Journal of Plant Diseases and Protection

, Volume 125, Issue 3, pp 331–337 | Cite as

Real-time assays for detection of Phytophthora spp. and identification of an avr3a gene variant

  • Shimaa R. T. Tolba
  • Mariella M. Finetti Sialer
  • Laura C. Rosso
  • Mahmoud M. A. Moustafa
  • Chiara Ruggeri
  • Ibrahim I. S. El-Shawaf
  • Aurelio Ciancio
Short Communication

Abstract

A study was carried out for identification and detection of Phytophthora spp. from soil and plant samples, collected from Solanaceous crops in Egypt and Italy. The samples were screened with specific and universal primers of Phytophthora 18S, ITS1–ITS2 and 28S regions, followed by PCR product sequencing. The Phytophthora spp. detected were P. infestans (Egypt) and P. parasitica (Italy). A molecular beacon probe was also developed based on the avr3a gene of P. infestans to detect a variant associated with virulence traits. The probe was suitable for avr3a allele identification from P. infestans and also from P. parasitica PCR products.

Keywords

avr3a ITS Molecular beacon Phytophthora infestans Phytophthora parasitica Virulence 

Notes

Acknowledgements

Research developed as part of STT higher degree requirements. Study partially funded by the EU Commission, Joint Master Degree Tempus Project No. 543865. The authors thank prof. Sanaa Haroon (Fayum University, Egypt) for travel assistance and coordination, and Dr Michelina Ruocco, IPSP CNR, Portici, for suggestions and isolate CBS 120920.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Human and animal rights

Research does not involve human participants and/or animals.

Informed consent

All authors gave informed consent to publish the manuscript.

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Copyright information

© Deutsche Phytomedizinische Gesellschaft 2018

Authors and Affiliations

  1. 1.Department of Genetics and Genetic Engineering, Faculty of AgricultureBenha UniversityMoshtohorEgypt
  2. 2.Consiglio Nazionale delle Ricerche, Istituto di Bioscienze e BiorisorseBariItaly
  3. 3.Consiglio Nazionale delle Ricerche, Istituto per la Protezione Sostenibile delle PianteBariItaly

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