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Induction of superoxide dismutase, malate dehydrogenase and phenylalanine ammonia-lyase during enhancing resistance of common bean against Xanthomonas axonopodis pv. phaseoli by exogenous salicylic acid

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Abstract

Bacterial blight of common bean caused by Xanthomonas axonopodis pv. phaseoli (Xap) is one of the most devastating diseases and causes serious yield losses worldwide. To extend our understanding for the efficacy of salicylic acid (SA) as a therapeutic agent against bacterial diseases, we have studied the impact of exogenous SA treatment on disease incidence and development during the course of Xap infection. In this study, an enhanced resistance response induced by SA was detected in common bean infected with Xap. On the other hand, SA did not have direct antibacterial effect in vitro. Additionally, we examined the effects of SA on expression of superoxide dismutase (SOD), malate dehydrogenase (MDH) and phenylalanine ammonia-lyase (PAL). SA-pretreated leaves had higher expression of MDH after Xap infection when compared with their counterparts pretreated with water (control). SA/Xap treatments showed more expression of SOD at 24, 48 and 72 h post-pathogen inoculation (hpi) compared to water/Xap samples, whereas PAL expression was significantly different only at 24 hpi. The results showed the contribution of SA as an effective resistance activator and may provide an additional approach in bacterial blight integrated diseases management programs.

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References

  1. Akhavan A, Bahar M, Askarian H, Lak MR, Nazemi A, Zamani Z (2013) Bean common bacterial blight: pathogen epiphytic life and effect of irrigation practices. Springer Plus 2:1–9

    Article  Google Scholar 

  2. Alvarez ME, Pennell RI, Meijer P, Ishikawa A, Dixon RA, Lamb C (1998) Reactive oxygen intermediates mediate a systemic signal network in the establishment of plant immunity. Cell 92:773–784

    Article  CAS  PubMed  Google Scholar 

  3. Chan Z, Wang Q, Xu X, Meng X, Qin G, Li B, Tian S (2008) Functions of defense-related proteins and dehydrogenases in resistance response induced by salicylic acid in sweet cherry fruits at different maturity stages. Proteomics 8:4791–4807

    Article  CAS  PubMed  Google Scholar 

  4. Crampton BG, Hein I, Berger DK (2009) Salicylic acid confers resistance to a biotrophic rust pathogen, Puccinia substriata, in pearl millet (Pennisetum glaucum). Mol Plant Pathol 10:291–304

    Article  CAS  PubMed  Google Scholar 

  5. Czajkowski R, van der Wolf JM, Krolicka A, Ozymko Z, Narajczyk M, Kaczynska N, Lojkowska E (2015) Salicylic acid can reduce infection symptoms caused by Dickeya solani in tissue culture grown potato (Solanum tuberosum L.) plants. Eur J Plant Pathol 141:545–558

    Article  CAS  Google Scholar 

  6. De Gara L, De Pinto MC, Tommasi F (2003) The antioxidant system vis-à-vis reactive oxygen species during plant–pathogen interaction. Plant Physiol Biochem 41:863–870

    Article  Google Scholar 

  7. Dixon RA, Paiva NL (1995) Stress-induced phenylpropanoid metabolism. Plant Cell 7:1085–1097

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Dursun A, Dönmez MF, Sahin F (2002) Identification of resistance to common bacterial blight disease on bean genotypes grown in Turkey. Eur J Plant Pathol 108:811–813

    Article  CAS  Google Scholar 

  9. Eticha D, Zahn M, Bremer M, Yang Z, Rangel A, Rao I, Horst W (2010) Transcriptomic analysis reveals differential gene expression in response to aluminium in common bean (Phaseolus vulgaris) genotypes. Ann Bot 105:1119–1128

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Fu ZQ, Dong X (2013) Systemic acquired resistance: turning local infection into global defense. Annu Rev Plant Biol 64:839–863

    Article  CAS  PubMed  Google Scholar 

  11. Görlach J, Volrath S, Knauf-Beiter G, Hengy G, Beckhove U, Kogel KH, Oostendorp M, Staub T, Ward E, Kessmann H, Ryals J (1996) Benzothiadiazole, a novel class of inducers of systemic acquired resistance, activates gene expression and disease resistance in wheat. Plant Cell 8:629–643

    Article  PubMed  PubMed Central  Google Scholar 

  12. Hayat Q, Hayat S, Irfan M, Ahmad A (2010) Effect of exogenous salicylic acid under changing environment. Environ Exp Bot 68:14–25

    Article  CAS  Google Scholar 

  13. Ishida A, Ookubo K, Ono K (1987) Formation of hydrogen peroxide by NAD(P)H oxidation with isolated cell wall-associated peroxidase from cultured liverwort cells, Marchantia polymorpha L. Plant Cell Physiol 28:723–726

    CAS  Google Scholar 

  14. Kang G, Wang C, Sun G, Wang Z (2003) Salicylic acid changes activities of H2O2-metabolizing enzymes and increases the chilling tolerance of banana seedlings. Environ Exp Bot 50:9–15

    Article  CAS  Google Scholar 

  15. Krantev A, Yordanova R, Janda T, Szalai G, Popova L (2008) Treatment with salicylic acid decreases the effect of cadmium on photosynthesis in maize plants. J Plant Physiol 165:920–931

    Article  CAS  PubMed  Google Scholar 

  16. Lamb C, Dixon RA (1997) The oxidative burst in plant disease resistance. Annu Rev Plant Physiol 48:251–275

    Article  CAS  Google Scholar 

  17. Liang XW, Dron M, Crame CL, Dixon RA, Lamb CJ (1989) Differential regulation of phenylalanine ammonia-lyase genes during plant development and by environmental cues. J Biol Chem 264:14486–14492

    CAS  PubMed  Google Scholar 

  18. Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods 25:402–408

    Article  CAS  PubMed  Google Scholar 

  19. Martinez-Abarca F, Herrera-Cervera JA, Bueno P, Sanjuan J, Bisseling T, Olivares J (1998) Involvement of salicylic acid in the establishment of the Rhizobium meliloti-alfalfa symbiosis. Mol Plant Microbe Interact 11:153–155

    Article  CAS  Google Scholar 

  20. Osdaghi E (2014) Occurrence of common bacterial blight on mungbean (Vigna radiata) in Iran caused by Xanthomonas axonopodis pv. phaseoli. New Dis Rep 30:9

    Article  Google Scholar 

  21. Prithiviraj B, Bais HP, Jha AK, Vivanco JM (2005) Staphylococcus aureus pathogenicity on Arabidopsis thaliana is mediated either by a direct effect of salicylic acid on the pathogen or by SA-dependent, NPR1-independent host responses. Plant J 42:417–432

    Article  CAS  PubMed  Google Scholar 

  22. Prithiviraj B, Bais HP, Weir T, Suresh B, Najarro EH, Dayakar BV, Schweizer HP, Vivanco JM (2005) Down regulation of virulence factors of Pseudomonas aeruginosa by salicylic acid attenuates its virulence on Arabidopsis thaliana and Caenorhabditis elegans. Infect Immun 73:5319–5328

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. Rairdan GJ, Terrence P, Delaney TP (2002) Role of salicylic acid and NIM1/NPR1 in race-specific resistance in Arabidopsis. Genetics 161:803–811

    CAS  PubMed  PubMed Central  Google Scholar 

  24. Reinprecht Y, Yadegari Z, Perry GE, Siddiqua M, Wright LC, McClean PE, Pauls KP (2013) In silico comparison of genomic regions containing genes coding for enzymes and transcription factors for the phenylpropanoid pathway in Phaseolus vulgaris and Glycine max. Front Plant Sci 4:1–25

    Article  Google Scholar 

  25. Singh DP, Moore CA, Gilliland A, Carr JP (2004) Activation of multiple antiviral defense mechanisms by salicylic acid. Mol Plant Pathol 5:57–63

    Article  CAS  PubMed  Google Scholar 

  26. Smith MW, Doolittle RF (1992) A comparison of evolutionary rates of the two major kinds of superoxide dismutases. J Mol Evol 34:175–184

    CAS  PubMed  Google Scholar 

  27. Spoel SH, Dong X (2012) How do plants achieve immunity? Defense without specialized immune cells. Nat Rev Immunol 12:89–100

    Article  CAS  PubMed  Google Scholar 

  28. Subramanian B, Bansal VK, Kav NN (2005) Proteome-level investigation of Brassica carinata-derived resistance to Leptosphaeria maculans. J Agric Food Chem 53:313–324

    Article  CAS  PubMed  Google Scholar 

  29. Talukdar D, Talukdar T (2013) Superoxide-dismutase deficient mutants in common beans (Phaseolus vulgaris L.): genetic control, differential expressions of isozymes, and sensitivity to arsenic. Biomed Res. doi:10.1155/2013/782450

    Google Scholar 

  30. Vlot AC, Dempsey DA, Klessig DF (2009) Salicylic acid, a multifaceted hormone to combat disease. Annu Rev Phytopathol 47:177–206

    Article  CAS  PubMed  Google Scholar 

  31. Wang H, Fan W, Li H, Yang J, Huang J, Zhang P (2013) Functional characterization of dihydroflavonol-4-reductase in anthocyanin biosynthesis of purple sweet potato underlies the direct evidence of anthocyanins function against abiotic stresses. PLoS One 8:e78484. doi:10.1371/journal.pone.0078484

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  32. Walters DR, Fountaine JM (2009) Practical application of induced resistance to plant diseases: an appraisal of effectiveness under field conditions. J Agr Sci 147:523–535

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Plant Protection, College of Agriculture, Shiraz University, Shiraz, Iran

    Ali Safaie Farahani & S. Mohsen Taghavi

  2. Plant Virology Research Center, College of Agriculture, Shiraz University, Shiraz, Iran

    Alireza Afsharifar

Authors
  1. Ali Safaie Farahani
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  2. S. Mohsen Taghavi
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  3. Alireza Afsharifar
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Correspondence to S. Mohsen Taghavi.

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Ali Safaie Farahani, S. Mohsen Taghavi and Alireza Afsharifar declare that they have no conflict of interest.

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Farahani, A.S., Taghavi, S.M. & Afsharifar, A. Induction of superoxide dismutase, malate dehydrogenase and phenylalanine ammonia-lyase during enhancing resistance of common bean against Xanthomonas axonopodis pv. phaseoli by exogenous salicylic acid. J Plant Dis Prot 123, 83–87 (2016). https://doi.org/10.1007/s41348-016-0011-6

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  • DOI: https://doi.org/10.1007/s41348-016-0011-6

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