Abstract
The increased awareness regarding the economic and environmental impact of olive oil production waste has motivated a notable interest in reusing byproducts within the industry. By mitigating ecological risks associated with olive mill waste, this research addresses concerns about waste disposal and contributes to sustainable practices within the olive oil industry. This study focuses on extracting and characterizing two key biopolymers, cellulose and lignin, obtained from locally sourced two-phase olive mill waste. The development of a straightforward yet efficient extraction protocol yielded promising results, with 11.72% cellulose and 12.5% lignin successfully isolated from the waste material. The extracted biopolymers underwent thorough characterization utilizing advanced analytical techniques, such as Fourier-transformed spectroscopy, thermogravimetric analysis (TGA/DTG), X-ray diffraction (XRD), and scanning electron microscopy (SEM). These methods provided comprehensive insights into the structural and thermal properties of cellulose and lignin, confirming the success of the extraction process. Furthermore, the isolated biopolymers hold significant potential for diverse applications, further expanding the possibilities for their utilization. This comprehensive approach emphasizes the importance of repurposing byproducts to enhance economic and environmental sustainability in olive oil production.
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The authors declare that the data supporting the findings of this study are available within the paper. The dataset used and/or analyzed during the current study is available from the authors on reasonable request.
References
Arasaretnam S, Venujah K (2019) Preparation of soaps by using different oils and analyze their properties. Nat Prod Chem Res 7(1):1–4. https://doi.org/10.4172/2329-6836.1000357
Arenas-Castro S, Gonçalves JF, Moreno M, Villar R (2020) Projected climate changes are expected to decrease the suitability and production of olive varieties in southern Spain. Sci Total Environ. https://doi.org/10.1016/j.scitotenv.2019.136161
Ayouch I, Kassem I, Kassab Z, Barrak I, Barhoun A, Jacquemin J, Draoui K, El Achaby M (2021) Crosslinked carboxymethyl cellulose-hydroxyethyl cellulose hydrogel films for adsorption of cadmium and methylene blue from aqueous solutions. Surf Interfaces. https://doi.org/10.1016/j.surfin.2021.101124
Azaryouh L, Abara H, Kassab Z, Ablouh EH, Aboulkas A, El Achaby M, Draoui K (2023) Hybrid carbonaceous adsorbents based on clay and cellulose for cadmium recovery from aqueous solution. RSC Adv 13(10):6954–6965. https://doi.org/10.1039/d2ra08287j
Azzaz AA, Ghimbeu CM, Jellai S, El-Bassi L, Jeguirim M (2022) Olive mill by-products thermochemical conversion via hydrothermal carbonization and slow pyrolysis: detailed comparison between the generated hydrochars and biochars characteristics. Processes. https://doi.org/10.3390/pr10020231
Başakçılardan Kabakcı S, Baran SS (2019) Hydrothermal carbonization of various lignocellulosics: fuel characteristics of hydrochars and surface characteristics of activated hydrochars. Waste Manag 100:259–268. https://doi.org/10.1016/j.wasman.2019.09.021
Bouhia Y, Hafidi M, Ouhdouch Y, Lyamlouli K (2023) Olive mill waste sludge: from permanent pollution to a highly beneficial organic biofertilizer: a critical review and future perspectives. Ecotoxicol Environ Saf 259:114997. https://doi.org/10.1016/j.ecoenv.2023.114997
Chauhan K, Kumar A, Goswami K, Negi L, Chauhan A, Madan K, Jain S (2023) Lignin extraction from lignocellulosic biomass (sugarcane bagasse) and its potential application as a feedstock for fuel production. Mater Today Proc 78:688–694. https://doi.org/10.1016/j.matpr.2022.12.190
Dash S, Bhavanam A, Gera P (2023) Parametric optimization of kraft pulping of wheat straw for extraction of lignin using response surface methodology. Biomass Convers Biorefin. https://doi.org/10.1007/s13399-023-04011-4
El Achaby M, Kassab Z, Barakat A, Aboulkas A (2018) Alfa fibers as viable sustainable source for cellulose nanocrystals extraction: application for improving the tensile properties of biopolymer nanocomposite films. Ind Crops Prod 112:499–510. https://doi.org/10.1016/j.indcrop.2017.12.049
El Bouchtaoui FZ, Ablouh EH, Kassem I, Kassab Z, Sehaqui H, El Achaby M (2022a) Slow-release fertilizers based on lignin–sodium alginate biopolymeric blend for sustained N-P nutrients release. J Coat Technol Res 19(5):1551–1565. https://doi.org/10.1007/s11998-022-00629-7
El Bouchtaoui FZ, Ablouh EH, Mhada M, Kassem I, Salim MH, Mouhib S, Kassab Z, Sehaqui H, El Achaby M (2022b) Methylcellulose/lignin biocomposite as an eco-friendly and multifunctional coating material for slow-release fertilizers: effect on nutrients management and wheat growth. Int J Biol Macromol 221(June):398–415. https://doi.org/10.1016/j.ijbiomac.2022.08.194
Erses Yay AS, Birinci B, Açıkalın S, Yay K (2021a) Hydrothermal carbonization of olive pomace and determining the environmental impacts of post-process products. J Clean Prod 315(June):128087. https://doi.org/10.1016/j.jclepro.2021.128087
Erses Yay AS, Birinci B, Açıkalın S, Yay K (2021b) Hydrothermal carbonization of olive pomace and determining the environmental impacts of post-process products. J Clean Prod. https://doi.org/10.1016/j.jclepro.2021.128087
García R, Pizarro C, Lavín AG, Bueno JL (2012) Characterization of Spanish biomass wastes for energy use. Biores Technol 103(1):249–258. https://doi.org/10.1016/j.biortech.2011.10.004
Hassan ML, Abou-Zeid RE, Fadel SM, El-Sakhawy M, Khiari R (2014) Cellulose nanocrystals and carboxymethyl cellulose from olive stones and their use to improve paper sheets properties. Int J Nanopart 7(3–4):261–277. https://doi.org/10.1504/ijnp.2014.067613
Islam MA, Limon MSH, Romić M, Islam MA (2021) Hydrochar-based soil amendments for agriculture: a review of recent progress. Arab J Geosci. https://doi.org/10.1007/s12517-020-06358-8
Karagoz P, Khiawjan S, Marques MPC, Santzouk S, Bugg TDH, Lye GJ (2023) Pharmaceutical applications of lignin-derived chemicals and lignin-based materials: linking lignin source and processing with clinical indication. Biomass Convers Biorefin. https://doi.org/10.1007/s13399-023-03745-5
Katimbo A, Kiggundu N, Kizito S, Kivumbi HB, Tumutegyereize P (2014) Potential of densification of mango waste and effect of binders on produced briquettes. Agric Eng Int CIGR J 16(4):146–155
Kerr WA (2021) Agriculture after a year with COVID-19: any long-term implications for international trade policy? Can J Agric Econ 69(2):261–267. https://doi.org/10.1111/cjag.12274
Lappi H, Alén R (2011) Pyrolysis of vegetable oil soaps—palm, olive, rapeseed and castor oils. J Anal Appl Pyrol 91(1):154–158. https://doi.org/10.1016/j.jaap.2011.02.003
Mennani M, Kasbaji M, Ait Benhamou A, Boussetta A, Mekkaoui AA, Grimi N, Moubarik A (2023) Current approaches, emerging developments and functional prospects for lignin-based catalysts—a review. Green Chem 25(8):2896–2929. https://doi.org/10.1039/d3gc00072a
Mennani M, Kasbaji M, Ait A, Ablouh E (2024a) Chemosphere Lignin-functionalized cobalt for catalytic reductive degradation of organic dyes in simple and hybrid binary systems. Chemosphere 350(December 2023):141098. https://doi.org/10.1016/j.chemosphere.2023.141098
Mennani M, Kasbaji M, Ait Benhamou A, Boussetta A, Kassab Z, El Achaby M, Grimi N, Moubarik A (2024b) The potential of lignin-functionalized metal catalysts—a systematic review. Renew Sustain Energy Rev 189(1):113936. https://doi.org/10.1016/j.rser.2023.113936
Muñoz JC, Grifoll V, Pérez-Clavijo M, Saiz-Santos M, Lizundia E (2023) Techno-economic assessment of chitin nanofibrils isolated from fungi for a pilot-scale biorefinery. ACS Sustain Resour Manag. https://doi.org/10.1021/acssusresmgt.3c00030
Nait M’Barek H, Arif S, Taidi B, Hajjaj H (2020) Consolidated bioethanol production from olive mill waste: wood-decay fungi from central Morocco as promising decomposition and fermentation biocatalysts. Biotechnol Rep. https://doi.org/10.1016/j.btre.2020.e00541
Okegbile OJ, Hassan AB, Mohammed A, Irekeola BJ (2014) Effect of starch and gum arabic binders in the combustion characteristics of briquette prepared from sawdust. Int J Sci Eng Res 5(3):1005–1009
Pavez IC, Lozano-Sánchez J, Borrás-Linares I, Nuñez H, Robert P, Segura-Carretero A (2019) Obtaining an extract rich in phenolic compounds from olive pomace by pressurized liquid extraction. Molecules 24(17):1–17. https://doi.org/10.3390/molecules24173108
Rapheal IA, Moki EC, Hassan LG, Abimbola AI (2018) Effect of binder on the physico-chemical properties of fuel briquettes produced from sugarcane peels at ambient temperature and die pressure. AASCIT J Energy 5(September):28–31
Rizzi V, D’Agostino F, Fini P, Semeraro P, Cosma P (2017) An interesting environmental friendly cleanup: the excellent potential of olive pomace for disperse blue adsorption/desorption from wastewater. Dyes Pigm 140:480–490. https://doi.org/10.1016/j.dyepig.2017.01.069
Sánchez-Sánchez C, González-González A, Cuadros-Salcedo F, Cuadros-Blázquez F (2020) Two-phase Olive mill waste: a circular economy solution to an imminent problem in Southern Europe. J Clean Prod. https://doi.org/10.1016/j.jclepro.2020.122789
Volpe M, Wüst D, Merzari F, Lucian M, Andreottola G, Kruse A, Fiori L (2018) One stage olive mill waste streams valorisation via hydrothermal carbonisation. Waste Manag 80:224–234. https://doi.org/10.1016/j.wasman.2018.09.021
Volpe M, Messineo A, Mäkelä M, Barr MR, Volpe R, Corrado C, Fiori L (2020) Reactivity of cellulose during hydrothermal carbonization of lignocellulosic biomass. Fuel Process Technol 206(April):106456. https://doi.org/10.1016/j.fuproc.2020.106456
Zhou F, Li K, Hang F, Zhang Z, Chen P, Wei L, Xie C (2022) Efficient removal of methylene blue by activated hydrochar prepared by hydrothermal carbonization and NaOH activation of sugarcane bagasse and phosphoric acid. RSC Adv 12(3):1885–1896. https://doi.org/10.1039/d1ra08325b
Acknowledgements
This work is supported by the Moroccan Ministry of Higher Education, Scientific Research and Innovation and the OCP Foundation, which funded this work through the APRD research program. The authors express their gratitude to Dr. Mehdi Mennani for his invaluable contribution in improving the quality of this paper and for his assistance in preparing the economic review, the authors also are grateful to Professor Jones Alami, head of Materials Science and Nanoengineering Department (MSN) at Mohammed VI Polytechnic University (UM6P) Ben guerir for supporting this work at MSN.
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L.A.: conceptualization, methodology, investigation, formal analysis, writing—original draft, and writing—review and editing. Z.K.: supervision, conceptualization, methodology, validation, and review and editing. M.J.: methodology and formal analysis. A.M.: methodology and formal analysis. K.E.H.: supervision and review and editing. M.E.A.: supervision, conceptualization, methodology, resources, validation and review and editing. A.A.: supervision, conceptualization, resources, and review and editing.
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Communicated by Walid Elfalleh.
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Azaryouh, L., Kassab, Z., Jaouahar, M. et al. Integral valorization of two-phase olive mill waste: extraction of cellulose and lignin and their characterization. Euro-Mediterr J Environ Integr (2024). https://doi.org/10.1007/s41207-024-00521-3
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DOI: https://doi.org/10.1007/s41207-024-00521-3