Abstract
Being a part of Chinese medicine and western herbalism, Bidens biternata is highly medicinal, used to cure almost 26 diseases including some dreadful skin and lung infections. The present study was conducted to evaluate phytochemical, antimicrobial, and antioxidant activities of extracts obtained from B. biternata. The whole plant was powdered, extracted with methanol and chloroform and subjected to qualitative and quantitative phytochemical screening. Extracts were subjected to antibacterial activities through agar well and paper disc diffusion method as well as antifungal activities through agar tube dilution method. Antioxidant analysis was done by DPPH, reducing power and phosphomolybdenum assay. Phytochemical analysis indicated the presence of alkaloids (1.13 mg g−1), flavanoids (0.39 mg g−1), phenols (0.17 mg g−1) and tannins (1.32 mg g−1). Both plant extract showed substantial antimicrobial and antioxidant activities. In conclusion B. biternata appeared as an important source for the discovery of new antimicrobial and antioxidant compounds.
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Abdalla AE, Roozen JP (1999) Effect of plant extracts on the oxidative stability of sunflower oil and emulsion. Food Chem 3:323–329
Ali SI (1978) The flora of Pakistan: some general and analytical remarks. Notes R Bot Gard Edinb 36:427–439
Bhat JA, Kumar M, Bussmann RW (2013) Ecological status and traditional knowledge of medicinal plants in Kedarnath Wildlife Sanctuary of Garhwal Himalaya, India. J Ethnobiol Ethnomed 9:1
Bhattacharjee I, Chatterjee SK, Ghosh A, Chandra G (2011) Antibacterial activities of some plant extracts used in Indian traditional folk medicine. Asian Pac J Trop Biomed 1:S165–S169
Boham BA, Kocipai-Abyazam R (1974) Flavonoids and condensed tannins from leaves of Hawaiina vacinium vaticulatum and V. calycinium. Pac Sci 48:458–463
Botzenhardt K, Doring G (1993) Ecology and epidemiology of Pseudomonas aeruginosa. Pseudomonas aeruginosa Oppor Pathog 1–7
Brand-Williams W, Cuvelier ME, Berset C (1995) Use of free radical method to evaluate antioxidant activity. Lebensm Wiss Technol 28:25–30
Fatima N, Zia M, Rehman R, Rizvi ZF, Ahmed S, Mirza B, Chaudhary MF (2009) Biological activities of Rumex dentatus L: evaluation of methanol and hexane extracts. Afr J Biotech 8(24):6945–6951
Ghosh G, Ghosh DC, Melkania U, Majumdar U (2014) Traditional medicinal plants used by the Adi, Idu and Khamba tribes of Dehang-Debang biosphere reserve in Arunachal Pradesh. Int J Agric Environ Biotech 7(1):165–171
Gootz TD (2006) The forgotten gram-negative bacilli: what genetic determinants are telling us about the spread of antibiotic resistance. Biochem Pharmacol 71:1073–1084
Harborne JB (1973) Phytochemical methods. Chapman and Hall, Ltd, London, pp 49–188
Ishikawa NK, Fukushi Y, Yamaji K, Tahara S, Takahashi K (2001) Antimicrobial cuparene-type sesquiterpenes, enokipodins C and D, from a mycelial culture of Flammulina velutipes. J Nat Prod 64:932–934
Jayanthi P, Lalitha P (2011) Reducing power of the solvent extracts of Eichhornia crassipes (mart.) solms. Int J Pharm Sci 3(3):126–128
Kadel C, Jain AK (2006) Folklore claims on snakebite among some tribal communities of central India. Indian J Tradit Know 7(2):296–299
Kala CP (2005) Current status of medicinal plants used by traditional vaidyas in Uttaranchal state of India. Ethnobot Res Appl 3:267–278
Khan AV, Ahmed QU, Mir MR, Shulka I, Khan AA (2012) Antibacterial efficacy of the seed extracts of Melia azedarach against some hospital isolated human pathogenic bacteria strains. Asia Pac J Trop Biomed 1:452–455
Madhumitha G, Saral AM (2011) Preliminary phytochemical analysis, antibacterial, antifungal and anticandidal activities of successive extracts of Crossandra infundibuliformis. Asian Pac J Trop Biomed 4:192–195
Nanda Y, Singson N, Rao AN (2013) Ethnomedicinal plants of Thadou tribe of Manipur (India)—1. Pleione 7(1):138–145
Olajuyigbe O, Afolayan AJ (2012) In vitro pharmacological activity of the crude acetone extract of Erythrina caffra Thunb: antibacterial and antifungal assessment. J Med Plants Res 6(713–1720):26
Panda H (2000) Medicinal plants cultivation and their uses. Asia Pacific Business Press Inc., Delhi, pp 503–505
Panda H (2002) Medicinal plants cultivation and their uses. Asia Pacific Business Press Inc., Delhi, p 505
Pandurangan A, Khosa RL, Hemalatha S (2008) Evaluation of anti-inflammatory and analgesic activity of root extract of Solanum trilobatum Linn. Iran J Pharmaceut Res 7:217–221
Philip DL, Daniel JW, Nancy DH (2009) Antibacterial-resistant Pseudomonas aeruginosa: clinical impact and complex regulation of chromosomally encoded resistance mechanisms. Clin Microbiol Rev 22(4):582–610
Priyadi H, Takao G, Rahmawati I, Supriyanto B, Nursal WI, Rahman I (2010) Five hundred plant species in Gunung Halimun Salak National Park, West Java: a checklist including Sundanese names, distribution and use. CIFOR, Bogor
Ravishankar K, Priya PSV (2012) In vitro antioxidant activity of ethanolic seed extracts of Macrotyloma uniflorum and Cucumis melo for therapeutic potential. Int J Pharm Bio Sci 2(2):442–445
Saad S, Taher M, Susanti D, Qaralleh H, Rahim NABA (2011) Antimicrobial activity of mangrove plant (Lumnitzera littorea). Asian Pac J Trop Med 4:523–525
Sasidharan N, Bhasha SC, Renuka C (2006) Botanical studies in the medicinal plant conservation areas in Kerala: KFRI research report 99. Kerala Forest Research Institute Peechi, Thrissur
Sasidharan S, Prema B, Yoga LL (2011) Antimicrobial drug resistance of Staphylococcus aureus in dairy products. Asian Pac J Trop Biomed 1:130–132
Satish S, Mahesh B (2008) Antimicrobial activity of some important medicinal plants against plant and human pathogens. World J Agric Sci 4:839–843
Shah S, Ram J, Pala AN, Tripathi P, Kumar M (2014) Medicinal plant wealth of oak dominated forests in Nainital catchment area of Uttarakhand. Acad J Med Plants 2(1):006–013
Shahwar D, Ullah S, Raza MA, Sana U, Yasmeen A, Ghafoor S, Ahmad N (2011) Acetylcholine esterase and antioxidant potential of some members of Asteraceae and Euphorbiaceae. J Med Plants Res 5(32):7011–7016
Singleton VL, Rossi JA (1965) Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Eno and Vitic 16:144–158
Sivaraj R, Balakrishnan A, Thenmozhi M, Venkatesh R (2011) Preliminary phytochemical screening of Aegle marmelos, Ruta graveolens, Opuntia dellini, Euphorbia royleana and Euphorbia antiquorum. Int J Pharm Sci Res 2(1):146–150
Sukumaran P, Nair AG, Chinmayee DM, Mini I, Sukumaran ST (2012) Phytochemical investigation of Bidens biternata (Lour.) Merr. and Sheriff.—a nutrient-rich leafy vegetable from Western Ghats of India. Appl Biochem Biotechnol 67:1795–1801
Valgas C, Souza SM, Smânia EFA, Artur SJ (2007) Screening methods to determine antibacterial activity of natural products. Braz J Microbiol 38:369–380
Van Buren JP, Robinson WB (1969) Formation of complexes between protein and tannic acid. J Agric Food Chem 17:772–777
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Zahara, K., Bibi, Y., Qayyum, A. et al. Investigation of Antimicrobial and Antioxidant Properties of Bidens biternata. Iran J Sci Technol Trans Sci 43, 725–734 (2019). https://doi.org/10.1007/s40995-018-0564-2
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DOI: https://doi.org/10.1007/s40995-018-0564-2