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Chemical synthesis of chitosan (CS)–sodium alginate (ALG) nanoparticles

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Alginate nanoparticles (CS/ALG) were synthesised via ionic-gelation of an alginate core, followed by polyelectrolyte-complexation of chitosan polymers. AG was diluted with distilled water before CaCl2 was added to enhance overall viscosity of the solution (solution 1). A 1% acetic acid solution was also used to dissolve CS (solution 2). Analysis of nanoparticles' morphology and structure was carried out using transmission electron microscopy as well as Fourier transform infrared spectroscopy (FTIR). The AG/CS NPs had distinct UV absorption spectra with a wavelength of 325 nm, which revealed the basic bandgap absorption of AG/CS nanocrystals XRD findings reveal that AG/CS-based samples had a single phase hexagonal wurtzite structure with no impurities FT-IR spectrum characteristics of AG/CS NPs indicate that the bonded N–H/C-H peaks may be assigned to 3442.39 cm−1. SEM photos in clearly showed the existence of spherical-shaped nanoparticles. AG/CS NPs ranged in size from 10 to 50 nm, with an average crystallite size of 15 nm.

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  1. Mirzaei M, Darroudi M (2017) Zinc oxide nanoparticles: biological synthesis and biomedical applications. Ceram Int 43:907–914

    Article  Google Scholar 

  2. Agarwal H, Kumar SV, Rajeshkumar S (2017) A review on green synthesis of zinc oxide nanoparticles–an eco-friendly approach. Resource Efficient Technol 3:406–413

    Article  Google Scholar 

  3. Grieshaber D, MacKenzie R, Voros J, Reimhult E (2008) Electrochemical biosensors-sensor principles and architectures. Sensors 8:1400–1458

    Article  Google Scholar 

  4. Zeng L, Qin C, Wang W, Chi W, Li W (2008) Absorption and distribution of chitosan in mice after oral administration. Carbohyd Polym 71(3):435–440

    Article  Google Scholar 

  5. De S, Robinson D (2003) ‘Polymer relationships during preparation of chitosan- alginate and poly-L-lysine-alginate nanospheres. J Control Rel 89:101–112

    Article  Google Scholar 

  6. Sarmento B, Ribeiro AJ, Veiga F, Ferreira DC et al (2007) Insulin-loaded nanoparticles are prepared by alginate ionotropic pre-gelation followed by chitosan polyelectrolyte complexation. J Nanosci Nanotechnol 7:2833–2841

    Article  Google Scholar 

  7. Florence A (1997) The oral absorption of micro- and nanoparticulates: Neither exceptional nor unusual. Pharm Res 14:259–266

    Article  Google Scholar 

  8. Murata Y, Jinno D, Liu D, Isobe T et al (2007) The drug release profile from calcium-induced alginate gel beads coated with an alginate hydrolysate. Molecules 12(11):2559–2566

    Article  Google Scholar 

  9. Shi J, Alves NM, Mano JF (2008) Chitosan coated alginate beads containing poly(N-isopropylacrylamide) for dual-stimuli-responsive drug release. J Biomed Mater Res B Appl Biomater 84(2):595–603

    Article  Google Scholar 

  10. Smidsrod O, Skjakbraek G (1990) Alginate as immobilization matrix for Cells. Trends Biotechnol 8:71–78

    Article  Google Scholar 

  11. Gombotz WR, Wee SF (1998) Protein release from alginate matrices. Adv Drug Deliv Rev 31:267–285

    Article  Google Scholar 

  12. George M, Abraham TE (2006) Polyionic hydrocolloids for the intestinal delivery of protein drugs: alginate and chitosan -a review. J Control Release 114(1):1–14

    Article  Google Scholar 

  13. Yang J, Xie YJ, He W (2011) Research progress on chemical modification of alginate: a review. Carbohyd Polym 84(1):33–39

    Article  Google Scholar 

  14. Nair LS, Laurencin CT (2007) Biodegradable polymers as biomaterials. Prog Polym Sci 32:762–798

    Article  Google Scholar 

  15. Pan Y, Li YJ, Zhao HY, Zheng JM et al (2002) Bioadhesive polysaccharide in protein delivery system: chitosan nanoparticles improve the intestinal absorption of insulin in vivo. Int J Pharm 249:139–147

    Article  Google Scholar 

  16. Lee KY, Mooney DJ (2012) Alginate: properties and biomedical applications. Prog Polym Sci 37(1):106–126

    Article  Google Scholar 

  17. Mladenovska K, Cruaud O, Richomme P, Belamie E et al (2007) 5-ASA loaded chitosan-Ca-alginate microparticles: Preparation and physicochemical characterization. Int J Pharm 345:59–69

    Article  Google Scholar 

  18. Chandy T, Sharma CP (1990) Chitosan-as a biomaterial. Biomater Artif Cells Artif Organs 18:1–24

    Article  Google Scholar 

  19. Venkatesan J, Kim SK (2010) Chitosan composites for bone tissue engineering-an overview. Mar Drugs 8:2252–2266

    Article  Google Scholar 

  20. Kumar S, Dutta J, Dutta PK (2009) Preparation and characterization of N-heterocyclic chitosan derivatives based gels for biomedical applications. Int J Biol Macromol 45:330–337

    Article  Google Scholar 

  21. Karagozlu MZ, Kim SK. (2014) Anticancer effects of chitin and chitosan derivatives’. Chapter Twelve, In Advances in Food and Nutrition Research; Kim, S.K., Ed.; Academic Press: Waltham, MA, USA, 2014; Volume 72, pp 215–225

  22. Martins AF, Facchi SP, Follmann HD, Pereira AG, Rubira AF, Muniz EC (2014) Antimicrobial activity of chitosan derivatives containing N-quaternized moieties in its backbone: a review. Int J Mol Sci 15:20800–20832

    Article  Google Scholar 

  23. Ngo DH, Kim SK. 2014. Chapter Two—Antioxidant effects of chitin, chitosan, and their derivatives. In Advances in Food and Nutrition Research; Kim, S.K., Ed.; Academic Press: Waltham, MA, USA, 2014; Volume 73, pp. 15–31

  24. Aranaz I, Mengíbar M, Harris R, Panos I, Miralles B, Acosta N, Galed G, Heras A (2009) Functional characterization of chitin and chitosan. Curr Chem Biol 3:203–230

    Google Scholar 

  25. Onsosyen E, Skaugrud O (1990) Metal recovery using chitosan. J Chem Technol Biotechnol 49:395–404

    Article  Google Scholar 

  26. Felt O, Buri P, Gurny R (1998) ‘Chitosan: a unique polysaccharide for drug delivery’, Drug Dev. Ind Pharm 24:979–993

    Article  Google Scholar 

  27. Han LK, Kimura Y, Okuda H (1999) Reduction in fat storage during chitin-chitosan treatment in mice fed a high-fat diet. Int J Obes Relat Metab Disord 23:174–179

    Article  Google Scholar 

  28. Zhang Y, Zhang M (2002) Three-dimensional macroporous calcium phosphate bioceramics with nested chitosan sponges for load-bearing bone implants. J Biomed Mater Res 61:1–8

    Article  Google Scholar 

  29. Uthaman S, Lee SJ, Cherukula K, Cho C, Park IK (2015) Polysaccharide-coated magnetic nanoparticles for imaging and gene therapy, Bio Med Res Int 959175.

  30. De Jong WH, Borm PJ (2008) Drug delivery and nanoparticles: Applications and hazards. Int J Nanomed 3:133–149

    Article  Google Scholar 

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Correspondence to Deepa Bhagat.

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Srujana, S., Bhagat, D. Chemical synthesis of chitosan (CS)–sodium alginate (ALG) nanoparticles. Nanotechnol. Environ. Eng. 7, 289–296 (2022).

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