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An Epidemiological Correlation of Oral Candidiasis Mice Model Study of an Isolate from Mollugo pentaphylla Linn. and In silico Docking Approach

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Abstract

The aim of the present study was to isolate the active compound against Candida albicans from the methanolic extract of aerial part of Mollugo pentaphylla Linn. and to peruse in vivo animal model of oral candidiasis. The study was designed to quantify the oral candida infection with different statistical approach and histopathological observation of infected tongue on treatment with 100 µg/mL of the isolated compound. The significance of age, body weight and tongue weight was also correlated with disease treatment on either sex of animal. The isolated active compound was characterized as stigmasterol (EX-1) based on FTIR, DSC, 1H NMR, 13C NMR and mass spectral analysis. An in silico docking approach predicted strong negative binding energy efficacy of stigmasterol with C. albicans PKh Kinase protein unveiled an in-visual perception for anticandidal efficacy.

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Acknowledgements

The authors are thankful to the DST, Government of India, for providing the fund under the INSPIRE Fellowship toward Mr. Partha Niyogi (IF 130395); Siksha ‘O’ Anusandhan Deemed to be University; and IMS & SUM Hospital, Odisha, India, to carry out the research work. The authors are also thankful to taxonomist, Dr. A. K. Pradhan, Professor, Department of Botany, PPD Mahavidyalaya, Tigiria, Cuttack, Orissa, India, to authenticate the plant.

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Authors and Affiliations

  1. School of Pharmaceutical Sciences, Siksha ‘O’ Anusandhan Deemed to be University, Kalinga Nagar, Ghatikia, Bhubaneswar, Odisha, 751003, India

    Partha Niyogi, Snigdha Pattnaik, Laxmidhar Maharana & Rajaram Mohapatra

  2. International Institute of Information Technology, Pune, Maharastra, 411057, India

    Swati P. Kolet

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  1. Partha Niyogi
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  2. Snigdha Pattnaik
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  3. Laxmidhar Maharana
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  4. Rajaram Mohapatra
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Correspondence to Snigdha Pattnaik.

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Niyogi, P., Pattnaik, S., Maharana, L. et al. An Epidemiological Correlation of Oral Candidiasis Mice Model Study of an Isolate from Mollugo pentaphylla Linn. and In silico Docking Approach. Iran J Sci Technol Trans Sci 43, 785–799 (2019). https://doi.org/10.1007/s40995-018-0619-4

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  • DOI: https://doi.org/10.1007/s40995-018-0619-4

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