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Biodegradation of Selected Polycyclic Aromatic Hydrocarbons by Axenic Bacterial Species Belonging to the Genera Lysinibacillus and Paenibacillus

  • Obinna C. NwinyiEmail author
  • Olukayode O. Amund
Research Paper

Abstract

The quest for competent degraders of recalcitrant polynuclear aromatic hydrocarbons (PAHs) for use in sustainable bioremediation technology has justified the execution of this work. In this study, three bacterial strains (FB-1, FB-2 and FB-3) were isolated from a former industrial site in Bloomington, Indiana. The catabolic versatility of these obtained strains was evaluated on some selected PAH-naphthalene, anthracene, fluoranthene and pyrene. Using the 16S rRNA sequencing analyses, our strains belonged to the family Firmicutes whereby strain FB-1 was identified as Lysinibacillus sp. FB-1, strain FB-2 as Bacterium FB-2 and strain FB-3 as Lysinibacillus fusiformis FB-3. The biodegradation of the selected PAHs was determined using gas chromatography, and the calculated percentage utilization of the selected PAHs varied between 97 and 4%. We further determined the mean biodegradation rates for fluoranthene when supplemented with molasses. The mean biodegradation rates were between (mg L−1) 0.214 ± 0.006 and 0.318 ± 0.002, while MS-fluoranthene only ranged from (mg L−1) 0.210 ± 0.056 to 0.437 ± 0.176. However, with ANOVA at 5% (P < 0.05) there seemed to be no significant difference in the mean biodegradation rates between both media. These findings may have practical and ecological prospects in designing and improving bioremediation of polycyclic aromatic hydrocarbon contaminated sites.

Keywords

Competent degraders Bioremediation Molasses PAHs 16S rRNA 

Notes

Acknowledgements

The authors would like to acknowledge the financial support of Institute of International Education, administrators of the Fulbright Scholarship which supported O. Nwinyi and the School of Public and Environmental Affairs Indiana University, Bloomington IN, USA, for additional support. The authors appreciate the inputs of Associate Professor Flynn W. Picardal and An Thuy of the School of Public and Environmental Affairs Indiana University, Bloomington IN, USA.

Compliance with ethical standards

Conflict of interest

Authors have declared that no competing interests exist.

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Copyright information

© Shiraz University 2017

Authors and Affiliations

  1. 1.Department of Biological Sciences, College of Science and TechnologyCovenant UniversityOtaNigeria
  2. 2.Department of MicrobiologyUniversity of LagosLagosNigeria
  3. 3.Department of Biotechnology and Food TechnologyUniversity of JohannesburgDoornfonteinSouth Africa

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