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Optimization of environmental conditions for the growth and bioaccumulation of As(III) and As(V) ions by growing Corynebacterium glutamicum MTCC 2745: inhibition kinetic study

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Abstract

The significant factors affecting simultaneously the growth and bioaccumulation of arsenic [As(III) and As(V)] ions by living cells of bacteria, Corynebacterium glutamicum MTCC 2745, were explored in growth media under various experimental environmental conditions such as pH, inoculum size, contact time, temperature, concentrations of peptone and arsenic [either As(III) or As(V)] ions. Three kinetic models namely competitive, noncompetitive and uncompetitive models were verified with the aim of deciding the toxic effects of arsenic on specific growth rate of C. glutamicum MTCC 2745. As(III) was observed to be more toxic than As(V). Competitive inhibition was found in either As(III) or As(V) systems which was suggested by almost same maximum specific growth rate (µ m) value and an increase in the saturation constant (K′S,app) value that were evaluated from Lineweaver–Burk plots. A simultaneous reduction in the inhibition constant recommended that inhibitory effect increases with the increase in arsenic concentration.

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Abbreviations

C 0 :

Initial metal concentration (mg/L)

C f :

Final metal concentration (mg/L)

I :

Concentrations of the inhibitory component (mg/L)

K I :

Inhibition constant (mg/L)

K S :

Half velocity constant or saturation constant or Monod constant (g/L)

K′S,app :

Apparent saturation constant (mg/L)

M :

Concentrations of microorganism (g/L)

S :

Concentration of substrate (g/L)

S 0 :

Initial peptone concentration (g/L)

t :

Incubation time (h)

T :

Absolute temperature (°C)

X :

Concentrations of dried cell mass (g/L)

X m :

Maximum biomass concentration (g/L)

X 1 :

Initial concentration of peptone (g/L)

X 2 :

Initial concentration of arsenic [either As(III) or As(V)] ions (mg/L)

µ :

Specific growth rate of the microorganisms (h−1)

µ m :

Maximum specific growth rate of the microorganisms (h−1) when S ≫ K S

µ m,app :

Apparent maximum specific growth rate of the microorganisms (h−1)

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Acknowledgements

Our thanks to Indian Institute of Technology, Roorkee, for providing necessary facilities and to Ministry of Human Resource Development, Government of India, for financial support. The thoughtful comments by Dr. Jim LaMoreaux, the Editor in Chief and one anonymous reviewer are highly appreciated.

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  1. Department of Chemical Engineering, Indian Institute of Technology, Roorkee, 247667, India

    M. S. Podder & C. B. Majumder

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Podder, M.S., Majumder, C.B. Optimization of environmental conditions for the growth and bioaccumulation of As(III) and As(V) ions by growing Corynebacterium glutamicum MTCC 2745: inhibition kinetic study. Sustain. Water Resour. Manag. 4, 23–44 (2018). https://doi.org/10.1007/s40899-017-0114-7

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