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Energy, Ecology and Environment

, Volume 3, Issue 2, pp 126–135 | Cite as

Combinatorial application of ammonium carbonate and sulphuric acid pretreatment to achieve enhanced sugar yield from pine needle biomass for potential biofuel–ethanol production

  • Surbhi Vaid
  • Neha Bhat
  • Parushi Nargotra
  • Bijender Kumar Bajaj
Original Article

Abstract

Lignocellulosic biomass (LB) despite its huge potential as a renewable bioenergy resource faces bottlenecks due to its recalcitrance and lack of appropriate pretreatment approaches. The current study evaluates the combinatorial application of alkali and acid pretreatment of pine needle biomass (PNB), for achieving high sugar release upon enzymatic saccharification. Pine needle accumulation poses a big threat to the forest soil fertility and overall ecosystem and environment. However, pine needle waste can be valorized after appropriate pretreatment and enzymatic saccharification for production of renewable energy, i.e. biofuel–ethanol. In combinatorial pretreatment strategy, first PNB was subjected to ammonium carbonate pretreatment, and parameters like ammonium carbonate concentration, incubation time and pretreatment temperature were optimized using design of experiment (DoE) approach. The relative influence of parameters on efficacy of pretreatment was established individually and in interactive terms. Based on DoE, sugar yield of 7.56 mg/g of PNB was obtained. Furthermore, DoE-based pretreated PNB was subjected to sulphuric acid pretreatment, followed by enzymatic saccharification. The sugar released during various steps was pooled (8.19 g/100 g), concentrated and subjected to ethanol fermentation with dual yeast cultures using Saccharomyces cerevisiae and Pichia stipitis. An ethanol yield of 8.8%, v/v (6.94% w/v), was obtained. This represents the process efficiency of 19.34% for bioethanol production from PNB.

Keywords

Pine needle biomass Ammonium carbonate–sulphuric acid pretreatment Enzymatic saccharification Fermentation Bioethanol 

Notes

Acknowledgements

Dr. Bijender Kumar (Bajaj) gratefully acknowledges the Institute of Advanced Study, Durham University, UK, for providing COFUND International Senior Research Fellowship for ‘Research Stay’ at Department of Biosciences, Durham University, Durham, UK; Department of Science and Technology (Govt. of India) is acknowledged for financial support (Research Project Ref. SR/SO/BB-66/2007), and Commonwealth Scholarship Commission, UK, for providing Commonwealth Fellowship (INCF-2013-45) for ‘Research Stay’ at Institute of Biological, Environmental and Rural Sciences (IBERS), Aberystwyth University, Aberystwyth, UK. Authors thank the Director, School of Biotechnology, University of Jammu, Jammu, for necessary laboratory facilities.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. Alvira P, Tomas-Pejo E, Ballesteros M, Negro MJ (2010) Pretreatment technologies for an efficient bioethanol production process based on enzymatic hydrolysis: a review. Bioresour Technol 101:4851–4861CrossRefGoogle Scholar
  2. Choi WI, Park JY, Lee JP, Oh YK, Park YC, Kim JS, Park JM, Kim CH, Lee JS (2013) Optimization of NaOH-catalyzed steam pretreatment of empty fruit bunch. Biotechnol Biofuel 6:170CrossRefGoogle Scholar
  3. de Souza ROMA, Mirandaa LSM, Luque R (2014) Bio(chemo)technological strategies for biomass conversion into bioethanol and key carboxylic acids. Green Chem 16:2386CrossRefGoogle Scholar
  4. Gao W, Tabil LG, Dumonceaux T, Ríos SE, Zhao R (2017) Optimization of biological pretreatment to enhance the quality of wheat straw pellets. Biomass Bioenergy 97:77–89CrossRefGoogle Scholar
  5. Ghosh MK, Ghosh UK (2011) Utilization of pine needles as bed material in solid state fermentation for production of lactic acid by lactobacillus strains. BioResources 6:1556–1575Google Scholar
  6. Guo X, Cavka A, Jonsson LJ, Hong F (2013) Comparison of methods for detoxification of spruce hydrolysate for bacterial cellulose production. Microb Cell Fact 12:1CrossRefGoogle Scholar
  7. Gupta P, Samant K, Sahu A (2012) Isolation of cellulose-degrading bacteria and determination of their cellulolytic potential. Int J Microbiol.  https://doi.org/10.1155/2012/578925 Google Scholar
  8. He YC, Liu F, Gong L, Lu T, Ding Y, Zhang Dan-Ping, Qing Q, Zhang Y (2015) Improving enzymatic hydrolysis of corn stover pretreated by ethylene glycol-perchloric acid-water mixture. Appl Biochem Biotechnol 175:1306–1317CrossRefGoogle Scholar
  9. Hou Q, Ju M, Li W, Liu L, Chen Y, Yang Q (2017) Pretreatment of lignocellulosic biomass with ionic liquids and ionic liquid-based solvent systems. Molecules 22:490CrossRefGoogle Scholar
  10. Jin S, Zhang G, Zhang P, Fan S, Li F (2015) High-pressure homogenization pretreatment of four different lignocellulosic biomass for enhancing enzymatic digestibility. Bioresour Technol 181:270–274CrossRefGoogle Scholar
  11. Jonsson LJ, Martin C (2016) Pretreatment of lignocellulose: formation of inhibitory by-products and strategies for minimizing their effects. Bioresour Technol 199:103–112CrossRefGoogle Scholar
  12. Jonsson LJ, Alriksson B, Nilvebrant NO (2013) Bioconversion of lignocellulose: inhibitors and detoxification. Biotechnol Biofuel 6:1.  https://doi.org/10.1186/1754-6834-6-16 CrossRefGoogle Scholar
  13. Kang Q, Appels L, Tan T, Dewil R (2014) Bioethanol from lignocellulosic biomass: current findings determine research priorities. Sci World J.  https://doi.org/10.1155/2014/298153 Google Scholar
  14. Karcher MA, Iqbal Y, Lewandowski T (2015) Comparing the performance of Miscanthus giganteus and wheat straw biomass in sulfuric acid based pretreatment. Bioresour Technol 180:360–364CrossRefGoogle Scholar
  15. Kim I, Lee B, Song D, Han JI (2014) Effects of ammonium carbonate pretreatment on the enzymatic digestibility and structural features of rice straw. Bioresour Technol 166:353–357CrossRefGoogle Scholar
  16. Kim JS, Lee YY, Kim TH (2016) A review on alkaline pretreatment technology for bioconversion of lignocellulosic biomass. Bioresour Technol 199:42–48CrossRefGoogle Scholar
  17. Kumar R, Tabatabaei M, Karimi K, Sárvári Horváth I (2016) Recent updates on lignocellulosic biomass derived ethanol—a review. Biofuel Res J 9:347–356CrossRefGoogle Scholar
  18. Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426–428CrossRefGoogle Scholar
  19. Mohan M, Banerjee T, Goud VV (2015) Hydrolysis of bamboo biomass by subcritical water treatment. Bioresour Technol 191:244–252CrossRefGoogle Scholar
  20. Nanda S, Dalai AK, Kozinski JA (2014) Butanol and ethanol production from lignocellulosic feedstock: biomass pretreatment and bioconversion. Energy Sci Eng 2:138–148CrossRefGoogle Scholar
  21. Nargotra P, Vaid S, Bajaj BK (2016) Cellulase production from Bacillus subtilis SV1 and its application potential for saccharification of ionic liquid pretreated pine needle biomass under one pot consolidated bioprocess. Fermentation 2:19CrossRefGoogle Scholar
  22. Novy V, Longus K, Nidetzky B (2015) From wheat straw to bioethanol: integrative analysis of a separate hydrolysis and co-fermentation process with implemented enzyme production. Biotechnol Biofuel 8:46CrossRefGoogle Scholar
  23. Oladi S, Aita GM (2017) Optimization of liquid ammonia pretreatment variables for maximum enzymatic hydrolysis yield of energy cane bagasse. Ind Crops Product 103:122–132CrossRefGoogle Scholar
  24. Pandey AK, Negi S (2015) Impact of surfactant assisted acid and alkali pretreatment on lignocellulosic structure of pine foliage and optimization of its saccharification parameters using response surface methodology. Bioresour Technol 192:115–125CrossRefGoogle Scholar
  25. Phitsuwan P, Permsriburasuk C, Waeonkul R, Pason P, Tachaapaikoon C, Ratankhanokchai K (2016) Evaluation of fuel ethanol production from aqueous ammonia-treated rice straw via simultaneous saccharification and fermentation. Biomass Bioenergy 150:150–157CrossRefGoogle Scholar
  26. Rabemanolontsoa H, Saka S (2016) Various pretreatments of lignocellulosics. Bioresour Technol 199:83–91CrossRefGoogle Scholar
  27. Sharma M, Bajaj BK (2017) Optimization of bioprocess variables for production of a thermostable and wide range pH stable carboxymethyl cellulase from Bacillus subtilis MS 54 under solid state fermentation. Environ Prog Sustain Energy.  https://doi.org/10.1002/ep.12557 Google Scholar
  28. Sindhu R, Binod P, Mathew AK, Abraham A, Gnansounou E, Ummalyma SB, Thomas L, Pandey A (2017) Development of a novel ultrasound-assisted alkali pretreatment strategy for the production of bioethanol and xylanases from chili post harvest residue. Bioresour Technol.  https://doi.org/10.1016/j.biortech.2017.03.001 Google Scholar
  29. Singh S, Anu, Vaid S, Singh P, Bajaj BK (2016) Physicochemical pretreatment of pine needle biomass by design of experiments approach for efficient enzymatic saccharification. J Mater Environ Sci 7:2034–2041Google Scholar
  30. Teramura H, Sasaki K, Oshima T, Matsuda F, Okamoto M, Shirai T, Kawaguchi H, Ogino C, Hirano K, Sazuka T, Kitano H (2016) Organosolv pretreatment of sorghum bagasse using a low concentration of hydrophobic solvents such as 1-butanol or 1-pentanol. Biotechnol Biofuel 9:27CrossRefGoogle Scholar
  31. Tian D, Chandra RP, Lee JS, Lu C, Saddler JN (2017) A comparison of various lignin-extraction methods to enhance the accessibility and ease of enzymatic hydrolysis of the cellulosic component of steam-pretreated poplar. Biotechnol Biofuel 10:157CrossRefGoogle Scholar
  32. Timung R, Mohan M, Chilukoti B, Sasmal S, Banerjee T, Goud VV (2015) Optimization of dilute acid and hot water pretreatment of different lignocellulosic biomass: a comparative study. Biomass Bioenerg 81:9–18CrossRefGoogle Scholar
  33. Vaid S, Bajaj BK (2017) Production of ionic liquid tolerant cellulase from Bacillus subtilis G2 using agroindustrial residues with application potential for saccharification of biomass under one pot consolidated bioprocess. Waste Biomass Valor 8:949–964CrossRefGoogle Scholar
  34. Vaid S, Nargotra P, Bajaj BK (2017) Consolidated bioprocessing for biofuel-ethanol production from pine needle biomass. Environ Prog Sustain Energy.  https://doi.org/10.1002/ep.12691 Google Scholar
  35. Vats S, Maurya DP, Jain A, Mall V, Negi S (2013) Mathematical model-based optimization of physico-enzymatic hydrolysis of Pinus roxburghii needles for the production of reducing sugars. Indian J Exp Biol 51:944–953Google Scholar
  36. Vogel KP, Dien BS, Jung HG, Casler MD, Masterson SD, Mitchell RB (2011) Quantifying actual and theoretical ethanol yields for switchgrass strains using NIRS analyses. Bioenergy Res 4:96–110CrossRefGoogle Scholar
  37. Wi SG, Cho EJ, Lee DS, Lee SJ, Lee YJ, Bae HJ (2015) Lignocellulose conversion for biofuel: a new pretreatment greatly improves downstream biocatalytic hydrolysis of various lignocellulosic materials. Biotechnol Biofuel 8:228CrossRefGoogle Scholar
  38. Yadav SK, Naseeruddin S, Prashanthi SG, Sateesh L, Rao VL (2012) Bioethanol fermentation of concentrated rice straw hydrolysate using co-culture of Saccharomyces cerevisiae and Pichia stipitis. Bioresour Technol 102:6473–6478CrossRefGoogle Scholar

Copyright information

© Joint Center on Global Change and Earth System Science of the University of Maryland and Beijing Normal University and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Surbhi Vaid
    • 1
  • Neha Bhat
    • 1
  • Parushi Nargotra
    • 1
  • Bijender Kumar Bajaj
    • 1
    • 2
  1. 1.School of BiotechnologyUniversity of JammuJammuIndia
  2. 2.Department of BiosciencesDurham UniversityDurhamUK

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