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Analysis of Antimicrobial Activities of Different Parts of Symplocos racemosa: an Endangered Medicinal Plant of Eastern Ghats of India

Abstract

Antimicrobial activity of ethanol, methanol, and chloroform extracts from bark, leaves, and roots of Symplocos racemosa, an Indian medicinal plant, was examined against eight species of pathogenic and non-pathogenic microorganisms: Escherichia coli, Bacillus subtilis, Klebsiella pneumonia, Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa, Aspergillus niger, and Candian albican using disc diffusion method. All the extracts from different parts of the plant exhibit antimicrobial activity against most tested microorganisms. The maximum level of antibacterial activity was observed in bark extract against S. aureus (32.95 ± 0.88 mm) at the concentration of 20 mg of methanol extract and the minimum activity was observed against P. aeruginosa (1.83 ± 0.13 mm) at the concentration of 5 mg of chloroform extract. The methanol extract of root of S. racemosa showed highest antibacterial activity against S. aureus (30.75 ± 0.83 mm) at the concentration of 20 mg of extract, but less activity was observed against P. aeruginosa (2.05 ± 0.13 mm) at the concentration of 5 mg of extract. Methanol leaf extract of S. racemosa showed more activity against S. aureus (30.75 ± 0.83 mm) followed by E. faecalis (19.00 ± 0.68 mm) at the concentration of 20 mg and less activity was observed against P. aeruginosa, B. subtilis, and A. niger. Hence, the isolation of antimicrobial compounds from root, bark, and leaf of S. racemosa with possible mechanism would be a better option for the synthesis of new antimicrobial drugs to treat infectious diseases caused by microorganisms.

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References

  • Behera SK, Misra MK (2005) Indigenous phytotherapy for genito-urinary diseases used by the Kirk- Kandha tribe of Orissa India. J Ethnopharmacol 102:319–325

    Article  Google Scholar 

  • Bourke CA, Carrigan MJ, Dixon RJ (1990) Upper motor neuron effects in sheep of some betacarotine alkaloids identified in Zygophyllaceous plants. Aust Vet J 67:248–251

    Article  Google Scholar 

  • Bylka W, Szaufer-Hajdrych M, Matalawskan I et al (2003) Antimicrobial activity of isocytisoside and extracts of Aquilegia vulgaris L. Appl Microbiol 39:3–97

    Google Scholar 

  • Chopra RN, Chopra IC, Handa KL et al (1958) Indigenous Drugs of India, U.N.Dhur & Sons Pvt. Ltd., Calcutta, 1958; pp 413, 606, 608, 610, 686

  • Dayal KS (1933) Vadic Mukhzanul Mufredaat, IInd Kutub Khana Anjuman, Taraqqi Urdu Bazar, Delhi. 222

  • Devmurari V (2002) Phytochemical screening and evaluation of antioxidant activity of Symplocos racemosa Roxb. J Adv Sci Res 1:28–34

    Google Scholar 

  • Dymock W, Warden CJH, Hooper D (1891) Pharmacographia indica. vol 11. Thacker Spink & Co., Calcutta, pp 373–375

  • Evans WC (2003) Trease and Evans Pharmacognosy, 14th edn. WB Saunders Company Ltd., London. UK, p 480

  • Farooq S (2005) 555 Medicinal plants, field and laboratory manual, vol 1. International book distributors, Dehradun, pp 250–251

    Google Scholar 

  • Govindarajan R, Vijayakumar M, Singh M et al (2006) Antiulcer and antimicrobial activity of Anogeissus latifolia. J Ethnopharmacol 61(106):57

    Article  Google Scholar 

  • Gupta N, Haque A, Lattif A (2004) Epidemiology and molecular typing of Candida isolates from burn patients. Mycopathologia 158(4):397–405

    Article  Google Scholar 

  • Haleem A (1984) Mufridat-e-Azizi. 4th edn. Sahet-e-Mandir Press Ltd., Lucknow. p 12

  • Hasan M (1984) Tauzehul Advia. Gulzar Mohammadi Press, Meerut, p 163

    Google Scholar 

  • Jayaraman SK, Manoharan MS, Illanchezian S (2008) Antibacterial, antifungal and tumor cellsuppression potential of Morinda citrifolia fruit extracts. Int J Integr Biol 3:44–49

    Google Scholar 

  • Karaman I, Sahin F, Gulluce M, Ogutcu H, Sengul M, Adiguzel A (2003) Antimicrobial activity of aqueous and methanol extracts of Juniperus oxycedrus L. J Ethnopharmacol 2837:1–5

    Google Scholar 

  • Khan MA, Muheet-e-Azam., Matba Nizami (1982) Kanpur. vol.4, Part 2, pp. 21–22

  • Khory RN, Katrak NN (1985) Materia Medica of India and their therapeutics. Neeraj Publishing House, Delhi, pp 432–433

    Google Scholar 

  • Kostova I, Dinchev D (2005) Saponins in Tribulus ter restris—chemistry and bioactivity. Phytochem Rev 4(2–3):111–137

    Article  Google Scholar 

  • Kumaraswamy Y, Cox PJ, Jaspars M et al (2002) Screening seeds of scottish plants for antibacterial activity. J Ethnopharmacol 83:73–77

    Article  Google Scholar 

  • Mothana RA, Lindequist U (2005) Antimicrobial activity of some medicinal plants of the island Soqotra. J Ethnopharmacol 96(1–2):177–181

    Article  Google Scholar 

  • Mshvildadze V, Favel A, Delmas F (2000) Antifungal and antiprotozoal activities of saponins from Hedera colchica. Pharmazie 55(4):325–326

    Google Scholar 

  • Nadkarni KM (2000) Indian Materia Medica. vol 1 Popular Prakashan, Bombay. p 282

  • Palombo EA, Semple SJ (2001) Antibacterial activity of traditional medicinal plants. J Ethnopharmacol 77:151–157

    Article  Google Scholar 

  • Rastogi RP, Mehrotra BN (2002) Glossary of Indian medicinal plants. National Institute of Science Communication, New Delhi. pp 20–25

  • Saleh NA, Ahmed AA, Abdalla MF (1982) Flavonoid glycosides of Tribulus pentandrus and T. terrestris. Phytochemistry 21(8):1995–2000

    Article  Google Scholar 

  • Samy RP, Ignacimuthu S (2000) Antibacterial activity of some folklore medicinal plants used by tribals in Western Ghats in India. J Ethnopharmacol 69:63–71

    Article  Google Scholar 

  • Stepanovic S, Antic N, Dakic I et al (2003) In vitro antimicrobial activity of propilis and antimicrobial drugs. Microbiol Res 158:353–357

    Article  Google Scholar 

  • Tona L, Kambu K, Ngimbi N et al (1988) Antiamoebic and phytochemical screening of some Congolese medicinal plants. J Ethnopharmacol 61:57–65

    Article  Google Scholar 

  • Wei LS, Musa N, Sengm CT, Wee W, Mohd Shazili NA (2008) Antimicrobial properties of tropical plants against 12 pathogenic bacteria isolated from aquatic organisms. Afr J Biotechnol 7:2275–2278

    Google Scholar 

  • Williamson EM (2002) Major Herbs of Ayurveda. Churchill-Livingstone, China. p 312

  • Xu YX, Chen HS, Liang HQ (2000) Three new saponins from Tribulus terrestris. Planta Med 66(6):545–550

    Article  Google Scholar 

  • Zhang JD, Xu Z, Cao YB et al (2006) Antifungal activities and action mechanisms of compounds from Tribulus terrestris L. J Ethnopharmacol 103(1):76–84

    Article  Google Scholar 

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Acknowledgments

The authors are grateful to Prof (Dr). Sudam Chandra Si, Dean and Prof (Dr). Manoj Ranjan Nayak, President, Centre of Biotechnology, Siksha O Anusandhan University, for providing all facilities. Financial assistance from DBT, New Delhi is acknowledged. The authors are also grateful to Dean and President, Orissa University of Agriculture and Technology for providing financial support and encouraging throughout. The authors are thankful to Dr P C Panda, RPRC for identification of the sample.

Funding was provided by Department of Biotechnology, Ministry of Science and Technology.

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Correspondence to Manoj Kumar Panda or Pratap K. Pattnaik.

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Kar, D., kuanar, A., Panda, M.K. et al. Analysis of Antimicrobial Activities of Different Parts of Symplocos racemosa: an Endangered Medicinal Plant of Eastern Ghats of India. Iran J Sci Technol Trans Sci 42, 1077–1085 (2018). https://doi.org/10.1007/s40995-016-0118-4

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  • DOI: https://doi.org/10.1007/s40995-016-0118-4

Keywords

  • Symplocos racemosa
  • Antimicrobial
  • Antifungal
  • E. coli