Journal of Plant Diseases and Protection

, Volume 125, Issue 2, pp 205–212 | Cite as

Distribution of Alternaria species on blighted potato and tomato leaves in Russia

  • Lyudmila Y. Kokaeva
  • Arseniy F. Belosokhov
  • Larisa Y. Doeva
  • Ekaterina S. Skolotneva
  • Sergey N. Elansky
Original Article


Alternaria species are the causal agents of potato and tomato early blight disease. Three species-specific PCR primer sets were designed for Alternaria alternata sensu lato, Alternaria solani and Alternaria infectoria identification. The specificity of primers was confirmed by the absence of amplified products after PCR with DNA of Alternaria spp., other potato and tomato pathogenic fungal species and plant DNA. Constructed primers were applied for the survey of early blight agents on affected potato and tomato leaves collected in different regions of Russia. All three Alternaria pathogens were present in blighted leaves alone or in complex (A. alternata + A. solani, A. infectoria + A. alternata and A. solani + A. infectoria). Alternaria alternata (sensu lato) was the most abundant species. It was detected in 50% of tested potato leaves and 41% of tested tomato leaves (alone or in complex). Alternaria solani was found in 30 and 35% leaves, respectively. Alternaria infectoria occurred in potato and tomato samples occurred at a somewhat lower frequency (13 and 8%, respectively). Using the specific primers, it was possible to exclude the Alternaria disease in some leaves with symptoms similar to early blight.


Early blight Alternaria sp. Leaf blight Potato diseases Tomato diseases 



Financial support to the work with collected materials and fungal isolates was provided by the Russian Science Foundation (Project No. 14-50-00029). Ekaterina Skolotneva was supported by the State Budget Project (No. 0324-2016-0001); Lyudmila Kokaeva was supported by the Foundation for Assistance to Small Innovative Enterprises in Science and Technology (FASIE, Project No. 10566GU/2016).

Compliance with ethical standards

Conflict of interest

All authors declare that they have no conflict of interest.


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Copyright information

© Deutsche Phytomedizinische Gesellschaft 2017

Authors and Affiliations

  1. 1.Faculty of BiologyLomonosov Moscow State UniversityMoscowRussia
  2. 2.North Caucasus Research Institute of Mountain and Submountane AgricultureVladikavkazRussia
  3. 3.Institute of Cytology and Genetics SB RASNovosibirskRussia

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