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Statistical Optimization of Process Parameters for Bioplastic (PHA) Production by Bacillus subtilis NCDC0671 Using Orange Peel-Based Medium

Abstract

Systematic and sequential optimization strategy based on statistical methodology involving one factor at a time approach was used to enhance PHA production by Bacillus subtilis NCDC0671 using orange peel hydrolysate-based medium in shake flask cultures. Medium components exerting a significant influence in PHA production were screened out using the Plackett–Burman design. Following Box–Behnken method, three significant factors influencing PHA production were identified as beef extract, sodium chloride and incubation period. The optimum concentration and interaction effect of these three components were determined by RSM based on Box–Behnken design. The model developed was validated, and the optimum medium containing 2.23 (g/L) beef extract, 0.72 (g/L) sodium chloride and 48 h of incubation period led to maximum PHA production of 5.09 g/L, which was 2.49-fold higher than the unoptimized medium. The ANOVA results established the significance of the model (p < 0.05). The present investigation innovatively developed a low-cost fermentation medium utilizing orange peels to produce considerable level of PHA.

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Acknowledgements

This research did not receive any specific Grant from funding agencies in the public, commercial, or not-for-profit sectors. We would like to acknowledge the Department of Microbial Biotechnology for providing necessary facilities for carrying out this research work.

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MU, VMM and TB have equally contributed to the planning, execution of work and preparation of manuscript. KP has served as supervisor and guide for the research work and manuscript preparation.

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Correspondence to Mridul Umesh.

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Authors declare no conflict of interest.

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Umesh, M., Mani, V.M., Thazeem, B. et al. Statistical Optimization of Process Parameters for Bioplastic (PHA) Production by Bacillus subtilis NCDC0671 Using Orange Peel-Based Medium. Iran J Sci Technol Trans Sci 42, 1947–1955 (2018). https://doi.org/10.1007/s40995-017-0457-9

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Keywords

  • PHA
  • Plackett–Burman design
  • Response surface methodology
  • Fruit peels
  • Box–Behnken design
  • Bacillus subtilis