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The promotion of thiophanate-methyl and tebuconazole for the continuous control of Glomerella leaf spot in apple leaves by adding pellouxite as a synergistic reagent

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Abstract

Some site-specific fungicides were usually mixed with synergistic agrochemicals to prolong the active life and lower the risk of the resistance. As an important composition in Bordeaux mixture, pellouxite is viscous and may help fungicides increase the efficacy and lower the risk of resistance. The aim of this work was to investigate the effects of pellouxite on the efficacies and lasting periods of thiophanate-methyl (TM) and tebuconazole (TEB) for the control of Glomerella leaf spot (GLS) in apple leaves. Fungicide efficacy was determined by the detached leaf inoculation method. Results showed adding pellouxite increased the efficacies of TM and TEB with the increase rates ≥33% at 21 and 28 days after treatment. Additional analysis suggested there was a synergistic response in the control of apple GLS when TM or TEB was mixed with pellouxite. To illustrate this effect, the fungicide residue dynamic in apple leaves and the sensitivity of Glomerella cingulata to fungicides were investigated by the UPLC–MS/MS and mycelia linear growth rate method, respectively. Results showed adding pellouxite increased the DT50 values for the active metabolite of TM as well as TEB and also lowered their EC50 values. These suggested adding pellouxite increased their lasting periods and also enhanced their inhibitory effects on the growth of G. cingulata. In conclusion, adding pellouxite to two fungicides can increase and prolong their efficacy, which may lower their application rate and also the risk of the resistance.

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References

  • Chen, H., Liu, X., Wang, C., Wang, Q., Jiang, Y., Yin, P., et al. (2013). Simultaneous determination of thiophanate-methyl and its metabolite carbendazim in tea using isotope dilution ultra performance liquid chromatography-tandem mass spectrometry. Journal of Chromatographic Science, 1093, 1–8.

    Google Scholar 

  • Colby, S. R. (1967). Calculating synergistic and antagonistic responses of herbicide combinations. Weeds, 15, 20–22.

    Article  CAS  Google Scholar 

  • Cromey, M., Butler, R., Mace, M., & Cole, A. (2004). Effects of the fungicides azoxystrobin and tebuconazole on Didymella exitialis, leaf senescence and grain yield in wheat. Crop Protection, 23, 1019–1030.

    Article  CAS  Google Scholar 

  • Cycoń, M., Wójcik, M., & Piotrowska-Seget, Z. (2011). Biodegradation kinetics of the benzimidazole fungicide thiophanate-methyl by bacteria isolated from loamy sand soil. Biodegradation, 22, 573–583.

    Article  PubMed  Google Scholar 

  • Dias, M., Jesus, C., Pinto, G., Santos, C., Goncalves, B., Goncalves, D., et al. (2013). The role of kaolin and Bordeaux mixture in Vitis vinifera under water stress conditions: Oxidative stress. Journal of Environmental Biology, 94, 156–202.

    Google Scholar 

  • Dong, J., Luo, L., Wang, C., Leng, W., Li, G., & Li, B. (2009). Isolating strongly parasitic fungi by single-spore isolation aided with capillary stiletto. Chinese Agricultural Science Bulletin, 3, 46. (in chinese).

    Google Scholar 

  • Fisher, M. C., Henk, D. A., Briggs, C. J., Brownstein, J. S., Madoff, L. C., McCraw, S. L., et al. (2012). Emerging fungal threats to animal, plant and ecosystem health. Nature, 484, 186–194.

    Article  CAS  PubMed  Google Scholar 

  • Gisi, U., & Sierotzki, H. (2008). Fungicide modes of action and resistance in downy mildews. European Journal of Plant Pathology, 122, 157–167.

    Article  CAS  Google Scholar 

  • Holb, I. J. (2009). Fungal disease management in environmentally friendly apple production-a review. In E. Lichtfouse (Ed.), Climate change, intercropping, pest control and beneficial microorganisms (pp. 219–292). Dordrecht: Springer Netherlands.

  • Hollomon, D. W. (2015). Fungicide resistance: Facing the challenge. Plant Protection Science, 51, 170–176.

    Article  Google Scholar 

  • Horsfall, J. G., & Cowling, E. B. (Eds.). (1978). How plants suffer from disease. In Plant disease, an advanced treatise. New York, USA: Academic Press.

    Google Scholar 

  • Ishii, H. (2004). Studies on fungicide resistance in phytopathogenic fungi. Journal of General Plant Pathology, 70, 379–381.

    Article  Google Scholar 

  • Jung, S. M., Nam, J. C., Huh, Y. Y., Roh, J. H., Park, K. S., & Lim, T. J. (2013). Effect of bordeaux mixture spray on fruit quality of ‘kyoho’ grape and copper accumulation in the soil. Acta Horticulture, 1001, 259–263.

    Article  Google Scholar 

  • Kim, Y. C., Lee, J. H., Bae, Y. S., Sohn, B. K., & Park, S. K. (2010). Development of effective environmentally-friendly approaches to control Alternaria blight and anthracnose diseases of Korean ginseng. European Journal of Plant Pathology, 127, 443–450.

    Article  Google Scholar 

  • Krämer, W., Schirmer, U., Jeschke, P., & Witschel, M. (2012). FRAC mode of action classification and resistance risk of fungicides. In K. H. Kuck, A. Leadbeater & U. Gisi (Eds.), Modern crop protection compounds (pp. 415–432). Weinheim, Germany: Wiley.

  • Li, Y., Dong, F., Liu, X., Xu, J., Li, J., Kong, Z., et al. (2012). Simultaneous enantioselective determination of triazole fungicides in soil and water by chiral liquid chromatography/tandem mass spectrometry. Journal of Chromatography A, 1224, 51–60.

    Article  CAS  PubMed  Google Scholar 

  • Mikaberidze, A., Mcdonald, B. A., & Bonhoeffer, S. (2013). Can high-risk fungicides be used in mixtures without selecting for fungicide resistance? Phytopathology, 104, 324–331.

    Article  Google Scholar 

  • Mohanty, B., Datta, P., Dasgupta, B., & Sengupta, D. K. (2011). Integrated management of foot and leaf rot of betelvine. SAARC Journal of Agriculture, 9, 83–91.

    Google Scholar 

  • Mueller, D., Bradley, C., Grau, C., Gaska, J., Kurle, J., & Pedersen, W. (2004). Application of thiophanate-methyl at different host growth stages for management of Sclerotinia stem rot in soybean. Crop Protection, 23, 983–988.

    Article  CAS  Google Scholar 

  • Niell, S., Cesio, V., Hepperle, J., Doerk, D., Kirsch, L., Kolberg, D., et al. (2014). QuEChERS-based method for the multiresidue analysis of pesticides in beeswax by LC-MS/MS and GC × GC-TOF. Journal of Agricultural and Food Chemistry, 62, 3675–3683.

    Article  CAS  PubMed  Google Scholar 

  • Soeda, Y., Kosaka, S., & Noguchi, T. (1972). The fate of thiophanate-methyl fungicide and its metabolites on plant leaves and glass plates. Agricultural and Biological Chemistry, 36, 931–936.

    Article  CAS  Google Scholar 

  • Stüpp, J. J., de Souza Gonçalves, P. A., & Boff, M. I. C. (2012). Bordeaux mixture and Diabrotica speciosa adults extract effect on plant sanitary management in common bean grown under organic crop system. Revista de Ciências Agroveterinárias, 11, 222–229.

    Google Scholar 

  • Swingle, W. T. (1896). Bordeaux mixture: Its chemistry, physical properties, and toxic effects on fungi and algae. Washington, DC: US Government Printing Office.

    Google Scholar 

  • Utture, S. C., Banerjee, K., Dasgupta, S., Patil, S. H., Jadhav, M. R., Wagh, S. S., et al. (2011). Dissipation and distribution behavior of azoxystrobin, carbendazim, and difenoconazole in pomegranate fruits. Journal of Agricultural and Food Chemistry, 59, 7866–7873.

    Article  CAS  PubMed  Google Scholar 

  • van den Bosch, F., Fraaije, B., Oliver, R., van den Berg, F., & Paveley, N. (2015). The use of mathematical models to guide fungicide resistance management decisions. In H. Ishii & D. W. Hollomon (Eds.), Fungicide resistance in plant pathogens (pp. 49–62). Tokyo: Springer.

  • van den Bosch, F., Oliver, R., van den Berg, F., & Paveley, N. (2013). Governing principles can guide fungicide-resistance management tactics. Annual review of Phytopathology, 52, 175–195.

    Article  Google Scholar 

  • van den Bosch, F., Paveley, N., Shaw, M., Hobbelen, P., & Oliver, R. (2011). The dose rate debate: Does the risk of fungicide resistance increase or decrease with dose? Plant Pathology, 60, 597–606.

    Article  Google Scholar 

  • van den Bosch, F., Paveley, N., van den Berg, F., Hobbelen, P., & Oliver, R. (2014). Mixtures as a fungicide resistance management tactic. Phytopathology, 104, 1264–1273.

    Article  PubMed  Google Scholar 

  • Veneziano, A., Vacca, G., Arana, S., De Simone, F., & Rastrelli, L. (2004). Determination of carbendazim, thiabendazole and thiophanate-methyl in banana (Musa acuminata) samples imported to Italy. Food Chemistry, 87, 383–386.

    Article  CAS  Google Scholar 

  • Wang, B., Li, B., Dong, X., Wang, C., & Zhang, Z. (2015). Effects of temperature, wetness duration, and moisture on the conidial germination, infection, and disease incubation period of Glomerella cingulata. Plant Disease, 99, 249–256.

    Article  Google Scholar 

  • Wang, C., Zhang, Z., Li, B., Wang, H., & Dong, X. (2012). First report of Glomerella leaf spot of apple caused by Glomerella cingulata in China. Plant Disease, 96, 912.

    Article  Google Scholar 

  • Weber, R. W. S., & Palm, G. (2010). Resistance of storage rot fungi Neofabraea perennans, N. alba, Glomerella acutata and Neonectria galligena against thiophanate-methyl in Northern German apple production. Journal of Plant Diseases and Protection, 117, 185–191.

    Article  Google Scholar 

  • Wilkowska, A., & Biziuk, M. (2011). Determination of pesticide residues in food matrices using the QuEChERS methodology. Food Chemistry, 125, 803–812.

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This research was funded by the National Key Basic Research Program of China (2012CB126302), the China Agriculture Research System (CARS-28) and the Taishan Scholar Construction Project of Shandong Province.

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Author notes

  1. Yuran Wang and Pingliang Li contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Integrated Crop Pests Management of Shandong Province, College of Agronomy and Plant Protection, Qingdao Agricultural University, No. 700 Changcheng Road, Qingdao, 266109, Shandong, People’s Republic of China

    Yuran Wang, Pingliang Li, Hongye Zhen & Baohua Li

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  1. Yuran Wang
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  2. Pingliang Li
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  3. Hongye Zhen
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  4. Baohua Li
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Correspondence to Baohua Li.

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Wang, Y., Li, P., Zhen, H. et al. The promotion of thiophanate-methyl and tebuconazole for the continuous control of Glomerella leaf spot in apple leaves by adding pellouxite as a synergistic reagent. J Plant Dis Prot 124, 631–639 (2017). https://doi.org/10.1007/s41348-017-0102-z

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