Abstract
Bacterial cellulose (BC) is an extremely pure and highly valuable biomaterial. However, its production cost poses a challenge for large-scale manufacturing. This study explores a cost-effective approach by co-cultivating lactic acid bacteria with cellulose-synthesizing bacteria. Four BC-producing isolates from spoiled fruits and four lactic acid bacteria from fermented buttermilk were isolated and characterized. Growth studies demonstrated successful lactic acid bacteria cultivation in HS media. Co-cultivation of cellulose-synthesizing bacteria and lactic acid bacteria showed enhanced BC production, with a twofold increase in dry weight (0.35 g/150 ml) compared to the monoculture of cellulose-synthesizing bacteria (0.15 g/150 ml). Scanning electron microscopy revealed improved BC microfibril quality in co-culture. Reversed-phase HPLC confirmed higher lactic acid concentrations in co-culture. 16S rRNA sequence analysis revealed that lactic acid bacteria had a 100% match with Lactococcus lactis. These findings highlight the potential of co-cultivation for cost-effective BC production and lactic acid yield, offering a sustainable approach to biomaterial production.
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Abbreviations
- BC:
-
Bacterial cellulose
- MRS:
-
DeMan, Rogosa and Sharpe
- HS:
-
Hestrin and Schramm
- LAB:
-
Lactic acid bacteria
- CSB:
-
Cellulose-synthesizing bacteria
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The authors would like to express their gratitude to the PG Department of Biotechnology at Alva’s College for providing all essential materials and equipment for carrying out this work.
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Akki, A.J., Hiremath, L.D. & Badkillaya, R.R. Harnessing Symbiotic Association of Lactic Acid Bacteria and Cellulose-Synthesizing Bacteria for Enhanced Biological Activity. Iran J Sci 48, 311–320 (2024). https://doi.org/10.1007/s40995-023-01567-8
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DOI: https://doi.org/10.1007/s40995-023-01567-8