The effect of multi-frequency whole-body vibration on night-shifted mouse model
The circadian rhythm controls several biological activities; therefore, a disorganized circadian rhythm may cause fatal health problems. The aim of this study was to assess the effects of circadian rhythm disturbances induced by simulated night shift activities on the abdominal adipose tissue, bone microstructures and muscle volume in the tibiae of mice. Moreover, we evaluated the effects of multi-frequency whole-body vibration as a countermeasure against the consequences of circadian rhythm disturbances. Twenty-four 5-week-old C57BL/6J male mice were equally assigned to three groups: the normal group (Nor), night shift group (NS), and night shift with multi-frequency whole-body vibration group (NS + V). The NS and NS + V groups were exposed to circadian rhythm disturbances for 4 weeks with 3-day intervals by changing the day and night cycle based on 7 o’clock. After 4 weeks, morphological changes in the adipose tissue, bone microstructures and muscle volume in the tibiae were evaluated from three-dimensional images using in vivo micro-computed tomography. As a result, the volume of the abdominal adipose tissue was significantly higher in the NS than in the Nor and NS + V groups. Also, the microstructures of the tibia were more enhanced in the NS + V than the NS group. The volume of tibial muscle was increased in all groups, while there were no significant changes in muscle volume. From these results, we can conclude that circadian rhythm disturbances induced by night shift activities may reduce bone condition and increase the accumulation of abdominal adipose tissue and these negative effects may be prevented or improved through applying multi-frequency whole-body vibration.
KeywordsCircadian rhythm Whole-body vibration Micro-CT Adipose tissue Muscle Trabecular bone
This work was supported by the Yonsei University Future-leading Research Initiative of 2015 (2017-22-0143).
Compliance with ethical standards
Ethical Committee Permission and Animals/the protocols for all procedures were approved by the Yonsei University Animal Care Committee (YWC-130729-1) and twenty-four 5-week-old male C57BL/6 mice (19 ± 1.2 g) were used in this study.
Conflict of interest
All of the authors have nothing to disclose.
- 3.Koo KS. The effects of sleep deprivation on the changes of eeg, fatigue metabolic substrate, and stress hormone following maximal exercise. Korean J Growth Dev. 2010;18(1):57–64.Google Scholar
- 4.Wang JH, et al. The association between shift work and bone mineral density: analysis of 2008–2009 Korean National Health and Nutrition Examination Survey. Korean J Occup Environ Med. 2012;24(3):274–86.Google Scholar
- 5.Hwang E-H, Kang J-S. A study on job involvement according to working pattern and daytime sleepiness among hospital nurses. J East West Nurs Res. 2011;17(2):81–6.Google Scholar
- 15.Andrews JL, Zhang X, McCarthy JJ, McDearmon EL, Hornberger TA, Russell B, Campbell KS, Arbogast S, Reid MB, Walker JR, Hogenesch JB, Takahashi JS, Essera KA. CLOCK and BMAL1 regulate MyoD and are necessary for maintenance of skeletal muscle phenotype and function. Proc Natl Acad Sci. 2010;107(44):19090–5.CrossRefPubMedPubMedCentralGoogle Scholar
- 26.Hwang D, Kim S, Lee H, Lee S, Seo D, Cho S, Chen S, Han T, Kim HS. The effects of whole body vibration in the aspect of reducing abdominal adipose tissue in high-fat diet mice model. J Biomed Eng Res. 2017;38(1):49–55.Google Scholar
- 33.Ko C-Y, Seo DH, Kim HS. Deterioration of bone quality in the tibia and fibula in growing mice during skeletal unloading: gender-related differences. J Biomech Eng. 2011;133(11).Google Scholar
- 40.Verschueren SM, Roelants M, Delecluse C, Swinnen S, Vanderschueren D, Boonen S. Effect of 6-month whole body vibration training on hip density, muscle strength, and postural control in postmenopausal women: a randomized controlled pilot study. J Bone Miner Res. 2004;19(3):352–9.CrossRefPubMedGoogle Scholar