Journal of Plant Diseases and Protection

, Volume 125, Issue 2, pp 205–212 | Cite as

Distribution of Alternaria species on blighted potato and tomato leaves in Russia

  • Lyudmila Y. Kokaeva
  • Arseniy F. Belosokhov
  • Larisa Y. Doeva
  • Ekaterina S. Skolotneva
  • Sergey N. Elansky
Original Article
  • 86 Downloads

Abstract

Alternaria species are the causal agents of potato and tomato early blight disease. Three species-specific PCR primer sets were designed for Alternaria alternata sensu lato, Alternaria solani and Alternaria infectoria identification. The specificity of primers was confirmed by the absence of amplified products after PCR with DNA of Alternaria spp., other potato and tomato pathogenic fungal species and plant DNA. Constructed primers were applied for the survey of early blight agents on affected potato and tomato leaves collected in different regions of Russia. All three Alternaria pathogens were present in blighted leaves alone or in complex (A. alternata + A. solani, A. infectoria + A. alternata and A. solani + A. infectoria). Alternaria alternata (sensu lato) was the most abundant species. It was detected in 50% of tested potato leaves and 41% of tested tomato leaves (alone or in complex). Alternaria solani was found in 30 and 35% leaves, respectively. Alternaria infectoria occurred in potato and tomato samples occurred at a somewhat lower frequency (13 and 8%, respectively). Using the specific primers, it was possible to exclude the Alternaria disease in some leaves with symptoms similar to early blight.

Keywords

Early blight Alternaria sp. Leaf blight Potato diseases Tomato diseases 

Notes

Acknowledgements

Financial support to the work with collected materials and fungal isolates was provided by the Russian Science Foundation (Project No. 14-50-00029). Ekaterina Skolotneva was supported by the State Budget Project (No. 0324-2016-0001); Lyudmila Kokaeva was supported by the Foundation for Assistance to Small Innovative Enterprises in Science and Technology (FASIE, Project No. 10566GU/2016).

Compliance with ethical standards

Conflict of interest

All authors declare that they have no conflict of interest.

References

  1. Andersen B, Thrane U (1996) Differentiation of Altemaria infectoria and Alternaria alternata based on morphology, metabolite profiles, and cultural characteristics. Can J Microbiol 42(7):685–689CrossRefGoogle Scholar
  2. Ardestani ST, Sharifnabi B, Zare R, Moghadam AA (2010) New Alternaria species associated with potato leaf spot in various potato growing regions of Iran. Iran J Plant Pathol 45(4):83–86Google Scholar
  3. Babler E, Asensio N, Leiminger J, Hausladen H, Bahnweg G, Zinkernagel V (2004) Investigations on the appearance and control of Alternaria solani and Alternaria alternata on potato. Mitteilungen der Biologischen Bundesanstalt f. Land- & Forstwirtschaft 396:100–101Google Scholar
  4. Dang HX, Pryor B, Peever T, Lawrence CB (2015) The Alternaria genomes database: a comprehensive resource for a fungal genus comprised of saprophytes, plant pathogens, and allergenic species. BMC Genom 16(1):239CrossRefGoogle Scholar
  5. Droby S, Dinoor A, Prusky D, Barkai-Golan R (1984) Pathogenicity of Alternaria alternata on potato in Israel. Phytopathology 74:537–542. doi: 10.1094/Phyto-74-537 CrossRefGoogle Scholar
  6. Elansky S, Pobedinskaya M, Kokaeva L, Statsyuk N, Alexandrova A (2012) Molecular identification of the species composition of Russian isolates of pathogens, causing early blight of potato and tomato. In: Euroblight. PPO-special report, vol 15. Wageningen University Lelystad, The Netherland, pp 151–156Google Scholar
  7. Gannibal PB, Yli-Mattila T (2007) Morphological and UP-PCR analyses and design of a PCR assay for differentiation of Alternaria infectoria species-group. Mikologiya i fitopatologiia 41(4):313Google Scholar
  8. Griffith GW, Shaw DS (1998) Polymorphisms in Phytophthora infestans: four mitochondrial haplotypes are detected after PCR amplification of DNA from pure cultures or from host lesions. Appl Environ Microbiol 64(10):4007–4014PubMedPubMedCentralGoogle Scholar
  9. Kapsa JS (2008) Important threats in potato production and integrated pathogen/pest management. Potato Res 51(3–4):385–401CrossRefGoogle Scholar
  10. Kokaeva LY, Kudryavtzeva NN, Pobedinskaya MA, Zaitchik BT, Elansky SN (2015) Virulence of Alternaria alternata strains isolated from different organs of host plants toward different potato and tomato cultivars. Zasita kartofela 1:14–18Google Scholar
  11. Kudryavtzeva NN, Pobedinskaya MA, Kokaeva LY, Zaitchik BT, Kutuzova IA, Azarkovich MI, Elansky SN, Valueva TA (2017) The proteolytic activity and virulence of Alternaria alternata strains, isolated from tomato. Mikologiya I Fitopatologiya 51(2):110–116Google Scholar
  12. Landschoot S, Vandecasteele M, Carrette J, De Baets B, Hцfte M, Audenaert K, Haesaert G (2017) Assessing the Belgian potato Alternaria population for sensitivity to fungicides with diverse modes of action. Eur J Plant Pathol 148:657–672. doi: 10.1007/s10658-016-1123-3 CrossRefGoogle Scholar
  13. Latorse MP, Schmitt F, Peyrard S, Veloso S, Beffa R (2010) Molecular analysis of Alternaria populations early blight causal agents in potato plants. PPO Spec Rep 14:179–186Google Scholar
  14. Leiminger J, Hausladen H (2012) Wirkung verschiedener Fungizide auf den Befall der Dьrrfleckenkrankheit (Alternaria spp.) sowie auf den Ertrag der Kartoffel. Gesunde Pflanzen 63:11–18CrossRefGoogle Scholar
  15. Leiminger J, Hausladen H (2013) Fungizidresistenz bei Alternaria. Kartoffelbau 5:18–21Google Scholar
  16. Leiminger J, Bahnweg G, Hausladen H (2014) Differentiation of Alternaria species and quantification of disease development using real-time PCR. In: Euroblight. PPO-Special Report, vol 16. Wageningen University Lelystad, The Netherland, pp 189–194Google Scholar
  17. Orina AS, Gannibal PB, Levitin MM (2010) Species diversity, biological characters and geography of Alternaria fungi associated with solanaceous plants. Mikologiya I Fitopatologiya 44:150–159Google Scholar
  18. Pavyn M, Gonzбlez I, Rojas M, Pegels N, Martнn R, Garcнa T (2011) PCR detection of Alternaria spp. in processed foods, based on the internal transcribed spacer genetic marker. J Food Prot 74(2):240–247CrossRefGoogle Scholar
  19. Pobedinskaya MA, Plutalov PN, Romanova SS, Kokaeva LY, Nikolaev AV, Alexandrova AV, Elansky SN (2012) Resistance of potato and tomato early blight pathogens to fungicides. Mikologiya I Fitopatologiya 46(6):402–408Google Scholar
  20. Rodrigues TTMS, Berbee ML, Simmons EG, Cardoso CR, Reis A, Maffia LA, Mizubuti ESG (2010) First report of Alternaria tomatophila and A. grandis causing early blight on tomato and potato in Brazil. New Dis Rep 22:28CrossRefGoogle Scholar
  21. Saharan GS, Mehta N, Meena PD, Dayal P (2016) Alternaria diseases of crucifers: biology, ecology and disease management. Springer, SingaporeCrossRefGoogle Scholar
  22. Turkensteen J, Spoelder J, Mulder A (2010) With the real Alternaria stand up please—experiences with Alternaria-like diseases on potatoes during the 2009 season in the Netherlands. In: Euroblight. PPO-Special Report, vol 14. Wageningen University Lelystad, The Netherland, pp 165–170. In: Schepers HTAM (2010) PPO-special report no. 14. DLO Foundation, Wageningen, pp 165–170Google Scholar
  23. Tymon LS, Johnson DA (2014) Fungicide resistance of two species of Alternaria from potato in the Columbia Basin of Washington. Plant Dis 98:1648–1653CrossRefGoogle Scholar
  24. Tymon LS, Peever TL, Johnson DA (2016) Identification and enumeration of small-spored Alternaria species associated with potato in the U.S. Northwest Plant Dis 100:465–472CrossRefGoogle Scholar
  25. White TJ, Bruns T, Lee SJWT, Taylor JW (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR Protoc Guide Methods Appl 18(1):315–322Google Scholar
  26. Woudenberg JHC, Seidl MF, Groenewald JZ, de Vries M, Stielow JB, Thomma BPHJ, Crous PW (2015) Alternaria section Alternaria: species, formae speciales or pathotypes? Stud Mycol. doi: 10.1016/j.simyco.2015.07.001 PubMedPubMedCentralGoogle Scholar
  27. Zheng HH, Zhao J, Wang TY, Wu XH (2015) Characterization of Alternaria species associated with potato foliar diseases in China. Plant Pathol 64(2):425–433CrossRefGoogle Scholar

Copyright information

© Deutsche Phytomedizinische Gesellschaft 2017

Authors and Affiliations

  1. 1.Faculty of BiologyLomonosov Moscow State UniversityMoscowRussia
  2. 2.North Caucasus Research Institute of Mountain and Submountane AgricultureVladikavkazRussia
  3. 3.Institute of Cytology and Genetics SB RASNovosibirskRussia

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