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Moricandia arvensis Chloroform Root Extract Reduces Calpain Activity, ROS Production in Colorectal Cancer Cells and Melanin Biosynthesis

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Iranian Journal of Science and Technology, Transactions A: Science Aims and scope Submit manuscript

Abstract

Medicinal plants have been used for centuries to fight against all types of diseases, essentially certain types of cancer. In this study, we investigate the potential of Moricandia arvensis chloroform root extract (ChlR) as anticancer agent by evaluating its effect on viable cell number, oxidation, the sensitivity of cancer cells to apoptosis and melanin biosynthesis. ChlR extract decreased the proliferation of Caco-2 and BE cell lines by 99.60 and 95.21%, respectively. Furthermore, the extract exhibited significant inhibitory activity (99.43%) against calpain after 2 h of incubation with Caco-2 cells, suggesting its possible use as an anticancer agent. ChlR also enhanced significant antioxidant activity (IP = 100%) after 2 h of incubation with the two colorectal cancer cell lines and a significant inhibition of melanin synthesis. Targeting calpain using ChlR extract could be an interesting way to prevent and/or treat colorectal cancers; in addition, this extract might be used as a skin whitening agent.

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Acknowledgements

We acknowledge Campus France and Ministry of Higher Education, Scientific Research and Technology, Tunisia for the financial support of this study (Action Intégrée de Coopération Inter universitaire Franco-Tunisienne).

Funding

Funding was provided by Campus France (EGIDE; 784339J).

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Authors and Affiliations

  1. Laboratory of Molecular and Cellular Biology, Faculty of Dental Medicine, Monastir, Tunisia

    Ines Skandrani & Leila Chekir

  2. Unit of Pharmacognosy/Molecular Biology 99/UR/07-03, Faculty of Pharmacy, Monastir, Tunisia

    Kamel Ghedira & Leila Chekir

  3. INSERM U911 (CRO2), University of Aix-Marseille, 27 boulevard Jean Moulin, 13385, Marseille, France

    Ludovic Leloup & Hervé Kovacic

Authors
  1. Ines Skandrani
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  2. Ludovic Leloup
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  3. Hervé Kovacic
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  4. Kamel Ghedira
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  5. Leila Chekir
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Correspondence to Ines Skandrani.

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Skandrani, I., Leloup, L., Kovacic, H. et al. Moricandia arvensis Chloroform Root Extract Reduces Calpain Activity, ROS Production in Colorectal Cancer Cells and Melanin Biosynthesis. Iran J Sci Technol Trans Sci 43, 747–753 (2019). https://doi.org/10.1007/s40995-018-0597-6

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  • DOI: https://doi.org/10.1007/s40995-018-0597-6

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