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Purification and Lignocellulolytic Potential of Cellulase from Newly Isolated Acinetobacter indicus KTCV2 Strain

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Abstract

A novel approach of cellulase-based co-culture producing bioethanol using low-cost nutrient medium has been employed for the study, and docking strategies provided the information of cellobiose and cellotetraose inhibitors during cellulase production. The Acinetobacter species was isolated from termite gut and confirmed in 16s rDNA analysis. SDS-PAGE reveals the molecular weight of purified cellulose was 45 kDa. Cellulose activity was achieved maximum of 1.2 IU/mL at 48th hour of incubation, and about 80% of polysaccharides were converted into simple sugars. The enzyme activity was optimum at different physiological conditions like temperature at 37 °C, pH 7.0 and with 4% concentration of banana peduncle extract powder. Upon reaching maximum cellulolytic activity of 0.594 IU/mL, the percentage of ethanol produced from cellulosic hydrolysate using S. cerevisiae reached maximum ethanol (18.3 g/L) during 48 h of incubation. Cellulase produced by Acinetobacter indicus KTCV2 strain exhibits a short incubation period (48 h) and produces cellulase in broader pH and temperature ranges. Banana peduncle offers a cheapest raw material composed of cellulosic biomass in agricultural practices, which served as a good substrate for the production of ethanol.

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Acknowledgements

The authors would like to acknowledge the support of Vignan’s Foundation for Science Technology and Research (Deemed to be University), Guntur, India.

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Authors and Affiliations

  1. Department of Biotechnology, Vignan’s Foundation for Science Technology and Research, Vadlamudi, Guntur, Andhra Pradesh, 522213, India

    Abraham Peele Karlapudi, T. C. Venkateswarulu, Krupanidhi Srirama, Vijaya Ramu Dirisala, Bala Pratyusha Kamarajugadda & Rohini Krishna Kota

  2. Department of Biotechnology, Vikrama Simhapuri University, Nellore, 524320, India

    Vidya Prabhakar Kodali

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  1. Abraham Peele Karlapudi
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  2. T. C. Venkateswarulu
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  3. Krupanidhi Srirama
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  4. Vijaya Ramu Dirisala
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  5. Bala Pratyusha Kamarajugadda
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  6. Rohini Krishna Kota
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  7. Vidya Prabhakar Kodali
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Correspondence to Abraham Peele Karlapudi.

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Karlapudi, A.P., Venkateswarulu, T.C., Srirama, K. et al. Purification and Lignocellulolytic Potential of Cellulase from Newly Isolated Acinetobacter indicus KTCV2 Strain. Iran J Sci Technol Trans Sci 43, 755–761 (2019). https://doi.org/10.1007/s40995-018-0600-2

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  • DOI: https://doi.org/10.1007/s40995-018-0600-2

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