barone JCP, Soares GCB, Rodriguez MAD, Pereira OL (2012) Silicon suppresses Fusarium wilt development in banana plants. J Phytopathol 160:674–679

    CAS  Google Scholar 

  • Fortunato AA, Silva WL, Rodrigues FA (2014) Phenylpropanoid pathway is potentiated by silicon in the roots of banana plants during the infection process of Fusarium oxysporum f. sp. Cubense. Phytopathology 104:597–603

    CAS  PubMed  Google Scholar 

  • Gatto MA, Sanzani SM, Tardia P, Linsalata V, Pieralice M, Sergio L, Venere DD (2013) Antifungal activity of total and fractionated phenolic extracts from two wild edible herbs. Nat Sci 5:895–902

    Google Scholar 

  • Habibi G, Hajiboland R (2010) Alleviation of drought stress by silicon supplementation in pistachio (Pistacia vera L.) plants. Folia Hortic 25(1):21–29

    Google Scholar 

  • Han Y, Li P, Gong S, Yang L, Wen L, Hou M (2016) Defense responses in rice induced by silicon amendment against infestation by the leaf folder Cnaphalocrocis medinalis. PLoS ONE 11:1–14

    Google Scholar 

  • Harizanova A, Zlatev Z, Koleva L (2014) Effect of silicon on activity of antioxidant enzymes and photosynthesis in leaves of cucumber plants (Cucumis sativus L.). Turk J Agric Nat Sci 2:1812–1817

    Google Scholar 

  • Kulbat K (2016) The role of phenolic compounds in plant resistance. Biotechnol Food Sci 80(2):97–108

    Google Scholar 

  • Kumar L, Mahatma MK, Kalariya KA, Bishi SK, Mann A (2014) Plant phenolics: important bio-weapon again pathogens and insect herbivores. Popular Kheti 2(3):149–152

    Google Scholar 

  • Lattanzio V, Lattanzio VM, Cardinali A (2006) Role of phenolics in the resistance mechanisms of plants against fungal pathogens and insects. Phytochem Adv Res 661(2):23–67

    Google Scholar 

  • Lin LZ, Harnly JM (2007) A screening method for the identification of glycosylated flavonoids and other phenolic compounds using standard analytical approach for all plant materials. J Agric Food Chem 55:1084–1096

    CAS  PubMed  PubMed Central  Google Scholar 

  • Lu G, Jian W, Zhang J, Zhou Y, Cao J (2008) Suppressive effect of silicon nutrient on phomopsis stem blight development in asparagus. Am Soc Hortic Sci 43:811–817

    Google Scholar 

  • Mandal S, Kar I, Mukherjee AK, Acharya P (2013) Elicitor-induced defense responses in Solanum lycopersicum against Ralstonia solanacearum. Sci World J. https://doi.org/10.1155/2013/561056

    Article  Google Scholar 

  • Mazid M, Khan TA, Mohammad F (2011) Role of secondary metabolties in defense mechanisms of plants. Biol Med 3(2):232–249

    CAS  Google Scholar 

  • Mendes LS, Souza CHE, Machado VJ (2011) Fertilization with silicon: influence on soil, plant, pests and pathogens. Cerrado Agrociências 2:51–63

    Google Scholar 

  • Moura L, Pereira G, Costa SR, Martins M, Gouveia E (2015) Cancro do castanheiro na região do Minho: estrutura populacional e variabilidade genética de populações de Cryphonectria parasitica. Revista de Ciências Agrárias 38:238–247

    Google Scholar 

  • Mujic I, Dudas S, Zivkovic J, Tomin J, Zekovic Z, Alibabié V (2009) Determination of total phenolic compounds in cultivars of Castanea sativa Mill. Acta Hort 815:63–68

    CAS  Google Scholar 

  • Ojha S, Chatterjee NC (2012) Induction of resistance in tomato plants against fusarium oxysporum F. sp. Lycopersici mediated thorough salicylic acid and Trichoderma Harzianum. J Plant Protec Res 52:220–225

    CAS  Google Scholar 

  • Oliveira JC, Albuquerque GMR, Mariano RLR, Gondim DMF, Oliveira JTA, Souza EB (2012) Reduction of the severity of angular leaf spot of cotton mediated by silicon. J Plant Pathol 94(2):297–304

    Google Scholar 

  • Polanco RL, Rodrigues FA, Nascimento JTK, Cruz MFA, Curvelo CRS, Da Matta FM, Vale FXR (2014) Photosynthetic gas exchange and antioxidative system in common bean plants infected by Colletotrichum lindemuthianum and supplied with silicon. Trop Plant Pathol 39:35–42

    Google Scholar 

  • Purwar S, Gupta SM, Kumar A (2012) Enzymes of Phenylpropanoid metabolism involved in strengthening the structural barrier for providing genotype and stage dependent resistance to karnal bunt in wheat. Am J Plant Sci 3:261–267

    CAS  Google Scholar 

  • Qin GZ, Shi PT (2005) Enhancement of biocontrol activity of Cryptococcus laurentii by silicon and the possible mechanisms involved. Biol Control 95(1):69–75

    Google Scholar 

  • Rahman A, Wallis CM, Uddin W (2015) Silicon-induced systemic defense responses in perennial ryegrass against infection by Magnaporthe oryzae. Phytopathology 105:748–757. https://doi.org/10.1094/PHYTO-12-14-0378-R

    Article  CAS  PubMed  Google Scholar 

  • Rani PU, Jyothsna Y (2010) Biochemical and enzymatic changes in rice plants as a mechanism of defense. Acta Physiol Plant 32(4):695–701

    CAS  Google Scholar 

  • Reinoso BD, Couto D, Moure A, Fernandes E, Domínguez H, Parajo JC (2012) Optimization of antioxidants—extraction from Castanea sativa leaves. Chem Eng J 203:101–109

    Google Scholar 

  • Rigling D, Prospero S (2018) Cryphonectria parasitica, the causal agent of chestnut blight: invasion history, population biology and disease control. Molec Plant Pathol 19(1):7–20

    CAS  Google Scholar 

  • Rongai D, Pulcini P, Pesce B, Milano F (2015) Antifungal activity of some botanical extracts on Fusarium oxysporum. Open Life 10:409–416

    CAS  Google Scholar 

  • Sakakibara H, Honda Y, Nakagawa S, Ashida H, Kanazawa K (2003) Simultaneously determination of all polyphenols in vegetables, fruits and teas. J Agric Food Chem 51:571–581

    CAS  PubMed  Google Scholar 

  • Schabl P (2014) Silica application as a promising approach for control of fungal diseases for grapevine Vitis vinifera L. Master Thesis, Hochschule Geisenheim University, p 121

  • Schulenburg K, Feller A, Hoffman T, Schecker JH, Martens S, Schwab W (2016) Formation of β-glucogallin, the precursor of ellagic acid in strawberry and raspberry. J Exp Bot 67(8):2299–2308

    CAS  PubMed  PubMed Central  Google Scholar 

  • Schurt DA, Cruz MFA, Nascimento KJT, Filippi MCC, Rodrigues FA (2014) Silicon potentiates the activities of defense enzymes in the leaf sheaths of rice plants infected by Rhizoctonia solani. Trop Plant Pathol 39:457–463

    Google Scholar 

  • Shen GH, Xue QH, Tang M, Chen Q, Wang LN, Duan CM, Xue L, Zhao J (2010) Inhibitory effects of potassium silicate on five soil-borne phytopathogenic fungi in vitro. J Plant Dis Prot 117(4):180–184

    CAS  Google Scholar 

  • Shetty R, Fretté X, Jensen B, Shetty NP, Jensen JD, Jorgensen HJL et al (2011) Silicon-induced changes in antifungal phenolic acids, flavonoids and key phenylpropanoid pathway genes, during the interaction between miniature roses and the biotrophic pathogen Podosphaera pannosa. Plant Physiol 157:2194–2205

    CAS  PubMed  PubMed Central  Google Scholar 

  • Siddique Z, Akhtar KP, Hameed A, Sarwar N, Ul-Haq I, Khan SA (2014) Biochemical alterations in leaves of resistant and susceptible cotton genotypes infected systemically by cotton leaf curl Burewala virus. J Plant Interact 9:702–711

    Google Scholar 

  • Silva JC, Duarte IN, Moura EAC, Coelho L (2011) Crescimento de Cylindrocladium sp. e Botrytis cinerea em meio de cultivo com silício. Encicliopédia Biosfera 6(11):1–6

    Google Scholar 

  • Singleton VL, Rossi JAJ (1965) Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic 16:144–158

    CAS  Google Scholar 

  • Slatnar A, Mikulic-Petkovsek M, Veberic R, Stampar F (2016) Research on the involvment of phenolics in the defense of horticultural plants. Acta Agric Slov 107(1):183–189

    CAS  Google Scholar 

  • Song A, Xue G, Cui P, Fan F, Liu H, Yin C, Sun W, Liang Y (2016) The role of silicon in enhancing resistance to bacterial blight of hydroponic-and soil-cultured rice. Sci Rep 6:24640

    CAS  PubMed  PubMed Central  Google Scholar 

  • Spáčil Z, Nováková L, Solich P (2008) Analysis of phenolic compounds by high performance liquid chromatography and ultra-performance liquid chromatography. Talanta 76:189–199

    PubMed  Google Scholar 

  • Thakur M, Sohal S (2013) Role of elicitors in inducing resistance in plants against pathogen infection: a review. ISRN Biochem. https://doi.org/10.1155/2013/762412

    Article  PubMed  PubMed Central  Google Scholar 

  • Van Bockhaven J, Vleesschauwer DD, Höfte M (2013) Towards establishing broad-spectrum diseases resistance in plants: silicon leads the way. J Exp Bot 64(5):1281–1293

    PubMed  Google Scholar 

  • Vannini A, Vettraino AM (2001) Ink disease in chestnuts: impact on the European chestnut. For Snow Landsc Res 76(3):345–350

    Google Scholar 

  • Vasconcelos MC, Bennett R, Rosa E, Ferreira-Cardoso JV (2010) Composition of European chestnut (Castanea sativa Mill.) and association with health effects: fresh and processed products. J Sci Food Agric 90:1578–1589

    PubMed  Google Scholar 

  • Vivancos J, Labbé C, Menzies JG, Bélanger RR (2015) Silicon-mediated resistance of Arabidopsis against powdery mildew involves mechanisms other than the salicylic acid (SA)-dependent defense pathway. Mol Plant Pathol 16:572–582

    CAS  PubMed  Google Scholar 

  • Wang H, Provan GJ, Helliwell K (2003) HPLC determination of catechins in tea leaves and tea extracts using relative response factors. Food Chem 81:307–312

    CAS  Google Scholar 

  • Wang M, Gao L, Dong S, Sun Y, Shen Q, Guo S (2017) Role of silicon on plant-pathogen interactions. Front Plant Sci. https://doi.org/10.3389/fpls.2017.00701

    Article  PubMed  PubMed Central  Google Scholar 

  • Whan JA, Dann EK, Aitken AB (2016) Effects of silicon treatment and inoculation with Fusarium oxysporum f. sp. vasinfectum on cellular defences in root tissues of two cotton cultivars. Ann Bot 118:219–226

    CAS  PubMed  PubMed Central  Google Scholar 

  • Yang L, Han Y, Li P, Li F, Ali S, Hou M (2017) Silicon amendment is involved in the induction of plant defense responses to a phloem feeder. Sci Rep 7(4232):1–9

    Google Scholar 

  • Zhang G, Cui Y, Ding X, Dai Q (2013) Stimulation of phenolic metabolism by silicon contributes to rice resistance to sheath blight. J Plant Nutr Soil Sci 176(1):118–124

    CAS  Google Scholar 

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Acknowledgements

This work was supported by National Funds by FCT—Portuguese Foundation for Science and Technology, under the Project UID/AGR/04033/2019.

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Authors and Affiliations

  1. Biology and Environment Department, Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Trás-os-Montes and Alto Douro, 5000-801, Vila Real, Portugal

    Andreia Carneiro-Carvalho, Alfredo Aires, Rosário Anjos, Teresa Pinto & José Gomes-Laranjo

  2. Forest Sciences and Landscape Architecture Department (CIFAP), University of Trás-os-Montes and Alto Douro, 5000-801, Vila Real, Portugal

    Luís Martins

  3. Vila Real Chemistry Center (CQVR), University of Trás-os-Montes and Alto Douro, 5000-801, Vila Real, Portugal

    Francisco Peixoto

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  1. Andreia Carneiro-Carvalho
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Carneiro-Carvalho, A., Aires, A., Anjos, R. et al. The role of silicon fertilization in the synthesis of phenolic compounds on chestnut plants infected with P. cinnamomi and C. parasitica. J Plant Dis Prot 127, 211–227 (2020). https://doi.org/10.1007/s41348-019-00292-y

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