Synergistic Action of Silver Nanoparticles Synthesized from Silver Resistant Estuarine Pseudomonas aeruginosa Strain SN5 with Antibiotics against Antibiotic Resistant Bacterial Human Pathogens
- 109 Downloads
This study focuses on the extracellular synthesis of silver nanoparticles (AgNPs), carried out using the culture supernatant of silver resistant Pseudomonas aeruginosa strain SN5 isolated from Mandovi estuarine mangrove water sample. AgNPs were characterized using X-Ray diffraction (XRD) analysis which showed high intensity peaks at 28° and 32.5°, characteristic of silver oxide (Ag2O) and confirmed its crystalline nature by referring Joint Committee on Powder Diffraction Standards (JCPDS), File No. 00–076-1393. Transmission electron microscopy (TEM) analysis revealed the nano sized AgNPS particles in the range of 35 nm – 60 nm. AgNPs showed antibacterial activity against both standard cultures of Gram positive and Gram negative bacterial human pathogens. Moreover, the AgNPs also showed antibacterial activity against ampicillin resistant Staphylococccus aureus strain VN3 and ciprofloxacin resistant Vibrio cholera strain VN1 isolated from Mandovi estuary, Goa India, polluted with human feces, domestic and hotel waste. These AgNPs exhibited better antibacterial activity as compared to AgNPs synthesized from plant extract of Honey suckle mistletoe and star anise. Interestringly, synergistic activity was observed when synthesized AgNPs were used in combination with antibiotics ampicillin and ciprofloxacin against ampicillin resistant Staphylococccus aureus strain VN3 and ciprofloxacin resistant Vibrio cholera strain VN1. Thus these AgNPs can be employed in cosmetics and wound dressings as a nanoweapon to control human bacterial pathogens.
KeywordsEstuarine Pseudomonas aeruginosa Silver nanoparticles Pathogens Nanoweapon Synergistic activity
The authors would like to thank IIT Powai for TEM analysis. Also we appreciate help from Sonica Chari, Annelia Enchimani and Kalpita Vast, Goa University. Dr. Milind Mohan Naik thank SERB-DST for young scientist project (File Number: YSS/2014/000258).
Compliance with Ethical Standards
Conflict of Interest
On behalf of all authors, the corresponding author states that there is no conflict of interest.
- Arunkumar M, Narayanan M, Balakumar S, Ramalingam S, Priyadharshni (2013) Antiquorum sensing and antibacterial activity of silver nanoparticles synthesized by mutant Klebsiella pneumoniae MTCC 3354. Asian J Chem 25.17: 9961–9964Google Scholar
- Ashley NB, Kathryn S, Tova S, Jiangrui L, Sherine O, Maria S (2012) Nanoparticles functionalized with ampicillin destroy multiple- antibiotic-resistant isolates of Pseudomonas aeruginosa and Enterobacter aerogenes and methicillin-resistant Staphylococcus aureus. Appl Microbiol Biotechnol 78:2768–2774Google Scholar
- Chari SJ, Naik MN (2015) Green synthesis of silver nanoparticles from the spice star Anise (Illicium verum) and evaluation of its antimicrobial activity. Goa University, DissertationGoogle Scholar
- Dhoondia ZH, Chakraborty H (2012) Lactobacillus mediated synthesis of silver oxide nanoparticles. Nanomater nanotechnol 2(15):1–7Google Scholar
- Enchimani A, Naik M (2015) Biogenic synthesis of silver nanoparticles using mango (Mangifera indica) parasitic plant Honey suckle mistletoe (Loranthus falcutus) and evaluation of it’s antimicrobial activity. Goa University, DissertationGoogle Scholar
- Huijing B, Xiaoxu Y, Chen X, Xue L, Zhaoyang L, Dianjun W, Yunde L (2015) New toxicity mechanism of silver nanoparticles: promoting apoptosis and inhibiting proliferation. PLoS One 10(3):0122535Google Scholar
- Kumar CSR, Joseph MM, Kumar TRG, Renjith KR, Manju MN, Chandramohanakumar N (2010) Spatial variability and contamination of heavy metals in the inter-tidal systems of a tropical environment. Int J Environ Res Publ Health 4(4):691–700Google Scholar
- Nikolaos P, Louise EH (2014) Biological synthesis of metallic nanoparticles by bacteria, fungi and plants. J Nanomed Nanotechnol 5:233Google Scholar
- Odonkor ST, Addo KK (2011) Bacteria resistance to antibiotics: recent trends and challenges. Int J Biol Med Res 2(4):1204–1210Google Scholar
- Sneath PHA, Mair NS, Sharpe ME, Holt JG (1986) Bergey’s Manual of systematic Bacteriology. Williams & Wilkins, BaltimoreGoogle Scholar
- Vast KS, Naik MM (2016) Antibacterial activity of novel synthetic thiazolidine derivatives against multidrug resistant pathogenic bacteria from Mandovi estuary and standard ATCC bacterial cultures. Goa University, DissertationGoogle Scholar
- Woo KJ, Hye C, Ki K, Sook S, So K, Yong P (2008) Antibacterial activity and mechanism of action of the silver ion in Staphylococcus aureus and Escherichia coli. Appl Microbiol Biotechnol 74(7):2171–2178Google Scholar